發(fā)布時間：2018-04-12 19:33

  本文選題：野百合堿 + 肺動脈高壓��； 參考：《青島大學(xué)》2013年碩士論文

【摘要】：摘要目的：采用野百合堿(monocrotaline, MCT)誘導(dǎo)建立SD大鼠肺動脈高壓模型,探討野百合堿誘導(dǎo)肺動脈高壓模型的穩(wěn)定性及量效與時效關(guān)系,尋求野百合堿誘導(dǎo)肺動脈高壓模型的最佳時間和劑量。方法：清潔級SD雄性大鼠110只,隨機(jī)分為C組、M1、M2、M3和M4組,每組各15只,H1、H2和H3組,每組各12只。其中C組為對照組,給予相同劑量溶劑腹腔注射。M1～M4組大鼠分別一次性腹腔注射MCT30、40、50和60mg/kg,4周后比較各組大鼠的生存率、檢測平均肺動脈壓力(mPAP),肺動脈收縮壓(PASP),右心室收縮壓(RVSP),右心肥大指數(shù)(RVH1),并取肺組織行蘇木素-伊紅染色,觀察肺的病理改變,進(jìn)行肺小動脈形態(tài)計量學(xué)指標(biāo)檢測并通過肺微血管密度(MVD)的測定來綜合判斷肺動脈高壓模型的穩(wěn)定性。而H1～H3組分別一次性腹腔注射MCT50mg/kg,分別于2周、4周、6周后檢測以上指標(biāo)。結(jié)果：C組無死亡,M1～M4組大鼠的生存率分別為87%,87%,67%,40%。M1～M4各組SD大鼠平均肺動脈壓、肺動脈收縮壓、右心肥大指數(shù)依次增大(p＜0.05),肺小動脈形態(tài)計量學(xué)指標(biāo)檢測顯示肺血管中膜厚度百分比依次增大(p＜0.05),肺微血管密度依次減小(p＜0.05),而M3與M4組測量結(jié)果無明顯差異。H2和H3組各項(xiàng)指標(biāo)較H1組有明顯差異,而H2和H3兩組之間各項(xiàng)指標(biāo)無明顯差異。結(jié)論：腹腔注射50mg/kg劑量野百合堿4周后可誘導(dǎo)穩(wěn)定肺動脈高壓模型,是野百合堿誘導(dǎo)肺動脈高壓的最佳劑量與時間。
[Abstract]:Objective: to establish a model of pulmonary hypertension in SD rats induced by monocrotaline (MCTT), and to investigate the stability, dose-effect and time-effect of monocrotaline induced pulmonary hypertension in SD rats.To find the best time and dose of monocrotaline induced pulmonary hypertension model.Methods: 110 male SD rats of clean grade were randomly divided into two groups: group C (n = 15) and group M _ 4 (n = 15) with H1H _ 2 and H _ 3, with 12 rats in each group.Group C was used as control group. The survival rate of rats in group C was compared after 4 weeks of intraperitoneal injection of MCT30 + 4050 and 60mg / kg respectively.The mean pulmonary artery pressure (MPP), pulmonary systolic pressure (PAP), right ventricular systolic pressure (RV), right cardiac fat index (RVH1) were measured. The lung tissues were stained with hematoxylin and eosin to observe the pathological changes of the lung.The stability of pulmonary hypertension model was evaluated by the measurement of pulmonary arterioles morphometry and the determination of pulmonary microvessel density (MVD).In H1~H3 group, 50 mg / kg MCT was injected intraperitoneally, and the above indexes were detected after 2 weeks and 4 weeks and 6 weeks.Results the survival rate of M1M4 rats without death was 87 and 87, respectively. The mean pulmonary artery pressure and pulmonary systolic pressure of SD rats in M1M4 group were significantly higher than those in control group 40. M1 and M4 groups, and the mean pulmonary arterial pressure and pulmonary systolic pressure in each group were significantly higher than those in control group (P < 0. 05).The right heart fat index increased in turn (P < 0.05), the pulmonary arterioles morphometrics showed that the percentage of pulmonary vascular media thickness increased in turn, and the pulmonary microvessel density decreased in turn, but there was no significant difference between M3 group and M4 group.There were significant differences between H1 group and H1 group.There was no significant difference between H _ 2 and H _ 3 groups.Conclusion: the stable pulmonary hypertension model can be induced by intraperitoneal injection of monocrotaline for 4 weeks, which is the best dose and time for monocrotaline to induce pulmonary hypertension.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R544.1;R-332

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 黃維佳;覃家錦;;肺動脈高壓的動物模型研究[J];廣西醫(yī)科大學(xué)學(xué)報;2011年06期

2 劉斌;王獻(xiàn)民;魏麗;周同甫;劉瀚e，

本文編號：1741115