【摘要】：目的探索新佐劑CpG ODN在重組乙肝疫苗中的免疫效應及吸附狀態(tài)。方法 CpG ODN佐劑與重組乙肝疫苗混合免疫小鼠,收集小鼠血清檢測。通過免疫小鼠后的體液和細胞免疫應答水平來評價雙佐劑乙肝疫苗對重組乙肝疫苗的增強作用。CpG ODN佐劑與乙肝疫苗在不同溫度條件下混合吸附,計算在不同吸附時間下CpG ODN佐劑在乙肝疫苗中的吸附率。結果CpG ODN佐劑可以提高重組乙肝疫苗在體液及細胞中的免疫效果。CpG ODN佐劑在室溫條件下1 h即可在乙肝疫苗中達到吸附平衡,于2~8℃放置5 d的條件下其吸附率約在50%左右。結論 CpG ODN能夠增加乙肝疫苗的免疫應答,其免疫效果可提高5倍以上。
[Abstract]:Objective to investigate the immune effect and adsorption state of new adjuvant CpG ODN in recombinant hepatitis B vaccine. Methods mice were immunized with CpG ODN adjuvant and recombinant hepatitis B vaccine. The effects of double adjuvant hepatitis B vaccine on the enhancement of recombinant hepatitis B vaccine were evaluated by the humoral and cellular immune response levels of mice immunized. The. CpG ODN adjuvant and hepatitis B vaccine were mixed and adsorbed at different temperatures. The adsorption rate of CpG ODN adjuvant in hepatitis B vaccine was calculated at different adsorption time. Results CpG ODN adjuvant could improve the immune effect of recombinant hepatitis B vaccine in body fluid and cells. The adsorption equilibrium of. CpG ODN adjuvant in hepatitis B vaccine could be reached at room temperature for 1 h, and the adsorption rate of. CpG ODN adjuvant was about 50% at 2 ~ 8 鈩，

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