本文選題：去細胞 + 胰腺　； 參考：《解剖學報》2014年04期

【摘要】：目的通過離體灌注化學去垢劑的方法制備大鼠胰腺去細胞天然生物支架,并對支架的完整性、生物相容性進行檢驗。方法健康成年SD大鼠30只,分別經膽管與血管兩條途徑灌注十二烷基硫酸鈉和曲亞通X-100等藥品洗脫,獲取胰腺去細胞生物支架。通過HE染色、掃描電子顯微鏡和透射電子顯微鏡等觀察細胞殘留于去細胞支架的生物學特性,分光光度計法鑒定殘留去垢劑量,酶聯(lián)免疫吸附劑測定法測定殘留支架蛋白含量及生長因子,并用腹壁與皮下包埋實驗檢驗其炎癥反應,MTT法測定支架細胞毒性,內皮細胞共培養(yǎng)測定支架生物相容性。結果 HE染色、掃描電子顯微鏡和透射電子顯微鏡觀察均表明去細胞支架無細胞殘留,細胞外支架連續(xù)性完好,脈管支架保存完整;藥物殘留小于規(guī)定標準;細胞外支架生長因子及支架蛋白存留量等指標上血管組均優(yōu)于膽管組;腹壁與皮下包埋實驗顯示,去細胞支架炎性反應較對照組明顯減輕;MTT法顯示無細胞毒性;內皮細胞共培養(yǎng)有黏附趨勢。結論使用兩種灌注法制備胰腺去細胞生物支架,均可將細胞去除徹底,支架生物相容性好。但就血管組細胞外支架保留完整性,生長因子保留更多,則血管灌注途徑優(yōu)于膽管灌注途徑。
[Abstract]:Aim to prepare the acellular natural scaffold of rat pancreas by in vitro infusion of chemical defouling agent, and to test the integrity and biocompatibility of the scaffold. Methods 30 healthy adult SD rats were perfused with drugs such as sodium dodecyl sulfate and triazone X-100 respectively through bile duct and blood vessel to obtain acellular pancreatic scaffold. He staining, scanning electron microscope and transmission electron microscope were used to observe the biological characteristics of cells remaining in the scaffold, and the residual defouling dose was identified by spectrophotometer. The contents of residual stents protein and growth factors were determined by Elisa. The cytotoxicity of stents was determined by MTT assay and the biocompatibility of stents was determined by co-culture of endothelial cells. Results HE staining, scanning electron microscopy and transmission electron microscopy showed that there were no cell residues in the acellular stents, the extracellular stents were intact, the vascular stents were intact, and the drug residues were less than the prescribed standard. The upper vascular group was superior to the bile duct group in the indexes of extracellular stent growth factor and stenting protein retention, and the inflammatory response of acellular stents in abdominal wall and subcutaneous embedding was significantly less than that in the control group, and no cytotoxicity was observed by MTT assay. The co-culture of endothelial cells has the tendency of adhesion. Conclusion the acellular scaffolds of pancreas prepared by two kinds of perfusion methods can remove the cells completely and have good biocompatibility. However, the vascular perfusion pathway is superior to the bile duct perfusion pathway for the integrity of extracellular stent and more growth factors in vascular group.
【作者單位】： 溫州醫(yī)科大學第一臨床醫(yī)學院;溫州醫(yī)科大學生物支架移植與免疫研究所;溫州醫(yī)科大學基礎醫(yī)學中心;
【基金】：國家級大學生創(chuàng)新創(chuàng)業(yè)訓練計劃資助項目(201310343006,201210343008) 浙江省自然科學基金資助項目(LY12H15002) 浙江省大學生科技創(chuàng)新活動計劃“新苗人才計劃”資助項目(2012R413004) 浙江省教育廳資助項目(Y201327980)
【分類號】：R318.08

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