本文關鍵詞：CD200蛋白質(zhì)分子三維結(jié)構(gòu)的構(gòu)建　出處：《吉林大學》2008年碩士論文　論文類型：學位論文

  更多相關文章： CD200 免疫超家族 蛋白質(zhì)三維結(jié)構(gòu) 同源模建

【摘要】： CD200分子是自1979年由Mc Master發(fā)現(xiàn)的。它屬于免疫球蛋白超家族,是一種I-型膜糖蛋白,胞膜外區(qū)有兩個免疫球蛋白樣結(jié)構(gòu)域。CD200在人類和嚙齒類動物均有表達,廣泛分布在中樞和外周神經(jīng)系統(tǒng)、胸腺細胞、淋巴細胞、血管內(nèi)皮細胞、平滑肌細胞以及卵巢細胞和神經(jīng)元。具有介導T細胞共刺激信號,促進T細胞增殖,使1型細胞因子減少,2型細胞因子增加的作用,從而發(fā)揮誘導同種和異種的移植耐受,延長移植物存活時間的功能。CD200胞內(nèi)僅有19個氮基酸,缺乏任何信號序列,也沒有作為信號銜接蛋白相互作用所需的跨膜錨定位點,因此其功能效應需要表達于其他細胞表面相應的受體參與。CD200與CD200Rs結(jié)合后,通過胞內(nèi)NPXY序列或跨膜區(qū)與信號銜接分子結(jié)合產(chǎn)生效應,但其分子機制不明。 蛋白質(zhì)的功能主要取決于它們的三維結(jié)構(gòu)、運動及相互作用。對蛋白質(zhì)結(jié)構(gòu)的解析可以從根本上闡明蛋白質(zhì)功能的分子機制和基礎,同時也是研究蛋白質(zhì)功能的一個重途徑。本研究利用同源模建的方法對CD200分子進行了蛋白質(zhì)三維結(jié)構(gòu)預測的理論研究。 主要結(jié)果:通過同源模建和分子動力學模擬,得到了CD200的三維結(jié)構(gòu)。研究表明CD200分子在化學結(jié)構(gòu)上是對稱的。為CD200在前期的實驗提供了理論依據(jù)。 結(jié)論:通過從蛋白質(zhì)三維結(jié)構(gòu)預測的理論研究中,獲得了CD200分子的一個對稱的分子結(jié)構(gòu)蛋白質(zhì)三維構(gòu)象。從理論基礎上為CD200分子的功能及作用提供了有力依據(jù)。
[Abstract]:The CD200 molecule was discovered by MC Master in 1979. It belongs to the immunoglobulin superfamily and is an I- type membrane glycoprotein. There are two immunoglobulin-like domains. CD200 is expressed in human and rodent. It is widely distributed in the central and peripheral nervous system, thymocytes, lymphocytes, vascular endothelial cells. Smooth muscle cells, ovarian cells and neurons can mediate T cell costimulatory signal, promote T cell proliferation, and make type 1 cytokines decrease the increase of type 2 cytokines. Thus, the function of inducing allogeneic and xenogeneic transplantation tolerance, prolonging the survival time of the graft. CD200 has only 19 azo acids in the cell, and lacks any signal sequence. There is no transmembrane anchoring site required for the interaction of signal junction proteins, so its functional effects need to be expressed in the corresponding receptors on the surface of other cells involved in the binding of. CD200 to CD200Rs. The molecular mechanism of NPXY sequence or transmembrane region binding to signal junction molecules is unclear. The function of protein mainly depends on their three-dimensional structure, movement and interaction. The analysis of protein structure can explain the molecular mechanism and basis of protein function. At the same time, it is also a important way to study the function of protein. In this study, we used the homology modeling method to predict the three-dimensional structure of CD200 molecule. Main results: the molecular dynamics simulation of homologous modeling was carried out. The three-dimensional structure of CD200 is obtained. The results show that the chemical structure of CD200 is symmetrical, which provides a theoretical basis for the previous experiments of CD200. Conclusion: from the theoretical study of protein three-dimensional structure prediction. The three-dimensional conformation of a symmetric molecular structure protein of CD200 molecule has been obtained, which provides a powerful basis for the function and action of CD200 molecule on the basis of theory.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2008
【分類號】：R392.11

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