異種關節(jié)軟骨與MSCs體外共同培養(yǎng)的實驗研究
發(fā)布時間:2018-03-07 19:01
本文選題:間充質(zhì)干細胞 切入點:關節(jié)軟骨細胞 出處:《實用臨床醫(yī)藥雜志》2010年11期 論文類型:期刊論文
【摘要】:目的提供一種新的種子細胞獲得方法,解決軟骨缺損修復過程中軟骨數(shù)量不足的困難。方法人骨髓間充質(zhì)干細胞體外擴增誘導分化后與兔的關節(jié)軟骨細胞按不同比例共同培養(yǎng)利用real-time PCR技術(shù)分析細胞表型的表達情況。結(jié)果實驗組2種細胞共培養(yǎng)4周后,混合培養(yǎng)顯示正反饋調(diào)節(jié)軟骨細胞的增殖,軟骨細胞外基質(zhì)、SOX9、Ⅱ型膠原基因表達增加,軟骨細胞生成明顯增加。結(jié)論間充質(zhì)干細胞以旁分泌或自分泌的方式調(diào)節(jié)間充質(zhì)干細胞向軟骨細胞的轉(zhuǎn)化。
[Abstract]:Objective to provide a new method for obtaining seed cells. Methods Human bone marrow mesenchymal stem cells were cultured with rabbit articular chondrocytes in different proportions to analyze the cell surface by real-time PCR. Results the two kinds of cells were co-cultured for 4 weeks in the experimental group. Mixed culture showed that positive feedback regulated the proliferation of chondrocytes, and the expression of extracellular matrix SOX9 and type 鈪,
本文編號:1580549
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