本文選題：小腸結(jié)腸炎耶爾森菌　切入點：分子流行病學(xué)　出處：《中國疾病預(yù)防控制中心》2009年碩士論文

【摘要】： 小腸結(jié)腸炎耶爾森菌是一種重要的全球分布的人獸共患腸道致病菌,在人、多種動物、食品及外環(huán)境中都有分布。為闡明中國小腸結(jié)腸炎耶爾森菌分布特征,本研究對我國1980～2008年從腹瀉病人、宿主動物、食品及環(huán)境分離到的1295株小腸結(jié)腸炎耶爾森菌,進行流行病學(xué)分布特征與血清型、毒力基因、脈沖場凝膠電泳(PFGE)分型等病原學(xué)特征的分析。結(jié)果表明,中國小腸結(jié)腸炎耶爾森菌地域分布廣泛、宿主多樣、血清型眾多。而致病性小腸結(jié)腸炎耶爾森菌目前則僅存在于0:3和0:9兩個血清型,且多分布于北方寒冷地區(qū),但在少數(shù)溫暖的沿海地區(qū),0:3血清型致病性菌株也有分布。在同一地區(qū)內(nèi),從腹瀉病人分離到的致病性菌株與部分動物源性菌株的PFGE型別一致,提示家畜家禽是人類感染小腸結(jié)腸炎耶爾森菌的重要來源,而豬是造成我國人類感染的最主要宿主,同時也證實農(nóng)家犬也是引起我國部分地區(qū)人群感染的傳染源。 本研究還第一次對小腸結(jié)腸炎耶爾森菌進行了多位點序列分析(MLST),闡釋小腸結(jié)腸炎耶爾森菌MLST型別特征,并探索小腸結(jié)腸炎耶爾森菌與假結(jié)核耶爾森菌、鼠疫耶爾森菌的親緣關(guān)系。結(jié)果表明,小腸結(jié)腸炎耶爾森菌與假結(jié)核耶爾森菌在MLST分型中分別屬于兩個不同類,鼠疫耶爾森菌則屬于假結(jié)核耶爾森菌的一個分支。小腸結(jié)腸炎耶爾森菌MLST分析明顯的形成致病性菌株與非致病性菌株兩大類別。致病性菌株ST型別集中,圍繞血清型呈現(xiàn)出高度的克隆性;而非致病性菌株型別分散,沒有明顯的流行病學(xué)關(guān)聯(lián),呈現(xiàn)明顯的多態(tài)性。
[Abstract]:Yersinia enterocolitica (Yersinia enterocolitica) is an important global distribution of zoonotic enteropathogenic bacteria, distributed in human, animal, food and external environment, in order to elucidate the distribution characteristics of Yersinia enterocolitica in China, in order to elucidate the distribution of Yersinia enterocolitica in China. In this study, 1295 strains of Yersinia enterocolitica isolated from diarrhea patients, host animals, food and environment in China from 1980 to 2008 were studied. The epidemiological distribution, serotype and virulence genes of Yersinia enterocolitica were studied. The results showed that Yersinia enterocolitica was widely distributed in China, and its host was diverse. However, Yersinia enterocolitica is only present at 0:3 and 0:9, and most of the serotypes are distributed in cold areas of northern China. But in a few warm coastal areas, serotypic pathogenic strains were also distributed. In the same region, the PFGE types of pathogenic strains isolated from diarrhea patients were the same as those of some animal-derived strains. It is suggested that livestock and poultry are important sources of Yersinia enterocolitica infection in humans, and pigs are the main hosts of human infection in China, and it is also confirmed that farm dogs are also the source of infection in some parts of China. The multilocus sequence analysis of Yersinia enterocolitica was carried out for the first time to elucidate the characteristics of Yersinia enterocolitica MLST and to explore Yersinia enterocolitica and Yersinia pseudotuberculosis. The results showed that Yersinia enterocolitica and Yersinia pseudotuberculosis belong to two different classes in MLST typing. Yersinia pestis belongs to a branch of Yersinia pseudotuberculosis. The MLST analysis of Yersinia enterocolitica shows that there are two main types of pathogenic and non-pathogenic strains. The serotypes were highly clonal and non-pathogenic strains were dispersed and had no obvious epidemiological association and showed obvious polymorphism.
【學(xué)位授予單位】：中國疾病預(yù)防控制中心
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2009
【分類號】：R378

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本文編號：1697878