本文選題：間充質(zhì)干細(xì)胞 + 臍帶��； 參考：《北京協(xié)和醫(yī)學(xué)院》2009年碩士論文

【摘要】：研究背景：間充質(zhì)干細(xì)胞(mesenchymal stem cells, MSCs)是一種具有自我更新能力和多向分化潛能的成體干細(xì)胞,它來(lái)源廣泛、取材方便,同時(shí)具有造血支持及免疫調(diào)節(jié)功能,這使其成為有前途的臨床使用種子細(xì)胞。成體骨髓來(lái)源的間充質(zhì)干細(xì)胞是研究較早、較深入的間充質(zhì)干細(xì)胞,但是其取材侵襲性強(qiáng),對(duì)供者傷害較大：細(xì)胞的數(shù)量、增殖和分化能力隨供者年齡增長(zhǎng)而顯著下降；容易被病毒感染,這些因素限制了骨髓來(lái)源間充質(zhì)干細(xì)胞的應(yīng)用。大量研究已證實(shí),間充質(zhì)干細(xì)胞還存在于多種組織中,本實(shí)驗(yàn)采用了臍帶及脂肪來(lái)源的間充質(zhì)干細(xì)胞與成人骨髓來(lái)源的間充質(zhì)干細(xì)胞進(jìn)行生物學(xué)比較,期望獲得更佳的種子細(xì)胞來(lái)源。 目的：分離培養(yǎng)成人骨髓、脂肪和臍帶來(lái)源的間充質(zhì)干細(xì)胞,比較三種不同來(lái)源間充質(zhì)干細(xì)胞的形態(tài)特征、增殖能力、表面標(biāo)志及誘導(dǎo)分化能力,為間充質(zhì)干細(xì)胞臨床應(yīng)用選擇細(xì)胞來(lái)源提供實(shí)驗(yàn)依據(jù)和理論依據(jù)。 方法：在本研究中我們利用直接貼壁培養(yǎng)法分離培養(yǎng)成人骨髓間充質(zhì)干細(xì)胞,利用酶消化法分離培養(yǎng)脂肪和臍帶來(lái)源的間充質(zhì)干細(xì)胞,并通過(guò)貼壁培養(yǎng)的方法進(jìn)一步純化獲得三種間充質(zhì)干細(xì)胞。使用顯微鏡技術(shù)比較觀察三種來(lái)源間充質(zhì)干細(xì)胞的形態(tài)特征。用MTT法檢測(cè)P3代的三種間充質(zhì)干細(xì)胞的增殖能力。通過(guò)體外連續(xù)傳代試驗(yàn)研究其體外傳代能力。用流式細(xì)胞學(xué)方法檢測(cè)三種P3/P4代間充質(zhì)干細(xì)胞的表面標(biāo)記。體外誘導(dǎo)三種間充質(zhì)干細(xì)胞向脂肪和成骨細(xì)胞分化,通過(guò)油紅0和von-Kossa染色鑒定比較分化結(jié)果。 結(jié)果：三種間充質(zhì)干細(xì)胞體外培養(yǎng)時(shí)呈長(zhǎng)梭型、旋渦狀、貼壁生長(zhǎng),與臍帶來(lái)源的間充質(zhì)干細(xì)胞相比,脂肪和骨髓來(lái)源的間充質(zhì)干細(xì)胞體積較大。通過(guò)生長(zhǎng)曲線分析,計(jì)算倍增時(shí)間發(fā)現(xiàn)三種間充質(zhì)干細(xì)胞的增殖能力由強(qiáng)至弱依次為臍帶來(lái)源間充質(zhì)干細(xì)胞、脂肪來(lái)源間充質(zhì)干細(xì)胞、骨髓來(lái)源間充質(zhì)干細(xì)胞。通過(guò)體外長(zhǎng)期傳代實(shí)驗(yàn),我們發(fā)現(xiàn)臍帶來(lái)源間充質(zhì)干細(xì)胞的傳代能力最強(qiáng),而脂肪來(lái)源的間充質(zhì)干細(xì)胞次之,傳代能力最差的是骨髓來(lái)源的間充質(zhì)干細(xì)胞。三種間充質(zhì)干細(xì)胞均表達(dá)CD90,CD105 (SH2), CD73 (SH3/4), CD166, CD29;不表達(dá)CD31、CD133、CD19、CDllb、CD34、CD45和HLA-DR (HLA-Ⅱ)。此外,三種間充質(zhì)干細(xì)胞經(jīng)油紅0染色證實(shí)均可向成脂細(xì)胞分化；von Kossa染色證實(shí)均可向成骨細(xì)胞分化。 結(jié)論：我們成功從成人骨髓、脂肪和臍帶組織中分離并培養(yǎng)出間充質(zhì)干細(xì)胞,這三種不同來(lái)源的間充質(zhì)干細(xì)胞的形態(tài)、增殖傳代及誘導(dǎo)分化等生物學(xué)特征存在一定差異,而表面標(biāo)記基本相同。 研究背景：端粒是位于真核細(xì)胞染色體末端的特殊結(jié)構(gòu),由端粒DNA和端粒蛋白質(zhì)組成,其對(duì)維持染色體的穩(wěn)定和完全有重要作用。一般情況下動(dòng)物細(xì)胞染色體的端粒隨著細(xì)胞分裂而縮短,當(dāng)縮短到某種程度時(shí)細(xì)胞將停止增殖并調(diào)亡。端粒酶在一般細(xì)胞中不表達(dá),只在生殖細(xì)胞、干細(xì)胞和腫瘤細(xì)胞中表達(dá),而在間充質(zhì)干細(xì)胞中端粒酶的表達(dá)情況研究結(jié)果不統(tǒng)一。脂肪源間充質(zhì)干細(xì)胞是基礎(chǔ)實(shí)驗(yàn)研究和臨床應(yīng)用較多的間充質(zhì)干細(xì)胞之一,本研究測(cè)定脂肪源間充質(zhì)干細(xì)胞的端粒酶活性,為其今后的實(shí)驗(yàn)研究及臨床應(yīng)用提供更詳盡的實(shí)驗(yàn)依據(jù)。 目的：分離鑒定成人脂肪來(lái)源的間充質(zhì)干細(xì)胞,測(cè)定成人脂肪來(lái)源的間充質(zhì)干細(xì)胞是否表達(dá)端粒酶活性。 方法：按國(guó)際細(xì)胞治療協(xié)會(huì)(the International Society for Cellular Therapy, ISCT)提出的標(biāo)準(zhǔn)鑒定從脂肪中分離、培養(yǎng)得到間充質(zhì)干細(xì)胞。體外誘導(dǎo)成人脂肪來(lái)源間充質(zhì)干細(xì)胞向成脂和成骨細(xì)胞分化,通過(guò)油紅0和von-Kossa染色鑒定。采用逆轉(zhuǎn)錄PCR和端粒酶重復(fù)序列擴(kuò)增法-酶鏈免疫吸附法(TRAP-ELISA法),檢測(cè)不同供者來(lái)源和不同傳代次數(shù)的脂肪源間充質(zhì)干細(xì)胞是否表達(dá)端粒酶活性。 結(jié)果：研究發(fā)現(xiàn)用我們的方法分離得到的脂肪源間充質(zhì)干細(xì)胞符合國(guó)際細(xì)胞治療協(xié)會(huì)提出的標(biāo)準(zhǔn)。不同供者來(lái)源和不同傳代次數(shù)的脂肪源間充質(zhì)干細(xì)胞均不表達(dá)端粒酶mRNA,也無(wú)端粒酶活性。 結(jié)論：成人脂肪源間充質(zhì)干細(xì)胞不表達(dá)端粒酶活性。
[Abstract]:Background: mesenchymal stem cells (MSCs) is a kind of adult stem cells with self-renewal ability and multidirectional differentiation potential. It has a wide range of sources, convenient to take materials, and also has hematopoiesis support and immunomodulatory function. This makes it a promising seedbed using seed cells. The mesenchymal stem cells from adult bone marrow stem cells are dry and fine. Cell is an early and deeper mesenchymal stem cell, but it has strong invasiveness and great damage to the donor: the number of cells, proliferation and differentiation ability decrease significantly with the age of the donor; it is easy to be infected by the virus. These factors restrict the application of mesenchymal stem cells derived from bone marrow. A large number of studies have confirmed that the mesenchymal stem is dry and fine. The cells also exist in a variety of tissues. This experiment uses mesenchymal stem cells derived from umbilical and fat sources to compare with adult mesenchymal stem cells derived from adult bone marrow, hoping to obtain better seed cell sources.
Objective: to isolate and culture mesenchymal stem cells from adult bone marrow, fat and umbilical cord, and compare the morphological characteristics, proliferation ability, surface markers and differentiation ability of three different sources of mesenchymal stem cells, and provide experimental basis and theoretical basis for the selection of cell sources in the clinical application of mesenchymal stem cells.
Methods: in this study, adult bone marrow mesenchymal stem cells were isolated and cultured by direct adherence culture, and mesenchymal stem cells derived from fat and umbilical cord were isolated and cultured by enzyme digestion, and three kinds of mesenchymal stem cells were further purified by adherent culture. The three sources were compared with the microscope technique. The morphological characteristics of the mesenchymal stem cells. The proliferation ability of three mesenchymal stem cells of P3 generation was detected by MTT method. The external transmission capacity was studied by continuous passages in vitro. Flow cytometry was used to detect the surface markers of three P3/P4 generations of mesenchymal stem cells. In vitro, three mesenchymal stem cells were induced to differentiate into adipose and osteoblasts. The differentiation results were identified by oil red 0 and von-Kossa staining.
Results: three kinds of mesenchymal stem cells were cultured in vitro, with a long shuttle type, vortexed and adherent growth. Compared with mesenchymal stem cells derived from umbilical cord, the volume of mesenchymal stem cells from adipose and bone marrow derived mesenchymal stem cells was larger. Through the analysis of growth curve, the multiplication time of three mesenchymal stem cells was found to be the umbilical cord from strong to weak. Derived mesenchymal stem cells derived from mesenchymal stem cells, adipose derived mesenchymal stem cells and bone marrow mesenchymal stem cells. Through long term passages in vitro, we found that mesenchymal stem cells derived from umbilical cord derived mesenchymal stem cells have the strongest passages, but the adipose derived mesenchymal stem cells are the next, and the most poor passages of mesenchymal stem cells are mesenchymal stem cells derived from bone marrow. Three kinds of mesenchymal stem cells are used. The stem cells all express CD90, CD105 (SH2), CD73 (SH3/4), CD166, CD29, and do not express CD31, CD133, CD19, CDllb, CD34, and CDllb (CD34). In addition, three mesenchymal stem cells can differentiate into adipocytes confirmed by oil and red 0 staining.
Conclusion: we successfully isolated and cultured mesenchymal stem cells from adult bone marrow, fat and umbilical cord tissue. There are some differences in the biological characteristics of the three different sources of mesenchymal stem cells, such as the morphology, proliferation and differentiation of mesenchymal stem cells, and the surface markers are basically the same.
Background: telomere is a special structure at the terminal of eukaryotic cells. It is composed of telomere DNA and telomere protein. It plays an important role in maintaining the stability and integrity of the chromosomes. In general, the telomere of the chromosomes of an animal cell is shortened with cell division. When a certain degree is shortened, the cell will stop proliferating and terminating. Granulase is not expressed in general cells and is expressed only in germ cells, stem cells and tumor cells. However, the results of telomerase expression in mesenchymal stem cells are not unified. Adipose derived mesenchymal stem cells are one of the mesenchymal stem cells which are widely used in basic experimental research and clinical application. This study was to determine the stem cells of adipose derived mesenchymal stem cells. The telomerase activity provides a more detailed experimental basis for its future research and clinical application.
Objective: to isolate and identify adult adipose derived mesenchymal stem cells, and to determine whether adult adipose derived mesenchymal stem cells express telomerase activity.
Methods: mesenchymal stem cells were isolated from fat and cultured in accordance with the standard of the International Society for Cellular Therapy, ISCT. In vitro, adult adipose derived mesenchymal stem cells were induced to differentiate into fat and osteoblast cells, identified by oil red 0 and von-Kossa staining. Reverse transcriptase PCR was used. Telomerase repeated sequence amplification, enzyme chain immunoadsorption (TRAP-ELISA), was used to detect the expression of telomerase activity in adipose derived mesenchymal stem cells with different donor sources and different passages.
Results: the study found that the adipose derived mesenchymal stem cells derived from our method were in conformity with the standards proposed by the international cell therapy association. The adipose derived mesenchymal stem cells of different donor sources and different passages did not express telomerase mRNA or telomerase activity.
Conclusion: adult adipose derived mesenchymal stem cells do not express telomerase activity.

【學(xué)位授予單位】：北京協(xié)和醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2009
【分類號(hào)】：R329

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 吳玉卓;陳紅;;臍血間充質(zhì)干細(xì)胞體外誘導(dǎo)成肝細(xì)胞的研究進(jìn)展[J];中國(guó)肝臟病雜志(電子版);2008年02期

2 隆玄;吳曉丹;施勁東;李善群;;間充質(zhì)干細(xì)胞在急性肺損傷中的應(yīng)用進(jìn)展[J];中國(guó)臨床醫(yī)學(xué);2011年03期

3 楊光忠;陳文明;;間充質(zhì)干細(xì)胞的免疫調(diào)節(jié)功能[J];臨床合理用藥雜志;2011年31期

4 王燕茹;張育;;間充質(zhì)干細(xì)胞與類風(fēng)濕關(guān)節(jié)炎[J];實(shí)用醫(yī)學(xué)雜志;2011年16期

5 馮德鵬;李雪莉;范炎;;臍血間充質(zhì)干細(xì)胞的研究進(jìn)展[J];國(guó)際老年醫(yī)學(xué)雜志;2010年05期

6 程慶;白志明;;間充質(zhì)干細(xì)胞歸巢的研究進(jìn)展[J];中國(guó)現(xiàn)代醫(yī)學(xué)雜志;2011年09期

7 傅強(qiáng);;他達(dá)拉非可對(duì)干細(xì)胞產(chǎn)生持久而穩(wěn)定的保護(hù)作用[J];中華男科學(xué)雜志;2011年08期

8 瞿海龍;麻曉靜;張冰;梁璐;;基因修飾的間充質(zhì)干細(xì)胞在急性心肌梗死中的應(yīng)用前景[J];中西醫(yī)結(jié)合心腦血管病雜志;2011年07期

9 郭玲玲;李明;邢爽;羅慶良;;間充質(zhì)干細(xì)胞治療重癥急性放射病研究的新進(jìn)展[J];中國(guó)實(shí)驗(yàn)血液學(xué)雜志;2011年03期

10 禹亞彬;儲(chǔ)建;章世海;卞建民;;間充質(zhì)干細(xì)胞體外分化為胰島β細(xì)胞的影響因素的研究進(jìn)展[J];醫(yī)學(xué)綜述;2011年17期

相關(guān)會(huì)議論文 前10條

1 房佰俊;宋永平;張煈莉;林全德;魏旭東;;脂肪源間充質(zhì)干細(xì)胞治療GVHD分子機(jī)制的初步研究[A];第11次中國(guó)實(shí)驗(yàn)血液學(xué)會(huì)議論文匯編[C];2007年

2 黃金龍;董運(yùn)海;魏翠;徐妍;王影;魯彥瑋;王志軍;郝晨光;;復(fù)合水凝膠負(fù)載人脂肪間充質(zhì)干細(xì)胞在體內(nèi)分化的研究[A];第二屆全國(guó)醫(yī)療美容技術(shù)交流大會(huì)暨高新技術(shù)精品手術(shù)演示會(huì)論文匯編[C];2010年

3 王巧稚;韓藝;趙宏賢;余鴻;劉廣益;;當(dāng)歸誘導(dǎo)人脂肪間充質(zhì)干細(xì)胞向神經(jīng)細(xì)胞分化和毒性檢測(cè)的研究[A];中國(guó)解剖學(xué)會(huì)第十一屆全國(guó)組織學(xué)與胚胎學(xué)青年學(xué)術(shù)研討會(huì)論文匯編[C];2009年

4 王玉紅;陳光輝;;地黃低聚糖對(duì)人脂肪組織源性間充質(zhì)干細(xì)胞分泌肝細(xì)胞生長(zhǎng)因子的影響[A];2010全國(guó)中西醫(yī)結(jié)合危重病、急救醫(yī)學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2010年

5 杜鳳移;王皓;楊樹(shù)龍;趙繪存;楊英;楊軍;;納米纖維支架對(duì)大鼠間充質(zhì)干細(xì)胞向肝細(xì)胞分化的影響[A];天津市生物醫(yī)學(xué)工程學(xué)會(huì)第29屆學(xué)術(shù)年會(huì)暨首屆生物醫(yī)學(xué)工程前沿科學(xué)研討會(huì)論文集[C];2009年

6 郭勇;張西正;郭春;魏嚴(yán);李瑞欣;徐曉瑩;張永紅;;脂肪間充質(zhì)干細(xì)胞向心肌細(xì)胞分化中心肌發(fā)育相關(guān)基因的表達(dá)[A];天津市生物醫(yī)學(xué)工程學(xué)會(huì)2008年年會(huì)暨首屆生物醫(yī)學(xué)工程與臨床論壇論文集[C];2008年

7 朱恒;江小霞;劉元林;張毅;毛寧;;間充質(zhì)干細(xì)胞選擇性調(diào)節(jié)破骨細(xì)胞發(fā)育和功能[A];第13屆全國(guó)實(shí)驗(yàn)血液學(xué)會(huì)議論文摘要[C];2011年

8 張顥;陶艷玲;邱林;張伯龍;馬軍;陳志哲;劉擁軍;韓忠朝;;一種具有免疫負(fù)調(diào)節(jié)功能的人臍帶源間充質(zhì)干細(xì)胞[A];第12屆全國(guó)實(shí)驗(yàn)血液學(xué)會(huì)議論文摘要[C];2009年

9 張勇杰;金巖;胡世頡;陸偉;張華利;姜明;沈楠;劉鵬;;復(fù)合脂肪源性間充質(zhì)干細(xì)胞的重組合神經(jīng)移植物修復(fù)周圍神經(jīng)缺損的實(shí)驗(yàn)研究[A];中華口腔醫(yī)學(xué)會(huì)第七屆全國(guó)口腔病理學(xué)術(shù)會(huì)議論文摘要匯編[C];2006年

10 楊少光;池穎;戎麗娟;邢文;盧士紅;趙欽軍;馬鳳霞;韓忠朝;;不同來(lái)源間充質(zhì)干細(xì)胞誘導(dǎo)分化成血管內(nèi)皮細(xì)胞的比較[A];第13屆全國(guó)實(shí)驗(yàn)血液學(xué)會(huì)議論文摘要[C];2011年

相關(guān)重要報(bào)紙文章 前10條

1 王秋月　王琳;空軍總醫(yī)院采用間充質(zhì)干細(xì)胞救治小腦萎縮[N];科技日?qǐng)?bào);2009年

2 記者 李素鋒;我市首例間充質(zhì)干細(xì)胞移植手術(shù)取得成功[N];臨汾日?qǐng)?bào);2009年

3 記者 王丹　通訊員 艾素;異染性腦白質(zhì)營(yíng)養(yǎng)不良治療獲突破[N];健康報(bào);2010年

4 記者 白毅;間充質(zhì)干細(xì)胞可促進(jìn)成熟樹(shù)突狀細(xì)胞增殖分化[N];中國(guó)醫(yī)藥報(bào);2009年

5 張泓;生物醫(yī)藥,2008新突破[N];北方經(jīng)濟(jì)時(shí)報(bào);2008年

6 徐機(jī)玲;姜躍進(jìn);我國(guó)骨髓干細(xì)胞移植技術(shù)獲突破[N];中國(guó)醫(yī)藥報(bào);2003年

7 本報(bào)記者 楊陽(yáng)騰;北科生物：讓干細(xì)胞創(chuàng)造醫(yī)學(xué)奇跡[N];經(jīng)濟(jì)日?qǐng)?bào);2011年

8 時(shí)報(bào)記者 楊曉帆;韓忠朝:中國(guó)干細(xì)胞研究領(lǐng)軍者[N];濱海時(shí)報(bào);2010年

9 張獻(xiàn)懷 王志紅;抗排異反應(yīng)有了新辦法[N];保健時(shí)報(bào);2009年

10 徐機(jī)玲 姜躍進(jìn);骨髓干細(xì)胞移植我國(guó)實(shí)現(xiàn)新突破[N];新華每日電訊;2003年

相關(guān)博士學(xué)位論文 前10條

1 吳桂珠;脂肪間充質(zhì)干細(xì)胞治療壓力性尿失禁的實(shí)驗(yàn)研究[D];福建醫(yī)科大學(xué);2010年

2 彭飛;620nm非相干紅光對(duì)大鼠骨髓間充質(zhì)干細(xì)胞的光生物調(diào)節(jié)作用[D];華中科技大學(xué);2011年

3 于美嬌;系統(tǒng)歸巢的間充質(zhì)干細(xì)胞在牙周組織修復(fù)再生過(guò)程中的作用研究[D];山東大學(xué);2011年

4 李東杰;人臍帶間充質(zhì)干細(xì)胞促進(jìn)創(chuàng)面愈合及體外誘導(dǎo)分化為表皮樣細(xì)胞的實(shí)驗(yàn)研究[D];中國(guó)人民解放軍軍醫(yī)進(jìn)修學(xué)院;2011年

5 李寶軍;脂肪間充質(zhì)干細(xì)胞體外誘導(dǎo)及復(fù)合PLGA體內(nèi)異位成軟骨的實(shí)驗(yàn)研究[D];中南大學(xué);2007年

6 朱雅姝;Flk-1~+間充質(zhì)干細(xì)胞對(duì)腫瘤細(xì)胞增殖的抑制作用及其分子機(jī)制研究[D];中國(guó)協(xié)和醫(yī)科大學(xué);2008年

7 熊卉;轉(zhuǎn)化生長(zhǎng)因子β1基因體外轉(zhuǎn)染兔顳下頜關(guān)節(jié)滑膜間充質(zhì)干細(xì)胞向纖維軟骨轉(zhuǎn)化實(shí)驗(yàn)研究[D];武漢大學(xué);2010年

8 苗宗寧;胎盤間充質(zhì)干細(xì)胞與絲素蛋白/羥基磷灰石材料在骨創(chuàng)傷修復(fù)中的實(shí)驗(yàn)研究[D];蘇州大學(xué);2010年

9 梁偉;人骨髓Flk-1~+間充質(zhì)干細(xì)胞抗DNA損傷物質(zhì)影響作用研究[D];中國(guó)協(xié)和醫(yī)科大學(xué);2008年

10 趙迎澤;BMP9通過(guò)MAPKs通路調(diào)控間充質(zhì)干細(xì)胞成骨分化及其機(jī)制的初步研究[D];重慶醫(yī)科大學(xué);2010年

相關(guān)碩士學(xué)位論文 前10條

1 章守琴;人羊膜間充質(zhì)干細(xì)胞支持造血的體外研究[D];昆明醫(yī)學(xué)院;2010年

2 陳芳;臍帶間充質(zhì)干細(xì)胞修復(fù)化療所致卵巢顆粒細(xì)胞損傷的體外實(shí)驗(yàn)[D];暨南大學(xué);2010年

3 張茜真;人臍帶間充質(zhì)干細(xì)胞的分離、鑒定以及干細(xì)胞特異性轉(zhuǎn)錄因子誘導(dǎo)其重編程的研究[D];浙江理工大學(xué);2010年

4 汲晶;脂肪與骨髓來(lái)源的間充質(zhì)干細(xì)胞誘導(dǎo)分化為神經(jīng)細(xì)胞的比較[D];山西醫(yī)科大學(xué);2010年

5 陳義;臍帶間充質(zhì)干細(xì)胞培養(yǎng)及體外重建角膜后板層的初步研究[D];暨南大學(xué);2010年

6 唐子濱;納米級(jí)膠原基骨材料復(fù)合自體脂肪間充質(zhì)干細(xì)胞用于兔后外側(cè)脊柱融合的實(shí)驗(yàn)研究[D];河北醫(yī)科大學(xué);2010年

7 齊凱;人臍帶來(lái)源間充質(zhì)干細(xì)胞分離培養(yǎng)方法的優(yōu)化初探[D];山西醫(yī)科大學(xué);2011年

8 馬蘭蘭;不同胎齡人臍帶血間充質(zhì)干細(xì)胞的研究[D];中國(guó)醫(yī)科大學(xué);2010年

9 李成華;口腔黏膜間充質(zhì)干細(xì)胞存在及在口腔扁平苔蘚中變化的初步研究[D];第四軍醫(yī)大學(xué);2010年

10 田毅;人臍帶Wharton's jelly間充質(zhì)干細(xì)胞的生物學(xué)特性以及其與腦腫瘤干細(xì)胞共培養(yǎng)的實(shí)驗(yàn)研究[D];鄭州大學(xué);2010年

，

本文編號(hào)：1852835