本文關(guān)鍵詞： 瘢痕 成纖維細(xì)胞 細(xì)胞外基質(zhì) 多功能蛋白聚糖 轉(zhuǎn)化生長(zhǎng)因子β1　出處：《昆明醫(yī)學(xué)院》2010年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】： [目的]通過(guò)測(cè)定多功能蛋白聚糖(versican)在瘢痕組織及瘢痕成纖維細(xì)胞中的表達(dá)情況,探討其在增生性瘢痕和瘢痕疙瘩形成中的作用,以及versican和TGF-β1的表達(dá)關(guān)系,為臨床預(yù)防和治療瘢痕提供病理學(xué)及基因水平的理論依據(jù)。 [方法](1)對(duì)正常皮膚及病理性瘢痕成纖維細(xì)胞進(jìn)行體外培養(yǎng),采用光鏡觀察、電鏡檢查對(duì)成纖維細(xì)胞形態(tài)、活性、凋亡,RNA提取分析,用實(shí)時(shí)熒光定量PCR檢測(cè)veriscan以及其四種亞型和TGF-β1的mRNA的表達(dá)情況；(2)采用組織塊石蠟切片對(duì)病理性瘢痕組織中的versican和TGF-β1進(jìn)行免疫組化檢測(cè),并運(yùn)用SPSS 17.0軟件對(duì)使用數(shù)據(jù)進(jìn)行t檢驗(yàn),通過(guò)以上實(shí)驗(yàn)觀察分析,研究versican對(duì)增生性瘢痕、瘢痕疙瘩以及成纖維細(xì)胞生物學(xué)行為的影響及病理改變；(3)對(duì)照實(shí)時(shí)熒光定量PCR和免疫組化結(jié)果,分析versican和TGF-β1的表達(dá)關(guān)系。 [結(jié)果](1)光鏡下與正常皮膚成纖維細(xì)胞相比,病理性瘢痕成纖維細(xì)胞排列極性消失,細(xì)胞體積較大,形態(tài)不規(guī)則；(2)掃描電鏡下：病理性瘢痕成纖維細(xì)胞粗面內(nèi)質(zhì)網(wǎng)大量增多,并擴(kuò)展成囊,胞質(zhì)內(nèi)微絲、微管增多,表明其合成蛋白及膠原纖維的功能活躍;(3)免疫組化提示病理性瘢痕組織切片中versican主要表達(dá)于膠原纖維及成纖維細(xì)胞中,而真皮組織中未見(jiàn)明顯表達(dá),病理性瘢痕組織中versican和TGF-β1表達(dá)量較正常皮膚明顯升高,具有統(tǒng)計(jì)學(xué)意義(P0.05)；(4)實(shí)時(shí)熒光定量PCR檢測(cè)結(jié)果示瘢痕疙瘩以及正常瘢痕成纖維細(xì)胞中versican及其四種亞型均有明顯表達(dá),瘢痕疙瘩versican和TGF-β1的mRNA表達(dá)均較正常瘢痕及正常皮膚成纖維細(xì)胞升高。 [結(jié)論]Versican及其四種亞型在瘢痕疙瘩及正常瘢痕成纖維細(xì)胞中有明顯表達(dá),可能對(duì)病理性瘢痕發(fā)生發(fā)展及其成纖維細(xì)胞的生長(zhǎng)、增殖及膠原合成起促進(jìn)作用；versican的表達(dá)與TGF-β1相關(guān),共同促進(jìn)病理性瘢痕的發(fā)生與發(fā)展。
[Abstract]:[objective] to investigate the expression of versican and TGF- 尾 1 in hypertrophic scar and keloid by detecting the expression of multifunctional proteoglycan in scar tissue and scar fibroblasts. To provide the theoretical basis of pathology and gene level for clinical prevention and treatment of scar. [methods] normal skin and pathological scar fibroblasts were cultured in vitro. The morphology, activity and apoptosis of fibroblasts were detected by light microscope and electron microscope. The expression of veriscan, its four subtypes and the mRNA expression of TGF- 尾 1 were detected by real-time fluorescence quantitative PCR. The tissue paraffin sections were used to detect versican and TGF- 尾 1 in pathological scar tissue by immunohistochemistry. T test was performed with SPSS 17.0 software. The effect of versican on the biological behavior of hypertrophic scar, keloid and fibroblasts and its pathological changes were studied. The expression of versican and TGF- 尾 1 were analyzed by real-time quantitative PCR and immunohistochemistry. [results] compared with normal skin fibroblasts under light microscope, pathological scar fibroblasts disappeared in polarity, larger in size and irregular in shape. Under scanning electron microscope, the rough endoplasmic reticulum of pathological scar fibroblasts increased significantly. The expression of versican in the sections of pathological scar tissues was mainly expressed in collagen fibers and fibroblasts, which indicated that the synthesis protein and the function of collagen fibers were active. However, the expression of versican and TGF- 尾 1 in pathological scar tissue was significantly higher than that in normal skin. The results of real-time fluorescence quantitative PCR analysis showed that versican and its four subtypes were significantly expressed in keloid and normal scar fibroblasts. The mRNA expression of versican and TGF- 尾 1 in keloid was higher than that in normal scar and normal skin fibroblasts. [conclusion] the expression of Versican and its four subtypes in keloid and normal scar fibroblasts may play an important role in the development of pathological scar and the growth of fibroblasts. The expression of TGF- 尾 1 may play a role in promoting proliferation and collagen synthesis in keloid and normal scar fibroblasts. To promote the occurrence and development of pathological scar.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類(lèi)號(hào)】：R363

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