發(fā)布時間：2018-04-11 06:56

  本文選題：多孔鉭 + 生物相容性材料　； 參考：《解放軍醫(yī)學雜志》2014年06期

【摘要】：目的評價多孔鉭材料復合大鼠軟骨細胞的體外生長特性及功能變化。方法選取3周齡SD大鼠,分離關節(jié)軟骨細胞并進行體外擴增培養(yǎng),取生長狀態(tài)良好的第2代細胞,鑒定后以1×106/ml密度接種于多孔鉭上進行復合培養(yǎng)。采用倒置相差顯微鏡、掃描電鏡、透射電鏡觀察軟骨細胞在多孔鉭上的形態(tài)特點,比色法測定復合培養(yǎng)的軟骨細胞中糖胺多糖(GAG)的含量。結果經(jīng)Ⅱ型膠原免疫細胞化學染色、甲苯胺藍染色及番紅O染色鑒定所獲細胞為軟骨細胞。倒置相差顯微鏡觀察可見多孔鉭邊緣及培養(yǎng)板底部黏附大量軟骨細胞;掃描電鏡觀察可見軟骨細胞在多孔鉭表面及孔隙內黏附、伸展、增殖,并分泌細胞外基質;透射電鏡觀察顯示軟骨細胞內超微結構大小及形態(tài)無明顯改變;比色法檢測顯示軟骨細胞在多孔鉭支架上可持續(xù)分泌GAG。結論多孔鉭材料與軟骨細胞體外共培養(yǎng)顯示出良好的生物相容性,適合作為軟骨組織工程的支架材料。
[Abstract]:Objective to evaluate the growth characteristics and functional changes of porous tantalum composite chondrocytes in vitro.Methods the articular chondrocytes were isolated from 3-week-old SD rats and cultured in vitro. The second generation cells in good growth state were identified and cultured on porous tantalum with a density of 1 脳 106/ml.The morphologic characteristics of chondrocytes on porous tantalum were observed by inverted phase contrast microscope, scanning electron microscope and transmission electron microscope. The content of glycosaminoglycan (GAG) in chondrocytes was determined by colorimetry.Results the chondrocytes were identified as chondrocytes by immunocytochemical staining of type 鈪，

本文編號：1734897