本文選題：生物相容性材料 + 骨再生��； 參考：《中國組織工程研究》2017年14期

【摘要】：背景:前期實(shí)驗(yàn)驗(yàn)證了富血小板纖維蛋白具有成骨的效果,并且選定了人與兔改良型富血小板纖維蛋白膜結(jié)構(gòu)最相似時(shí)的轉(zhuǎn)速和離心時(shí)間。目的:觀察改良型富血小板纖維蛋白在誘導(dǎo)成骨中的組織學(xué)變化。方法:隨機(jī)將30只日本大耳兔分為2組各15只,在兔顱頂骨制造圓形全層骨缺損來構(gòu)建動(dòng)物模型,改良型富血小板纖維蛋白組填入改良型富血小板纖維蛋白膜,空白組不填任何材料,于建模后當(dāng)天、2周、4周、8周、12周處死動(dòng)物,大體觀察造模區(qū)成骨情況,蘇木精-伊紅染色、masson三色染色、免疫組織化學(xué)觀察造模區(qū)組織學(xué)變化。結(jié)果與結(jié)論:(1)空白組骨缺損在實(shí)驗(yàn)結(jié)束時(shí)不能自行愈合;(2)建模后當(dāng)天、4周、8周、12周2組標(biāo)本的大體觀察、組織學(xué)檢查,均顯示改良型富血小板纖維蛋白組的成骨速度、量及成熟度,皆明顯優(yōu)于空白組,差異具有統(tǒng)計(jì)學(xué)意義;(3)結(jié)果說明,制備的骨缺損實(shí)驗(yàn)期內(nèi)不能自行愈合,符合臨界性骨缺損的標(biāo)準(zhǔn);改良型富血小板纖維蛋白能促進(jìn)新骨形成,隨著時(shí)間的延長,成骨數(shù)量更多,質(zhì)量更佳;改良型富血小板纖維蛋白促使破骨細(xì)胞與成骨細(xì)胞協(xié)同作用,促進(jìn)新骨生成。
[Abstract]:Background: previous experiments have proved that platelet-rich fibrin has the effect of osteogenesis and selected the speed and centrifugation time when the structure of the modified platelet-rich fibrin membrane is the most similar between human and rabbit. Objective: to observe the histological changes of modified platelet-rich fibrin in osteogenesis. Methods: thirty Japanese large ear rabbits were randomly divided into two groups, 15 rabbits in each group. The animal model was established by making a round full-thickness bone defect in the parietal bone of the rabbit. The modified platelet-rich fibrin group was filled with the modified platelet-rich fibrin membrane. In the blank group, the animals were killed at 2 weeks, 4 weeks, 8 weeks and 12 weeks after modeling. The osteogenesis of the model area was observed, the hematoxylin-eosin staining was stained with three-color staining, and the histological changes of the model-making area were observed by immunohistochemistry Results and conclusion: (1) the bone defect in the blank group could not heal by itself at the end of the experiment. (2) the gross observation and histological examination of the two groups showed the rate of osteogenesis in the modified platelet-rich fibrin group. The quantity and maturity were significantly better than the blank group, the difference was statistically significant. (3) the results showed that the prepared bone defect could not heal itself during the experimental period, which met the criteria of critical bone defect. Modified platelet-rich fibrin can promote the formation of new bone, and with the extension of time, the quantity and quality of osteogenesis are more and better, and the modified platelet-rich fibrin can promote osteoclast and osteoblast synergism and promote the formation of new bone.
【作者單位】： 西南醫(yī)科大學(xué)口腔醫(yī)學(xué)院;解放軍成都軍區(qū)機(jī)關(guān)醫(yī)院·成都軍區(qū)口腔種植與修復(fù)臨床醫(yī)學(xué)專科中心;資陽市雁江區(qū)人民醫(yī)院口腔科;
【基金】：全軍“十二五”科研面上課題(CWS11J024)~~
【分類號】：R318.08;R68

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