本文選題：肽核酸芯片 + 光導原位合成��； 參考：《中南大學》2012年碩士論文

【摘要】：肽核酸作為DNA的類似物,在與相應的DNA或RNA序列雜交反應時,表現(xiàn)出更高的特異性和更好的穩(wěn)定性。因此由肽核酸單體合成的肽核酸芯片作為一種新型的基因芯片,近年來在各種基因研究中得到了廣泛的應用。在肽核酸芯片的幾種合成方法中,光導原位合成技術以其合成速度快、芯片密度高、成本低等優(yōu)點表現(xiàn)出全新的競爭力。而微流體反應器作為光導原位合成的場所,其性能好壞直接影響合成的效果。 本文按照反應器在流速均勻性、放置方位無限制性、密封性、化學穩(wěn)定性等方面的要求,從反應器結構和反應池形狀兩方面設計了一種新的微流體反應器。該反應器采用壓裝式結構,主要由基座、反應池板、玻片、橡膠圈和旋蓋組成。多種定位和固定設計使反應器的組裝簡單,使用方便。利用PDMS制作的反應池板上設有一個依據(jù)仿生學的原理設計的類似梭形的反應池。為了從理論上證明梭形反應池的流場分布均勻效果好于常規(guī)的菱形反應池,建立了張角為125度、135度和145度情況下的幾組模型,利用Fluent計算流體力學軟件進行仿真后,通過對仿真數(shù)據(jù)的分析,顯示了梭形反應池的均勻率R更大,均勻效果更好。進一步增大張角和改變進出口寬度進行仿真后,優(yōu)化了梭形反應池的設計。 將反應器按照最終設計方案加工裝配后,經(jīng)過手工實驗并用于光導原位合成系統(tǒng)中進行實際的曝光反應,最終得到了均一的與掩模一致的圖案,驗證了本設計的有效性,可以廣泛應用于各種基因芯片的合成系統(tǒng)中。
[Abstract]:As an analogue of DNA, peptide nucleic acid exhibits higher specificity and better stability when hybridized with corresponding DNA or RNA sequences. Therefore, peptide nucleic acid chips synthesized from peptide nucleic acid monomers as a new type of gene chip, have been widely used in various gene research in recent years. Among several synthesis methods of peptide nucleic acid chips, photoconductive in situ synthesis technology has the advantages of high synthesis speed, high chip density and low cost. The performance of microfluidic reactor, as the site of photoconductive in situ synthesis, directly affects the effect of synthesis. A new micro-fluid reactor was designed in this paper according to the requirements of the reactor in terms of the uniformity of the flow rate, the location of the reactor, the sealing and the chemical stability of the reactor, in terms of the structure of the reactor and the shape of the reactor cell. The reactor consists of a base, a reactor plate, a glass slide, a rubber ring and a cover. A variety of positioning and fixation designs make the reactor assembly simple and easy to use. There is a fusiform reaction cell designed according to the principle of bionics on the plate of reaction cell made by PDMS. In order to prove theoretically that the uniform distribution of flow field of fusiform reaction cell is better than that of conventional rhombic reactor, several models with tension angle of 125 degrees or 135 degrees and 145 degrees are established, and the simulation results are carried out by using Fluent computational fluid dynamics software. Through the analysis of the simulation data, it is shown that the uniform rate R of the fusiform reaction cell is larger and the uniformity effect is better. After further increasing the opening angle and changing the width of inlet and outlet, the design of fusiform reaction cell is optimized. After the reactor was processed and assembled according to the final design scheme, after manual experiments and used in the photoconductive in-situ synthesis system for the actual exposure reaction, a uniform pattern consistent with the mask was finally obtained, and the effectiveness of the design was verified. It can be widely used in the synthesis system of various gene chips.
【學位授予單位】：中南大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R318.6

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