【摘要】：膜片鉗技術(shù)現(xiàn)在已經(jīng)成為電生理領(lǐng)域的主要技術(shù)手段，與分子生物學(xué)、細(xì)胞生物學(xué)、分子遺傳學(xué)、生理學(xué)、病理學(xué)和藥理學(xué)等學(xué)科都有著密切聯(lián)系。國際上領(lǐng)先的膜片鉗系統(tǒng)正在向高通量、工業(yè)化、全自動(dòng)化的方向發(fā)展。目前世界上首個(gè)擁有48通道的高通量全自動(dòng)膜片鉗系統(tǒng)是由丹麥的Sophion公司推出的QPatch HT系統(tǒng)。它每天可記錄250~1200個(gè)數(shù)據(jù)物。目前我們使用的是PC2C型膜片鉗放大器，與之配套的有UDA-1型/UDA-2型數(shù)據(jù)采集系統(tǒng)，和膜片鉗系統(tǒng)控制軟件PClamp4.0。我的主要工作是在膜片鉗系統(tǒng)控制軟件PClamp4.0的基礎(chǔ)上設(shè)計(jì)并調(diào)試應(yīng)用程序使之與新研發(fā)的UDA-2型數(shù)據(jù)采集系統(tǒng)匹配。本文主要論述了UDA-2數(shù)據(jù)采集系統(tǒng)的特點(diǎn)和優(yōu)勢，以及針對(duì)UDA-2數(shù)據(jù)采集系統(tǒng)設(shè)計(jì)的模塊，和詳細(xì)的實(shí)現(xiàn)方法。 本文首先以整體的膜片鉗系統(tǒng)結(jié)構(gòu)入手，，介紹膜片鉗系統(tǒng)的軟件、硬件組成部分。以應(yīng)用程序內(nèi)核框架為主，分別介紹內(nèi)核框架中刺激波形產(chǎn)生模塊，刺激波形發(fā)送數(shù)據(jù)采集模塊、數(shù)據(jù)處理模塊、端口旋鈕監(jiān)控模塊等七個(gè)模塊的功能，以及各個(gè)模塊之間的相互關(guān)系。針對(duì)UDA-2數(shù)據(jù)采集系統(tǒng)，詳細(xì)說明數(shù)據(jù)系統(tǒng)的啟動(dòng)、刺激波形產(chǎn)生模塊、刺激波形發(fā)送數(shù)據(jù)采集模塊以及端口監(jiān)控模塊的功能具體實(shí)現(xiàn)過程。 升級(jí)的應(yīng)用程序在調(diào)試過程中出現(xiàn)幾個(gè)問題：兼容UDA-1型和UDA-2型這兩種數(shù)據(jù)采集系統(tǒng)的方法、顯示的數(shù)據(jù)波形出現(xiàn)毛刺以及調(diào)試中出現(xiàn)編譯錯(cuò)誤。本文中對(duì)錯(cuò)誤原因進(jìn)行分析，給出相應(yīng)的解決方案。最后對(duì)全文內(nèi)容進(jìn)行總結(jié)，并對(duì)未來進(jìn)行展望。
[Abstract]:Patch clamp technology has become the main technology in the field of electrophysiology, and has a close relationship with molecular biology, cell biology, molecular genetics, physiology, pathology and pharmacology. The world's leading patch-clamp systems are developing towards high throughput, industrialization, and full automation. The world's first 48-channel high-throughput automatic patch clamp system is the QPatch HT system introduced by Denmark's Sophion Company. It can record between 250 and 1200 data a day. At present, we use the PC2C patch-clamp amplifier, with the UDA-1 / UDA-2 data acquisition system, and the patch clamp system control software PClamp4.0. My main work is to design and debug the application program based on the patch-clamp system control software PClamp4.0 to match the newly developed UDA-2 data acquisition system. This paper mainly discusses the characteristics and advantages of UDA-2 data acquisition system, as well as the design module of UDA-2 data acquisition system and the detailed implementation method. In this paper, the whole structure of patch clamp system is introduced, and the software and hardware of patch clamp system are introduced. Based on the kernel framework of application program, the functions of seven modules, such as stimulation waveform generation module, stimulating waveform sending data acquisition module, data processing module, port knob monitoring module and so on, are introduced respectively. And the relationship between each module. For the UDA-2 data acquisition system, the function realization process of the data system starting, stimulating waveform generating module, stimulating waveform sending data acquisition module and port monitoring module is described in detail. There are several problems in the debugging process of the upgraded application: the methods of compatible UDA-1 and UDA-2 data acquisition system, the burr of the displayed data waveform and the compilation errors in debugging. In this paper, the causes of errors are analyzed, and the corresponding solutions are given. Finally, the full text of the content of the summary, and prospects for the future.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R318.6;TP274.2

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本文編號(hào)：2152089