本文選題：聚四氟乙烯 + 人工血管��； 參考：《中國(guó)組織工程研究》2016年47期

【摘要】：背景:聚四氟乙烯人工血管是臨床常用的醫(yī)用材料,但其具體的基礎(chǔ)研究?jī)?nèi)容尚未完善。目的:觀察聚四氟乙烯人工血管對(duì)血小板聚集的作用及細(xì)胞相容性。方法:(1)對(duì)血小板聚集的作用:檢測(cè)聚四氟乙烯人工血管的凝血時(shí)間、復(fù)鈣時(shí)間。將生理鹽水、蒸餾水及聚四氟乙烯人工血管分別加入兔紅細(xì)胞懸液中,計(jì)算溶血率;(2)細(xì)胞生物相容性:將SD大鼠原代心肌細(xì)胞分別接種于普通玻璃爬片與聚四氟乙烯人工血管片上,3 d后,檢測(cè)細(xì)胞乳酸脫氫酶釋放;5 d后,檢測(cè)細(xì)胞增殖。結(jié)果與結(jié)論:(1)聚四氟乙烯人工血管對(duì)兔血的動(dòng)態(tài)凝血時(shí)間、復(fù)鈣時(shí)間沒有影響;聚四氟乙烯人工血管的溶血率為2.3%;(2)與接種于普通玻璃爬片上的心肌細(xì)胞相比,接種于聚四氟乙烯人工血管片上的心肌細(xì)胞增殖及乳酸脫氫酶釋放無明顯變化;(3)結(jié)果表明,聚四氟乙烯人工血管血小板聚集無影響,具有良好的血液相容性與細(xì)胞相容性。
[Abstract]:Background: polytetrafluoroethylene (PTFE) prosthesis is a commonly used medical material, but its basic research content is not perfect. Objective: to observe the effect of polytetrafluoroethylene (PTFE) on platelet aggregation and its cytocompatibility. Methods the effect of 1) on platelet aggregation: the coagulation time and calcium recovery time of polytetrafluoroethylene (PTFE) artificial blood vessel were measured. Normal saline, distilled water and polytetrafluoroethylene (PTFE) artificial blood vessels were added to rabbit erythrocyte suspension respectively. Cell biocompatibility: SD rat primary cardiomyocytes were inoculated on glass creeping tablets and polytetrafluoroethylene (PTFE) artificial vascular tablets for 3 days, then the lactate dehydrogenase (LDH) was released for 5 days and cell proliferation was detected. The results showed that PTFE prosthesis had no effect on the dynamic coagulation time and calcium recovery time of rabbit blood, and the hemolysis rate of PTFE artificial blood vessel was 2. 3%) compared with that of cardiomyocytes inoculated on ordinary glass creeping tablets. The results showed that the platelet aggregation of PTFE artificial blood vessel had no effect on the proliferation of cardiomyocytes and the release of lactate dehydrogenase (LDH). The results showed that PTFE artificial blood vessel had good blood compatibility and cytocompatibility.
【作者單位】： 鄭州大學(xué)第一附屬醫(yī)院心血管內(nèi)科;
【基金】：河南省教育廳項(xiàng)目(142300410356)~~
【分類號(hào)】：R318.1

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