【摘要】：背景:以往關(guān)于器官移植免疫抑制和抗排斥反應的研究,多關(guān)注T淋巴細胞介導的免疫反應及免疫抑制劑對T淋巴細胞的作用,樹突狀細胞作用尚不清楚,且對于免疫抑制劑對樹突狀細胞影響的表現(xiàn)及機制亦不盡相同。目的:比較不同免疫抑制劑對樹突狀細胞共刺激分子表達及功能的影響,探討其免疫抑制作用機制。方法:在誘導C57BL/6小鼠骨髓細胞定向分化為樹突狀細胞時分別加入20μg/L雷帕霉素、0.04 mg/L霉酚酸酯、10μg/L他克莫司和1 mg/L環(huán)孢霉素A。結(jié)果與結(jié)論:流式細胞儀檢測結(jié)果顯示,各組CD40表達為:雷帕霉素他克莫司環(huán)孢霉素A霉酚酸酯(P0.01);CD86表達分別為:雷帕霉素他克莫司環(huán)孢霉素A=霉酚酸酯(P0.01);CD80、CD11c及主要組織相容性復合體Ⅱ的表達各組差異無顯著性意義(P0.05)。單向混合淋巴細胞反應結(jié)果顯示,各組樹突狀細胞共刺激T細胞增殖能力表現(xiàn)為:雷帕霉素霉酚酸酯他克莫司=環(huán)孢霉素A(P0.05)。結(jié)果證實,雷帕霉素、他克莫司、環(huán)孢霉素A、霉酚酸酯均通過抑制樹突狀細胞共刺激分子CD40及CD86表達而發(fā)揮免疫抑制作用,以雷帕霉素最為顯著;雖然以上藥物對樹突狀細胞抗原遞呈能力主要組織相容性復合體Ⅱ的表達均無明顯抑制性,但均能抑制樹突狀細胞刺激T細胞增殖的能力,雷帕霉素的抑增殖能力最強。
[Abstract]:Background: previous studies on immunosuppression and anti-rejection in organ transplantation have focused on the immune response mediated by T lymphocytes and the effects of immunosuppressive agents on T lymphocytes. The effect of dendritic cells on T lymphocytes is still unclear. The effects and mechanisms of immunosuppressive agents on dendritic cells were also different. Aim: to compare the effects of different immunosuppressive agents on the expression and function of costimulatory molecules of dendritic cells and to explore the mechanism of immunosuppressive effect. Methods: 20 渭 g / L rapamycin, 0.04 mg/L mycophenolate mofetil, 10 渭 g / L tacrolimus and 1 mg/L cyclosporine A. Results and conclusion: the results of flow cytometry showed that the expression of CD40 in each group was: rapamycin tacrolimycin A mycophenolate mofetil (P0.01); The expression of CD86 was: rapamycin tacrosporine A = mycophenolate mofetil (P0.01), but there was no significant difference in the expression of CD80,CD11c and major histocompatibility complex 鈪，

本文編號：2468579