【摘要】： 目的:用Wistar大鼠建立動脈粥樣硬化(atherosclerosis,As)合并慢性牙周炎(chronic periodontitis,CP)動物模型,以探討冠心病與慢性牙周炎可能的相關性。方法:60只大鼠隨機分為四組,每組15只:A正常對照組,B慢性牙周炎組,C動脈粥樣硬化組,D慢性牙周炎合并動脈粥樣硬化組。各組接受相應的建模處理。實驗周期為12周。每20天檢查牙周指標并測定體質量。建模結束后,檢測血脂水平及ELISA法檢測血清白介素6(interleukin-6,IL-6)、C反應蛋白(C-reactive protein,CRP)水平。組織病理學觀察各組實驗牙牙周組織及動脈血管病理改變。結果:牙周檢查發(fā)現(xiàn),B、D組附著喪失水平(attachment loss,AL)、齦溝出血指數(shù)(sulcular bleeding index,SBI)較A、C組有明顯增加,且差別有統(tǒng)計學意義(P0.05)。C、D組TC、TG、LDL水平較A、B組明顯升高(P0.05),D組血清IL-6、CRP水平均高于其他組(P0.05)。組織病理檢查發(fā)現(xiàn)B組動脈血管中膜可見不均勻增厚。C、D組主動脈及胸、腹主動脈形成動脈粥樣硬化病變。結論:結合牙周檢查、血脂檢測和組織病理學檢查綜合判定,初步成功建立大鼠CP合并As動物模型。對各組IL-6、CRP水平的分析,CP可能通過炎癥因子的作用影響As的發(fā)生發(fā)展。
[Abstract]:Aim: to establish an animal model of atherosclerosis (atherosclerosis,As) with chronic periodontitis (chronic periodontitis,CP) in Wistar rats and to explore the possible correlation between coronary heart disease and chronic periodontitis. Methods: sixty rats were randomly divided into four groups, 15 rats in each group: a control group, B chronic periodontitis group, C atherosclerosis group, D chronic periodontitis combined with atherosclerosis group. Each group was treated with corresponding modeling. The experimental period was 12 weeks. Periodontal indexes were examined and body mass was measured every 20 days. After modeling, serum lipid level and serum interleukin 6 (interleukin-6,IL-6), C reactive protein) (C-reactive protein,CRP) level were detected by ELISA method. Histopathological changes of periodontal tissues and arterial vessels of experimental teeth were observed. Results: periodontal examination showed that the level of attachment loss (attachment loss,AL) and gingival sulcus bleeding index (sulcular bleeding index,SBI) in group B were significantly higher than those in group A (P 0.05), and the TC,TG,LDL level in group C was significantly higher than that in group A (P0.05). The level of serum IL-6,CRP in group C was significantly higher than that in group A (P0.05). Histopathological examination showed that there was an uneven thickening of the medial membrane of the arteries in group B. Atherosclerosis was found in the aorta, thoracic and abdominal aorta in group C and D. Conclusion: combined with periodontal examination, blood lipid detection and histopathological examination, the animal model of CP combined with As was established successfully. The level of IL-6,CRP in each group was analyzed. CP may influence the development of As through the action of inflammatory factors.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2009
【分類號】：R781.4;R543.5;R-332

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