本文選題：動(dòng)物模型　切入點(diǎn)：脊柱結(jié)核　出處：《新疆醫(yī)科大學(xué)》2010年碩士論文

【摘要】：目的:在新西蘭白兔腰椎局部鉆孔種植人型結(jié)核桿菌H37Rv標(biāo)準(zhǔn)菌株構(gòu)建脊柱結(jié)核疾病模型,為以后骨結(jié)核的基礎(chǔ)研究提供動(dòng)物模型。方法:對(duì)未致敏的新西蘭白兔的腰5椎體上方近椎間盤處鉆骨孔,填充明膠海綿,吸附結(jié)核菌懸液(菌量:0.5mg/0.1ml)建立模型,同時(shí)設(shè)置生理鹽水對(duì)照組及空白組,術(shù)后行大體觀察、影像學(xué)、組織病理學(xué)、細(xì)菌學(xué)等檢查證實(shí)。結(jié)果:新西蘭白兔腰椎在行H37Rv結(jié)核菌株感染后,局部反應(yīng)較明顯,全身反應(yīng)較輕。感染組9只兔完成實(shí)驗(yàn)(9/12),其中3只出現(xiàn)消瘦、納差等癥狀(3/9);術(shù)后1月X線陽性兔2只(2/9),術(shù)后2月X線陽性兔4只(4/9),術(shù)后2月CT陽性兔6只(6/9),術(shù)后2月MRI陽性兔7只(7/9);感染組陽性兔的椎體、椎旁軟組織切片HE染色示有膿細(xì)胞、類上皮樣細(xì)胞或壞死灶等形成,感染組中膿腫形成的兔膿液培養(yǎng)示60.0%結(jié)核分枝桿菌生長。建立模型成功率為66.7%(6/9)。結(jié)論:通過在未致敏的兔脊柱椎體局部進(jìn)行適當(dāng)劑量的H37Rv結(jié)核標(biāo)準(zhǔn)菌株的種植感染,可以構(gòu)建出與人類脊柱結(jié)核病理變化相似的兔脊柱結(jié)核。通過癥狀、影像、組織病理、細(xì)菌學(xué)等手段,可以成功地證實(shí)模型。
[Abstract]:Objective: to establish a model of spinal tuberculosis disease in New Zealand white rabbit lumbar vertebrae implanted with standard strain of Mycobacterium tuberculosis (H37Rv). Methods: an animal model was established on the unsensitized New Zealand white rabbits with bone holes near the intervertebral disc above the lumbar 5 vertebrae, filling with gelfoam, and adsorbing the suspension of tuberculous bacteria (0. 5 mg / 0.1 ml). At the same time, normal saline control group and blank group were set up, the gross observation, imaging, histopathology and bacteriology were performed after operation. Results: the local reaction of the lumbar vertebrae of New Zealand white rabbits was obvious after the infection of H37Rv tuberculous bacilli. The whole body reaction was mild. Nine rabbits in the infected group completed the experiment 9 / 12, and 3 of them were emaciated. Anorexia and other symptoms were 3 / 9 / 9; X-ray positive rabbits were 2 / 9 / 9 in 1 month after operation, 4 / 9 / 9 in X ray positive rabbits in 2 months after operation, 6 / 9 / 9 in CT positive rabbits in 2 months after operation, 7 / 9 / 9 in MRI positive rabbits in 2 months after operation; vertebrae of positive rabbits in infection group. He staining of paravertebral soft tissue sections showed the formation of pyogenic cells, epithelioid cells or necrotic foci. In the infection group, the rabbit abscess culture showed that the mycobacterium tuberculosis grew in 60.0%. The success rate of the model was 66.70.Conclusion: the appropriate dose of H37Rv TB standard strain was implanted in the unsensitized spinal vertebrae of rabbits. The rabbit spinal tuberculosis which is similar to the pathological changes of human spinal tuberculosis can be successfully confirmed by means of symptoms, images, histopathology, bacteriology and so on.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R-332;R529.2

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 劉瑩;MR-DWI診斷早期脊柱結(jié)核的動(dòng)物實(shí)驗(yàn)研究[D];新疆醫(yī)科大學(xué);2012年

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本文編號(hào)：1684327