發(fā)布時間：2018-11-02 17:18

【摘要】： 目的 研究軟骨源性形態(tài)發(fā)生蛋白-2(CDMP-2)對大鼠骨髓間充質(zhì)干細胞(BMSCs)向軟骨分化的作用。 方法 體外培養(yǎng)大鼠骨髓間充質(zhì)干細胞(BMSCs),貼壁細胞傳代,取第3代細胞,對照組(A組)以L-DMEM無血清培養(yǎng)液培養(yǎng),實驗組(B、C、D組)以L-DMEM無血清培養(yǎng)液培養(yǎng),分別加入CDMP-2(100 ng/ml)、TGF-β1(10 ng/ml)和CDMP-2(100ng/ml)+TGF-β1(10 ng/ml),倒置相差顯微鏡觀察細胞形態(tài),14天后終止培養(yǎng),行甲苯胺藍染色,免疫組織化學(xué)染色和Western blot檢測軟骨特異性Ⅱ型膠原的表達。 結(jié)果 誘導(dǎo)14天后可見BMSCs形態(tài)由梭形向軟骨細胞的多邊形轉(zhuǎn)變。甲苯胺藍染色示糖胺聚糖(GAGs)均勻分布于基質(zhì)中,免疫組織化學(xué)染色和Western blot示實驗組Ⅱ型膠原表達陽性,且CDMP-2+TGF-β1組細胞表達Ⅱ型膠原水平高于單獨給予CDMP-2、TGF-β1組(P＜0.05);對照組未見陽性表達。 結(jié)論 CDMP-2能夠在體外定向誘導(dǎo)大鼠骨髓間充質(zhì)干細胞(BMSCs)向軟骨方向分化;與TGF-β1相比,CDMP-2誘導(dǎo)能力更強;CDMP-2與TGF-β1在誘導(dǎo)BMSCs向軟骨分化時具有協(xié)同作用,兩者合用成軟骨的效應(yīng)最大。
[Abstract]:Objective to study the effect of chondrogenic morphogenetic protein-2 (CDMP-2) on the differentiation of rat bone marrow mesenchymal stem cells (BMSCs) into cartilage. Methods (BMSCs), adherent cells of rat bone marrow mesenchymal stem cells were cultured in vitro. The third passage cells were obtained. The control group (A group) was cultured in L-DMEM serum-free medium. In group D, CDMP-2 (100 ng/ml), TGF- 尾 1 (10 ng/ml) and CDMP-2 (100ng/ml) TGF- 尾 1 (10 ng/ml) were added to L-DMEM serum-free medium to observe cell morphology. After 14 days, the culture was terminated, toluidine blue staining, immunohistochemical staining and Western blot were used to detect the expression of cartilage specific type 鈪，

本文編號：2306441