本文關鍵詞： 纖維膠原類 軟骨細胞 軟骨 組織工程 生物材料 軟骨生物材料 微纖維膠原 國家自然科學基金　出處：《中國組織工程研究》2017年22期 　論文類型：期刊論文

【摘要】：背景:尋找理想的組織工程支架材料,仍然是軟骨組織工程學的重要課題之一。目的:探討微纖維膠原應用于軟骨組織工程支架的可行性。方法:手術切取兔耳軟骨,提取原代軟骨細胞,并進行擴增培養(yǎng)。將海綿狀微纖維膠原用無菌刀片切割成小方塊,取第2代軟骨細胞混懸液接種于微纖維膠原支架材料上。體外培養(yǎng)1周后,植入裸鼠體內(nèi),觀察8周后取材,進行大體、組織學、免疫組織化學等方法觀察。結果與結論:(1)在細胞接種過程中,濕水之后的微纖維膠原支架無明顯縮水,仍然能夠保持其立體形態(tài);(2)體外培養(yǎng)1周以及植入體內(nèi)8周后,無明顯體積和形態(tài)變化;(3)裸鼠體內(nèi)培養(yǎng)8周后形成白色半透明且富有彈性的成熟軟骨塊;(4)組織學觀察可見所構建的軟骨組織成熟,基質(zhì)分泌旺盛,組織內(nèi)仍可見未完全降解的微纖維膠原材料;(5)結果表明,微纖維膠原蛋白是一種良好的軟骨組織工程支架材料。
[Abstract]:Background: searching for ideal scaffold materials for tissue engineering is still one of the important topics in cartilage tissue engineering. Objective: to explore the feasibility of the application of microfiber collagen in cartilage tissue engineering scaffolds. The primary chondrocytes were extracted and cultured. The spongy microfibrous collagen was cut into small cubes with aseptic blades, and the second passage chondrocytes were mixed with the scaffold of microfiber collagen. After 1 week of culture in vitro, the chondrocytes were cultured for 1 week. Results and conclusion: during cell inoculation, microfiber collagen scaffolds did not shrink significantly after wetting water, and the microfibrillar collagen scaffolds did not shrink after wetting water, and the microfibrillar collagen scaffolds were taken from nude mice after 8 weeks of observation, and were observed by gross, histological and immunohistochemical methods. Still able to maintain its stereomorphology for 1 week in vitro and 8 weeks after implantation. After 8 weeks of culture in nude mice, white translucent and elastic mature chondrocytes were formed. Histological observation showed that the chondrocytes were mature and exuberant in matrix secretion. The results showed that microfibrillar collagen was a good scaffold for cartilage tissue engineering.
【作者單位】： 廣州醫(yī)科大學附屬口腔醫(yī)院 廣州口腔疾病研究所 口腔醫(yī)學重點實驗室;解放軍第306醫(yī)院耳鼻咽喉頭頸外科;
【基金】：國家自然科學基金青年科學基金項目(81401609)~~
【分類號】：R318.08
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本文編號：1550227