本文關(guān)鍵詞：載微球殼聚糖引導(dǎo)骨組織再生膜的制備及力學(xué)性能研究　出處：《太原理工大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 納米羥基磷灰石 殼聚糖 復(fù)合微球 原位仿生 引導(dǎo)骨組織再生膜

【摘要】：骨缺損的再生修復(fù)已成為目前再生醫(yī)學(xué)研究的重要課題。大量研究表明引導(dǎo)骨組織再生膜(GBR)技術(shù)是一種治療骨缺損的有效方法。臨床上要求GBR膜需具有良好生物相容性、生物活性和骨傳導(dǎo)性,并具有一定的力學(xué)強度以抵抗周圍纖維組織的侵入。 本研究采用具有良好生物相容性、生物降解性的殼聚糖(CS)制備了GBR膜,為改善GBR膜的力學(xué)強度及成骨活性,在GBR膜中引入納米羥基磷灰石(nHA)與CS復(fù)合微球,期望該膜在起到屏障作用的同時,兼具良好的骨傳導(dǎo)作用,促進骨缺損的再生修復(fù)。 前期研究大多采用結(jié)合乳化原理的共混法來制備復(fù)合微球,本研究采用原位仿生技術(shù)制備nHA/CS復(fù)合微球,充分利用無機納米粒子(nHA)表面活性較高和比表面積較大的優(yōu)點,將其均勻分散在高分子基質(zhì)(CS)中與活性基團產(chǎn)生化學(xué)鍵結(jié)合,獲得各方面性能理想的復(fù)合微球,并與使用共混原理制備的復(fù)合微球進行了比較和分析研究。利用掃描電鏡(SEM)、 X射線能譜(EDS)、X射線衍射(XRD)、紅外(FTIR)和激光粒度儀等手段對不同微球的理化性能進行表征。結(jié)果表明：相比共混法,原位仿生制備的nHA/CS復(fù)合微球形態(tài)圓整均勻,分散性好,粒徑分布較窄,平均粒徑為8.62μm, nHA晶體均勻分布在微球內(nèi)部及表面并與CS基質(zhì)以化學(xué)鍵結(jié)合。 設(shè)計并制備分別載有純CS微球、原位仿生nHA/CS復(fù)合微球以及共混nHA/CS復(fù)合微球的新型GBR膜。通過SEM對比觀察三種引導(dǎo)骨組織再生膜表面或斷面的形貌及結(jié)構(gòu),研究不同微球在GBR膜內(nèi)及表面分布情況,并采用萬能電子試驗機對三種膜進行力學(xué)性能測試。結(jié)果顯示,引入不同微球后GBR膜表面有顆粒狀突起,微球均包覆在GBR膜內(nèi)部。斷面結(jié)構(gòu)顯示,CS微球與CS基質(zhì)之間有明顯空隙,共混法nHA/CS復(fù)合微球中nHA團聚形成較大團簇,不利于復(fù)合微球與CS基質(zhì)的結(jié)合,而原位法nHA/CS復(fù)合微球與CS基質(zhì)結(jié)合較為緊密,微球表面nHA晶體能有效連接微球與CS基質(zhì)。拉伸實驗表明,相比純CS膜,引入不同微球后,斷裂伸長率均有所下降,其中原位法復(fù)合微球斷裂伸長率最大,達到5.61±0.95%。相比純CS膜,引入CS微球后,其彈性模量和強度極限有所下降,而共混法與原位法復(fù)合微球組均有所升高,原位法彈性模量和強度極限達到最大,分別為766.272±20.675和43.318±0.951MPa。
[Abstract]:The regeneration and repair of bone defects has become an important subject in regenerative medicine. A large number of studies have shown that the bone tissue regeneration membrane can be guided by GBR. Technology is an effective method for the treatment of bone defect. It is necessary to have good biocompatibility of GBR membrane in clinic. Biological activity, bone conductivity, and a certain degree of mechanical strength to resist the invasion of surrounding fibrous tissue. In order to improve the mechanical strength and osteogenic activity of GBR membrane, GBR membrane was prepared by chitosan with good biocompatibility and biodegradability. Nano-hydroxyapatite (HA) and CS composite microspheres were introduced into the GBR membrane. It is expected that the composite microspheres not only act as a barrier but also have good bone conduction and promote the regeneration and repair of bone defects. In previous studies, most of the composite microspheres were prepared by blending with emulsification principle. In this study, nHA/CS composite microspheres were prepared by in situ bionic technology. Taking full advantage of the advantages of high surface activity and large specific surface area of inorganic nano-particles (NHAs), they were uniformly dispersed in the polymer matrix (CSS) to form chemical bonds with active groups. The composite microspheres with ideal properties in various aspects were obtained and compared with the composite microspheres prepared by using the blending principle. The scanning electron microscopy (SEM) and X-ray energy dispersive spectroscopy (EDS) were used to prepare the composite microspheres. The physicochemical properties of different microspheres were characterized by X-ray diffraction (XRD), FTIR (FTIR) and laser particle size analyzer. The nHA/CS composite microspheres prepared by in situ bionics have the advantages of uniform morphology, good dispersion, narrow particle size distribution and average particle size of 8.62 渭 m. The nHA crystal is uniformly distributed inside and on the surface of the microsphere and binds to the CS substrate by chemical bond. The pure CS microspheres were designed and prepared. In situ biomimetic nHA/CS composite microspheres and blend nHA/CS composite microspheres of a new GBR membrane. The morphology and structure of three kinds of guided bone tissue regeneration membrane surface or section were observed by SEM. The distribution of different microspheres in and on the surface of GBR film was studied, and the mechanical properties of three kinds of membranes were tested by universal electronic testing machine. The results showed that there were granular protrusions on the surface of GBR film after the introduction of different microspheres. The cross section structure showed that there was a clear void between CS microspheres and CS matrix, and nHA agglomerated into large clusters in nHA/CS composite microspheres by blending. It is not conducive to the binding of composite microspheres to CS matrix, while in situ nHA/CS composite microspheres bind more closely to CS matrix. The nHA crystal on the surface of the microspheres can effectively connect the microspheres to the CS substrate. The tensile experiments show that the elongation at break decreases with the addition of different microspheres compared with the pure CS films. The elongation at break of in-situ composite microspheres reached 5.61 鹵0.95.Compared with the pure CS film, the elastic modulus and the strength limit of the composite microspheres decreased after the introduction of CS microspheres. The elastic modulus and strength limit of in-situ method reached the maximum of 766.272 鹵20.675 and 43.318 鹵0.951 MPa, respectively.
【學(xué)位授予單位】：太原理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R318.08

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