本文關(guān)鍵詞：體外擴增巨核細胞的研究　出處：《中國協(xié)和醫(yī)科大學(xué)》2009年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 臍血單個核細胞 CD34~+細胞 胎肺間充質(zhì)干細胞 巨核細胞

【摘要】： 目的:探討采用臍血單個核細胞為起始細胞在無血清培養(yǎng)基中體外擴增巨核細胞;觀察胎兒肺部組織來源的間充質(zhì)干細胞對臍血單個核細胞誘導(dǎo)分化為巨核細胞的影響。方法:分取臍血單個核細胞,在TPO,IL—11,肝素的擴增體系中分為兩組培養(yǎng),第一組以CD34~+細胞作為對照,單獨培養(yǎng),第二組與間充質(zhì)干細胞共培養(yǎng),在7天,10天,14天時,取細胞進行活細胞計數(shù),流式細胞儀分析CD41a和CD61的表達情況。取14天細胞采用免疫組化和透射電鏡分析細胞形態(tài)與結(jié)構(gòu),通過流式細胞儀分析細胞DNA含量。結(jié)果:MNC經(jīng)細胞因子誘導(dǎo)后,CD41a和CD61陽性比例明顯上升,與0天時相比,培養(yǎng)14天后,CD41a~+細胞平均擴增了429.5倍,CD61~+細胞平均擴增了339倍。第二組在10天時得到的CD41~+細胞和CD61~+細胞最多,分別是第一組的4.5倍和4.7倍,免疫組化和電鏡結(jié)果表明,兩組細胞核發(fā)育不成熟。結(jié)論:在TPO,IL—11,肝素的擴增體系下以MNC為起始細胞能夠誘導(dǎo)得到大量的巨核細胞,胎肺間充質(zhì)干細胞能有效刺激CD41~+細胞和CD61~+細胞生成,對細胞核發(fā)育影響。
[Abstract]:Objective: to investigate the expansion of megakaryocytes from cord blood mononuclear cells in serum-free medium in vitro. To observe the effect of mesenchymal stem cells derived from fetal lung tissue on the differentiation of cord blood mononuclear cells into megakaryocyte. The amplification system of heparin was divided into two groups. The first group was cultured alone with CD34 ~ cells and the second group co-cultured with mesenchymal stem cells. The living cells were counted and the expression of CD41a and CD61 were analyzed by flow cytometry. The morphology and structure of the cells were analyzed by immunohistochemistry and transmission electron microscopy on the 14th day. Results the positive rates of CD41a and CD61 were significantly increased after induced by cytokines. Compared with 0 days, the positive rates of CD41a and CD61 were significantly increased after 14 days of culture. CD41a~ cells were amplified by an average of 429.5 times and CD61- cells by 339 times on average. In the second group, the number of CD41 ~ cells and CD61 ~ cells were the highest at 10 days. The results of immunohistochemistry and electron microscope showed that the nuclei of the two groups were immature. In the system of heparin amplification, a large number of megakaryocytes could be induced by using MNC as the starting cells, while fetal lung mesenchymal stem cells could effectively stimulate the generation of CD41 ~ cells and CD61 ~ cells. Effect on nuclear development.
【學(xué)位授予單位】：中國協(xié)和醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2009
【分類號】：R329

【引證文獻】

相關(guān)期刊論文 前1條

1 李霞云;侯玲玲;馬月輝;李向臣;王開功;關(guān)偉軍;;牛胎肺間充質(zhì)干細胞體外培養(yǎng)及生物學(xué)特性研究[J];安徽農(nóng)業(yè)科學(xué);2011年17期

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本文編號：1384129