本文關(guān)鍵詞：運(yùn)動(dòng)及心理應(yīng)激對(duì)大鼠不同部位SOD活性的影響　出處：《吉林大學(xué)》2009年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 運(yùn)動(dòng)負(fù)荷 心理應(yīng)激 超氧化歧化酶(SOD)

【摘要】： 有關(guān)氧自由基的理論一直是運(yùn)動(dòng)醫(yī)學(xué)領(lǐng)域研究的熱點(diǎn)問(wèn)題。所謂氧自由基,就是當(dāng)機(jī)體進(jìn)行代謝時(shí),能奪去氧的一個(gè)電子,這個(gè)氧原子就變成自由基。自由基作為人體垃圾,能夠促使某些疾病的發(fā)生和機(jī)體的衰老。超氧化歧化酶又稱過(guò)氧化物歧化酶(Superoxide Dismutase, SOD) ,這種活性酶能專一地清除體內(nèi)有害的自由基,以解除自由基氧化體內(nèi)的某些組成成分而造成的機(jī)體損害。大量現(xiàn)代醫(yī)學(xué)研究證明,SOD是人體內(nèi)的一種最重要的細(xì)胞保護(hù)酶,既能防輻射損傷,又能有效地抵抗過(guò)氧化陰離子自由基,從而延緩衰老,調(diào)節(jié)機(jī)體代謝能力,提高人體自身的免疫功能。但是,究竟何種運(yùn)動(dòng)強(qiáng)度會(huì)使機(jī)體在運(yùn)動(dòng)后抗氧化能力增強(qiáng),還有待于進(jìn)一步研究。本實(shí)驗(yàn)通過(guò)對(duì)大鼠進(jìn)行不同強(qiáng)度的運(yùn)動(dòng)訓(xùn)練及心理應(yīng)激干預(yù),探討不同的運(yùn)動(dòng)負(fù)荷及心理應(yīng)激對(duì)大鼠機(jī)體內(nèi)SOD的抗氧化能力的影響。 實(shí)驗(yàn)采用游泳方式對(duì)體重為350g-400g的大鼠進(jìn)行中等強(qiáng)度(每天訓(xùn)練30min)、大強(qiáng)度(每天訓(xùn)練60min)、大強(qiáng)度心理應(yīng)激(每天在容器內(nèi)進(jìn)行刺激后運(yùn)動(dòng)60min)的運(yùn)動(dòng)訓(xùn)練,訓(xùn)練8周后處死。取大鼠腹腔動(dòng)脈血,離心后提取血清,肝臟、腎臟、心臟組織制取組織勻漿,采用比色法(試劑盒)測(cè)定各組大鼠血清及組織樣本中的SOD。 結(jié)果表明:不同運(yùn)動(dòng)強(qiáng)度和心理應(yīng)激對(duì)大鼠機(jī)體抗氧化能力產(chǎn)生不同的影響。與對(duì)照組比較,中等強(qiáng)度、大強(qiáng)度、大強(qiáng)度運(yùn)動(dòng)加心理應(yīng)激干預(yù)使機(jī)體的抗氧化能力均提高;但在不同組織內(nèi)的SOD水平變化不同。
[Abstract]:The oxygen free radical theory is always a hot topic in the field of sports medicine. The oxygen free radical, is when the body for metabolism, oxygen can take an electron, the oxygen atoms become free radicals. The free radicals as human waste, can promote the occurrence of certain diseases and the aging body. Superoxide dismutase and superoxide dismutase (Superoxide Dismutase, SOD), the enzyme activity can only remove the body of harmful free radicals, the damage to remove certain components of the body caused by free radical oxidation. Studies show that lots of modern medicine, SOD is one of the most important protective enzyme in human body the both radiation damage, and can effectively resist the superoxide anion radical, thus delaying senescence, regulating body metabolism, improve the body's own immune function. However, what kind of strength will make the machine movement Further study is needed to improve the antioxidant capacity of the body after exercise. In this experiment, rats were subjected to different intensity of exercise training and psychological stress intervention to explore the effects of different exercise loads and psychological stress on the antioxidant capacity of SOD in rats.
The swimming on body weight of 350g-400g rats with moderate intensity (training day 30min), high intensity (training day 60min), high intensity and psychological stress (exercise stimulation in the container every day after 60min) training, training 8 weeks later. The rats abdominal cavity blood after centrifugal extraction serum, liver, kidney, heart tissue homogenate preparation, using colorimetric method (Kit) for determination of serum and tissue samples of SOD. rats
The results show that the different intensity of exercise and psychological stress have different effects on antioxidant capacity in rats. Compared with control group, moderate intensity, high intensity, high intensity exercise intervention psychological stress increases the body's antioxidant capacity were improved; but in different tissues SOD level changes are different.

【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2009
【分類號(hào)】：R363

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