本文選題：絲素蛋白 + 水凝膠　； 參考：《蘇州大學》2015年碩士論文

【摘要】：絲素蛋白水凝膠基于自身良好的生物相容性,在生物材料方面具有潛在的應(yīng)用。本文研究了三類表面活性劑,即甜菜堿類表面活性劑、多肽類表面活性劑和氨基酸類表面活性劑誘導(dǎo)絲素形成水凝膠,表征其結(jié)構(gòu)和性能,并探究其凝膠化機理。甜菜堿類表面活性劑能加快絲素蛋白凝膠化,其凝膠化時間與活性劑碳鏈長度無關(guān),而與表面張力有關(guān),表面張力出現(xiàn)最低點的活性劑成凝膠時間較短,其中十二烷基二甲基甜菜堿誘導(dǎo)絲素形成凝膠時間較短,在20 min左右。對所形成的水凝膠結(jié)構(gòu)和性能進行表征,并通過熒光測試其內(nèi)部結(jié)構(gòu)變化,這一過程中,在甜菜堿類表面活性劑的親疏水作用下,絲素蛋白分子鏈伸張開并重組,絲素蛋白構(gòu)象由無規(guī)線團向β-折疊結(jié)構(gòu)轉(zhuǎn)變,形成結(jié)構(gòu)均勻,孔徑較大的三維網(wǎng)絡(luò)結(jié)構(gòu)水凝膠,柔軟而富有彈性,具有一定的抑菌作用,可作為水凝膠醫(yī)用敷料,解決傳統(tǒng)創(chuàng)口貼帶來的諸多不便。多肽類表面活性劑枯草菌脂肽鈉誘導(dǎo)絲素蛋白形成水凝膠,所需枯草菌脂肽鈉濃度較低,37℃下1 g/L的枯草菌脂肽鈉即可誘導(dǎo)絲素在2 h內(nèi)形成水凝膠,且凝膠具有一定的細胞生物相容性。氨基酸類表面活性劑N-月桂酰肌氨酸鈉(SNS)能夠加速絲素蛋白形成水凝膠。SNS與絲素混合后,可通過溫度、SNS濃度和絲素濃度的調(diào)節(jié)來滿足所需膠凝時間。利用紅外光譜、X射線衍射、掃描電鏡、圓二色光譜和熒光光譜等對水凝膠的結(jié)構(gòu)、性能和凝膠機理進行探究。發(fā)現(xiàn)SNS誘導(dǎo)絲素蛋白分子鏈在親疏水力和靜電作用下展開重構(gòu),形成更多的結(jié)晶結(jié)構(gòu)交聯(lián)成水凝膠。SNS/SF水凝膠結(jié)構(gòu)穩(wěn)定,具有相對較高的強度和優(yōu)良的彈性,在適度應(yīng)力作用下變形可回復(fù),細胞生物相容性良好。SNS/SF水凝膠在組織修復(fù)方面具有巨大的潛力。
[Abstract]:Silk fibroin hydrogel has potential applications in biomaterials because of its good biocompatibility. In this paper, three kinds of surfactants, beet surfactants, polypeptide surfactants and amino acid surfactants, have been studied to induce silk fibroin to form hydrogels, to characterize their structure and properties, and to explore their gelation mechanism. Beet surfactants can accelerate the gelation of silk fibroin protein. The gelation time is not related to the length of the carbon chain of the surfactant, but to the surface tension. The gelation time of the surfactant with the lowest surface tension is shorter. The gelation time of silk fibroin induced by dodecyl dimethyl betaine was about 20 min. The structure and properties of the hydrogel were characterized, and its internal structure was measured by fluorescence. In this process, the silk fibroin molecular chain was stretched and recombined under the hydrophobic action of betaine surfactants. The conformation of silk fibroin is changed from random coil to 尾 -fold structure to form a three-dimensional hydrogel with uniform structure and large pore diameter. It is soft and elastic, has certain bacteriostatic effect, and can be used as hydrogel medical dressing. To solve the traditional band-paste brought many inconveniences. The polypeptide surfactant, sodium subtilis lipidopeptide, induced the formation of hydrogel of silk fibroin protein, and the concentration of sodium subtilis lipid peptide sodium was 1 g / L at 37 鈩，

本文編號：1829525