本文關(guān)鍵詞：流體剪切力對(duì)小鼠胚胎成骨細(xì)胞增殖及細(xì)胞周期的影響　出處：《蘭州大學(xué)》2010年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 小鼠胚胎成骨細(xì)胞系mc3t3-el細(xì)胞 流體剪切力 細(xì)胞增殖 細(xì)胞周期

【摘要】： 目的：探討流體剪切力對(duì)小鼠胚胎成骨細(xì)胞系mc3t3-el細(xì)胞增殖及細(xì)胞周期的影響。 方法：小鼠胚胎成骨細(xì)胞系mc3t3-el細(xì)胞受強(qiáng)度為12 dyne/cm2的流體剪切力刺激60分鐘,之后應(yīng)用四甲基偶氮唑藍(lán)(MTT)法和流式細(xì)胞儀來(lái)檢測(cè)細(xì)胞的增殖能力和細(xì)胞周期。 結(jié)果：經(jīng)過(guò)流體剪切力作用后,小鼠胚胎成骨細(xì)胞系mc3t3-el細(xì)胞增殖能力明顯提高,細(xì)胞活性明顯增強(qiáng)。加力組S期細(xì)胞的百分比為(14.67-1.18)%,較對(duì)照組S期細(xì)胞的百分比(8.05-1.08)%,約增高82.2%(p0.01)。 結(jié)論：流體剪切力具有提高小鼠胚胎成骨細(xì)胞系mc3t3-el細(xì)胞增殖能力以及增強(qiáng)其細(xì)胞活性的作用。
[Abstract]:Objective: To investigate the effect of fluid shear force on the proliferation and cell cycle of mouse embryonic osteoblast line mc3t3-el cells.
Methods: mouse embryonic osteoblast cell line mc3t3-el was stimulated by shear force of 12 dyne/cm2 for 60 minutes. Then, the proliferation and cell cycle of cells were detected by four methazolam blue (MTT) and flow cytometry.
Results: after fluid shear force, the proliferation ability of mc3t3-el cells increased significantly, and the activity of cells increased significantly. The percentage of S phase cells in the afterburner group was (14.67-1.18)%, which was increased by 82.2% (P0.01) compared with the percentage of S phase cells (8.05-1.08)% in the control group.
Conclusion: fluid shear force can enhance the proliferation of mouse embryonic osteoblast mc3t3-el cell line and enhance its cell activity.

【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R329

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