發(fā)布時間：2018-10-10 15:39

【摘要】：目的探究膽石六號湯劑對膽色素結(jié)石豚鼠膽囊腫瘤壞死因子-α(TNF-α)及核轉(zhuǎn)錄因子(NF-κB)表達(dá)的影響。方法將雌性豚鼠40只隨機(jī)分成3組,空白組10只,模型組15只,實(shí)驗(yàn)組15只�？瞻捉M除外,其余2組均采用飲食誘導(dǎo)法,建立膽色素結(jié)石模型。實(shí)驗(yàn)組,灌胃膽石六號湯劑7.7g·kg-1·d-1,其余2組均灌胃用同體積的0.9%氯化鈉。30 d后,觀察各組豚鼠成石率,用免疫組織化學(xué)技術(shù)檢測膽囊TNF-α及NF-κB蛋白表達(dá)和膽囊病理組織的改變情況。結(jié)果模型組成石率為90.62%,明顯高于空白組26.76%,表明造模成功;實(shí)驗(yàn)組成石率為11.00%,明顯低于模型組90.62%,可見藥物具有抗膽結(jié)石的作用。與空白組比較,模型組的TNF-α、NF-κB蛋白表達(dá)量明顯增加(P0.01)。與模型組比較,實(shí)驗(yàn)組的TNF-α、NF-κB蛋白表達(dá)量明顯較少(P0.05)。結(jié)論膽石六號湯劑可以明顯減少膽色素結(jié)石豚鼠的成石率,這可能與藥物降低膽色素結(jié)石豚鼠膽組織TNF-α、NF-κB蛋白的表達(dá)有關(guān)。
[Abstract]:Objective to investigate the effect of Ganshi No.6 decoction on the expression of tumor necrosis factor- 偽 (TNF- 偽) and nuclear transcription factor (NF- 魏 B) in gallbladder of pigmented gallstone guinea pigs. Methods 40 female guinea pigs were randomly divided into 3 groups: blank group (n = 10), model group (n = 15) and experimental group (n = 15). Except the blank group, the other two groups were induced by diet to establish the model of pigment gallstone. In the experimental group, 7. 7 g kg-1 d-1 of gavage gallstone no. 6 decoction, and 0. 9% sodium chloride in the other two groups were given intragastrically. 30 days later, the lithogenesis rate of guinea pigs in each group was observed, and the expression of TNF- 偽 and NF- 魏 B protein in gallbladder and the changes of gallbladder pathological tissue were detected by immunohistochemical technique. Results the rate of model stone composition was 90.62, which was significantly higher than that of blank group 26.76, which indicated that the model was successful, and the experimental composition stone rate was 11.00, which was significantly lower than that of model group 90.62. It can be seen that the drug has the effect of anti-gallstone. Compared with the blank group, the expression of TNF- 偽 and NF- 魏 B protein in the model group was significantly increased (P0.01). Compared with the model group, the expression of TNF- 偽 and NF- 魏 B protein in the experimental group was significantly lower (P0.05). Conclusion the gallstone formation rate of the guinea pigs with gallbladder pigment stone can be significantly reduced by the decoction. This may be related to the decrease of the expression of TNF- 偽 and NF- 魏 B protein in the gallbladder tissue of the guinea pigs with gallbladder pigment stone.
【作者單位】： 遵義醫(yī)學(xué)院附屬醫(yī)院藥劑科;遵義醫(yī)學(xué)院藥學(xué)院;
【基金】：貴州省教育廳自然科學(xué)基金資助項(xiàng)目[黔教合K字(2012)054號] 貴州省科學(xué)技術(shù)基金資助項(xiàng)目[黔科合J字(2008)215號]
【分類號】：R285.5;R-332

【參考文獻(xiàn)】

相關(guān)期刊論文 前8條

1 彭定偉,劉丹,齊剛;利膽中藥的研究進(jìn)展[J];武警醫(yī)學(xué)院學(xué)報(bào);2005年01期

2 章學(xué)林;梁曉強(qiáng);顧宏剛;馬恩偉;林天碧;孫遜;張靜U，

本文編號：2262331