【摘要】：目的研究全反式維甲酸(ATRA)對成年小鼠神經(jīng)干細胞(NSCs)的調(diào)控、信號通路及對細胞色素P450(CYP450)家族的影響。方法分離并培養(yǎng)成年小鼠腦室下區(qū)NSCs,將細胞分為ATRA組和對照組,ATRA組加入10-6mol/L ATRA,對照組正常培養(yǎng)。采用流式細胞儀檢測兩組細胞表面標(biāo)記物,計算神經(jīng)元、星形膠質(zhì)細胞、少突膠質(zhì)細胞和NSCs占總細胞數(shù)的百分比;Real-time PCR方法檢測CYP450家族相關(guān)基因表達情況,篩選出有統(tǒng)計學(xué)意義的基因;ELISA法測定細胞色素P450還原酶(CPR)的活性;Western blotting法檢測P38 MAPK通路相關(guān)蛋白P38、p-P38蛋白的相對表達量。結(jié)果 ATRA組NSCs主要是向神經(jīng)元分化,也有一部分向星形膠質(zhì)細胞分化,少突膠質(zhì)細胞較少。與對照組比較,ATRA組P450家族基因中CYP26A1、CYP26B1、CYP26C1表達上調(diào)(P均0.05)。ATRA組CPR活性以及p-P38蛋白表達量均較對照組升高(P均0.05)。結(jié)論 ATRA促進小鼠NSCs向神經(jīng)元分化,可能通過上調(diào)CYP450中的CYP26家族基因、增加CPR活性以及P38 MAPK通路發(fā)揮調(diào)控作用。
[Abstract]:Objective to study the regulation, signaling pathway and cytochrome P450 (CYP450) family of adult mouse neural stem cells (NSCs) by all-trans retinoic acid (ATRA). Methods NSCs in the subventricular region of adult mice were isolated and cultured. The cells were divided into ATRA group and control group and 10-6 mol / L ATRA was added into the control group. Cell surface markers were detected by flow cytometry, and the percentage of neurons, astrocytes, oligodendrocytes and NSCs in total cells were calculated. Real-time PCR was used to detect the expression of CYP450 family related genes. The activity of cytochrome P450 reductase (CPR) was detected by Elisa. Western blotting was used to detect the relative expression of P38 MAPK pathway related protein P38 p-P38. Results in ATRA group, NSCs were mainly differentiated into neurons, and some of them were differentiated into astrocytes, with less oligodendrocytes. Compared with the control group, the expression of CYP26A1C1-CYP26B1-CYP26C1 was up-regulated in ATRA group (P 0.05). The CPR activity and p-P38 protein expression in ATRA group were higher than those in control group (P 0.05). Conclusion ATRA can promote the differentiation of NSCs into neurons, which may play a regulatory role by up-regulating CYP26 family gene in CYP450, increasing CPR activity and P38 MAPK pathway.
【作者單位】： 蘇州大學(xué)附屬常州第一人民醫(yī)院;蘇州衛(wèi)生職業(yè)技術(shù)學(xué)院;徐州醫(yī)科大學(xué);
【基金】：江蘇省自然科學(xué)基金資助項目(BK20130219) 江蘇高�！扒嗨{工程”科研資助項目
【分類號】：R741

【相似文獻】

相關(guān)期刊論文 前10條

1 徐迎勝,樊東升;神經(jīng)干細胞及其臨床應(yīng)用前景[J];神經(jīng)疾病與精神衛(wèi)生;2002年01期

2 戴宜武;神經(jīng)干細胞發(fā)育過程中分化、遷移調(diào)控機制研究現(xiàn)狀[J];中華神經(jīng)醫(yī)學(xué)雜志;2003年02期

3 黃毓;神經(jīng)干細胞的研究進展[J];廣西醫(yī)科大學(xué)學(xué)報;2004年02期

4 高小青,袁瓊蘭;神經(jīng)干細胞的分離及培養(yǎng)方法[J];瀘州醫(yī)學(xué)院學(xué)報;2004年04期

5 王心;神經(jīng)干細胞(Neural stem cells)[J];日本醫(yī)學(xué)介紹;2005年07期

6 安沂華;程洪斌;張儒有;張贊;李紀(jì)仲;;神經(jīng)干細胞臨床應(yīng)用和前景展望[J];內(nèi)科急危重癥雜志;2005年05期

7 陳文提;;神經(jīng)干細胞在中樞神經(jīng)系統(tǒng)疾病治療的研究進展[J];福建醫(yī)藥雜志;2007年02期

8 田增民;孫君昭;;神經(jīng)干細胞應(yīng)用研究進展[J];中國實用內(nèi)科雜志;2007年10期

9 韋巖;吳琪;;神經(jīng)干細胞的研究進展[J];菏澤醫(yī)學(xué)�？茖W(xué)校學(xué)報;2007年03期

10 王煥明;徐如祥;;神經(jīng)干細胞與顳葉癲沲[J];臨床神經(jīng)外科雜志;2008年03期

相關(guān)會議論文 前10條

1 劉佩;劉U，

本文編號：2136181