本文選題：殼聚糖-聚己內(nèi)酯　切入點：殼聚糖微球　出處：《華中科技大學(xué)》2013年碩士論文

【摘要】：采用基團保護方法合成了殼聚糖-聚己內(nèi)酯共聚物。在優(yōu)化條件下，聚己內(nèi)酯側(cè)鏈的接枝率可達到約70%。對殼聚糖-聚己內(nèi)酯進行了表征。合成產(chǎn)物的化學(xué)結(jié)構(gòu)和物理性質(zhì)通過紅外光譜，核磁共振光譜進行定性。 以京尼平作為交聯(lián)劑，通過乳化交聯(lián)技術(shù)制備了載TGF-β1因子的殼聚糖微球。利用掃描電子顯微鏡觀察了微球的形態(tài)和尺寸，通過圖像分析系統(tǒng)測定了微球的粒徑分布和平均直徑，篩選出粒徑低于45μm微球用于支架/微球復(fù)合物制備。通過調(diào)節(jié)固化過程中交聯(lián)劑的用量，可以使微球的包封率達到80%以上。 將篩選出的載TGF-β1微球復(fù)合到殼聚糖-聚己內(nèi)酯共聚物疏松多孔支架中，研究發(fā)現(xiàn)通過改變CH-PCLS的組成比例和支架的結(jié)構(gòu)參數(shù)等可有效控制支架的機械強度和因子的釋放。聚己內(nèi)酯的含量控制在42wt.%左右可以得到較好的效果。通過體外模擬釋放實驗得出高于80%孔隙率的支架材料、初始載藥量為3ng(TGF-β1)/mg(支架重量)的支架可以實現(xiàn)TGF-β1因子的控制釋放超過四周，，且不具有明顯的突釋性。經(jīng)膨脹性質(zhì)和力學(xué)性質(zhì)測量得出這種類型的微球/殼聚糖-聚己內(nèi)酯支架復(fù)合體在濕態(tài)下具有較好的好的壓縮力學(xué)性能。
[Abstract]:Chitosan-polycaprolactone copolymers were synthesized by group protection method. The graft rate of the side chain of polycaprolactone was about 70%. The chemical structure and physical properties of the synthesized products were characterized by IR and NMR spectra. Chitosan microspheres loaded with TGF- 尾 1 were prepared by emulsification and crosslinking with genipine as crosslinking agent. The morphology and size of the microspheres were observed by scanning electron microscope (SEM). The particle size distribution and average diameter of the microspheres were measured by image analysis system. The microspheres with diameter less than 45 渭 m were selected for the preparation of scaffold / microsphere composites. The amount of crosslinking agent was adjusted during the curing process. The encapsulation efficiency of the microspheres can reach more than 80%. The selected TGF- 尾 1 microspheres were mixed into the porous scaffolds of chitosan / polycaprolactone copolymers. It is found that the mechanical strength and factor release of the scaffold can be effectively controlled by changing the composition ratio of CH-PCLS and the structural parameters of the scaffold, and the content of polycaprolactone can be controlled at about 42 wt.%. Pseudo-release experiments show that scaffold materials with a porosity of more than 80%, A stent with an initial dose of 3ng-1 TGF- 尾 1 / mg (stent weight) can control the release of TGF- 尾 1 factor for more than four weeks. It was found that the microspheres / chitosan-polycaprolactone scaffolds had good compressive mechanical properties under wet conditions.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R318.08

【參考文獻】

相關(guān)期刊論文 前2條

1 楊艾玲;;殼聚糖的研究進展[J];化工中間體;2009年10期

2 厲孟;劉旭東;劉興炎;;京尼平與戊二醛交聯(lián)明膠微球的性能比較[J];中國修復(fù)重建外科雜志;2009年01期

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