本文選題：超順磁性氧化鐵 + 骨髓間充質(zhì)干細胞��； 參考：《山西醫(yī)科大學》2010年碩士論文

【摘要】： 背景：一些研究者利用超順磁性氧化鐵對骨髓間充質(zhì)干細胞進行標記,并利用MRI技術(shù)對標記細胞肝內(nèi)、腎內(nèi)移植后進行初步的活體示蹤,但是,對于超順磁性氧化鐵標記骨髓間充質(zhì)干細胞后,對其存活、增殖以及分化是否有影響研究較少。 目的：觀察超順磁性氧化鐵標記骨髓間充質(zhì)干細胞后,對其存活、增殖以及向神經(jīng)細胞的分化是否有影響。 方法：使用超順磁性氧化鐵標記大鼠骨髓間充質(zhì)干細胞,采用普魯士藍染色法鑒定其標記率、臺盤藍染色法檢測細胞活力、MTT法檢測標記干細胞的細胞增值活力、以1 mmol／Lβ-巰基乙醇及無血清DMEM培養(yǎng)液體外誘導標記細胞向神經(jīng)細胞分化并用免疫組織化學鑒定誘導后細胞、再次使用普魯士藍染色法鑒定神經(jīng)細胞內(nèi)的鐵顆粒。 結(jié)果與結(jié)論：普魯士藍染色對干細胞的標記率接近100%,臺盤藍染色顯示標記細胞的存活率不受影響；MTT法檢測發(fā)現(xiàn)標記干細胞的增殖活力與未標記干細胞相比差異無顯著性意義(P0.05)；以β-巰基乙醇誘導后大部分骨髓間充質(zhì)干細胞分化為神經(jīng)元樣細胞、免疫組織化學陽性,再次普魯士藍染色顯示鐵顆粒位于神經(jīng)細胞的細胞漿內(nèi)。提示超順磁性氧化鐵標記骨髓間充質(zhì)干細胞后,對于干細胞的存活、增殖以及向神經(jīng)細胞的分化無影響。
[Abstract]:Background: some researchers used superparamagnetic ferric oxide to label bone marrow mesenchymal stem cells and MRI technique was used to trace the labeled cells in liver and kidney, but, There are few studies on the survival, proliferation and differentiation of bone marrow mesenchymal stem cells labeled with superparamagnetic iron oxide. Aim: to investigate the effects of superparamagnetic ferric oxide on the survival, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs). Methods: superparamagnetic ferric oxide was used to label rat bone marrow mesenchymal stem cells (BMSCs). Prussian blue staining was used to identify the labeling rate, and table-blue staining was used to detect cell viability. MTT assay was used to detect the cell proliferation activity of labeled stem cells. The labeled cells were induced to differentiate into neural cells with 1 mmol/L 尾 -mercaptoethanol and serum-free DMEM in vitro. The induced cells were identified by immunohistochemistry, and the iron granules in the neurons were identified by Prussian blue staining. Results and conclusion: the labeling rate of stem cells by Prussian blue staining was close to 100. The viability of labeled stem cells was not affected by plate blue staining. The proliferative activity of labeled stem cells was not different from that of unlabeled stem cells by MTT assay. Most bone marrow mesenchymal stem cells differentiated into neuron-like cells after induced by 尾 -mercaptoethanol. Immunohistochemical staining showed that iron granules were located in the cytoplasm of nerve cells. These results suggest that superparamagnetic ferric oxide labeled bone marrow mesenchymal stem cells has no effect on the survival, proliferation and differentiation of stem cells.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R329

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本文編號：1832549