本文關鍵詞：CXCL16轉基因小鼠模型的建立及血脂水平變化的研究　出處：《南華大學》2010年碩士論文　論文類型：學位論文

  更多相關文章： CXCL16 轉基因小鼠 血脂 動脈粥樣硬化

【摘要】： 目的建立CXCL16(結合磷脂酰絲氨酸和氧化型脂蛋白的清道夫受體)轉基因小鼠模型,并觀察其血脂水平的變化。 方法構建帶Flag分子標簽的真核表達載體CXCL16-trans,顯微注射入C57BL"6J小鼠受精卵原核,導入假孕母鼠輸卵管后培養(yǎng)子代小鼠。通過PCR法及Western Blot鑒定小鼠基因型,獲得CXCL16轉基因小鼠模型(CXCL16 transgenic mouse ,CXCL16 trans )。8周齡F5代CXCL16 trans鼠和野生型小鼠(C57BL"6J)各10只,經12周高脂飼料喂養(yǎng)后,應用紫外分光儀法檢測喂養(yǎng)前后小鼠的總膽固醇、甘油三脂、低密度脂蛋白及高密度脂蛋白的水平。 結果1.構建攜帶分子標簽Flag的表達載體CXCLl16-trans質粒經酶切與測序,鑒定序列正確。在171只原代小鼠中,有26只轉基因陽性Founder小鼠。目前F5代71只小鼠中,有6系共31只轉基因陽性小鼠。 2.CXCL16 trans與野生型小鼠經12周高脂飼料喂養(yǎng)后,前者血清膽固醇、甘油三酯及低密度脂蛋白水平明顯高于后者,而血清高密度脂蛋白水平低于后者。 結論1.成功建立攜帶分子標簽Flag的CXCL16轉基因小鼠模型。 2.高脂飼料喂養(yǎng)的CXCL16轉基因小鼠,其血清總膽固醇、甘油三酯及低密度脂蛋白明顯增高,而血清高密度脂蛋白水平明顯降低,提示基因CXCL16在動脈粥樣硬化的發(fā)生發(fā)展過程中可能起著促進的作用。
[Abstract]:Objective to establish a CXCL16 transgenic mouse model with phosphatidyl serine and oxidized lipoprotein scavenger receptor, and to observe the changes of lipid levels in CXCL16 transgenic mice. Methods Eukaryotic expression vector CXCL16-transcoding with Flag molecular label was constructed and microinjected into prokaryotic of C57BL "6J mouse fertilized egg." The progeny mice were cultured in the fallopian tubes of pseudopregnant mice. The genotypes of the mice were identified by PCR and Western Blot. CXCL16 transgenic mouse model CXCL16 transgenic mouse was obtained. There were 10 CXCL16 trans mice of F5 generation and 10 C57BL "6J" mice of CXCL16 trans at the age of 8 weeks. The mice were fed with high fat diet for 12 weeks. The levels of total cholesterol, triglyceride, low density lipoprotein and high density lipoprotein in mice before and after feeding were detected by ultraviolet spectrometer. The expression vector CXCLl16-trans plasmid carrying molecular label Flag was constructed by restriction endonuclease digestion and sequencing. The sequence was correct in 171 primary mice. 2. There were 26 transgenic Founder mice and 31 transgenic mice in 6 lines out of 71 F5 generation mice. 2. The levels of serum cholesterol, triglyceride and low density lipoprotein in CXCL16 trans and wild-type mice were significantly higher than those in wild type mice. The serum HDL level was lower than that of the latter. The model of CXCL16 transgenic mice carrying molecular label Flag was successfully established. 2. 2.The serum total cholesterol, triglyceride and low density lipoprotein of CXCL16 transgenic mice fed with high fat diet were significantly increased, while the serum high density lipoprotein level was obviously decreased. It is suggested that gene CXCL16 may play a promoting role in the development of atherosclerosis.
【學位授予單位】：南華大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R-332

【引證文獻】

相關碩士學位論文 前1條

1 董一寰;過表達CXCL16的轉基因小鼠血清MCP-1和ICAM-1水平的變化[D];南華大學;2013年

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本文編號：1363546