支配牙髓的三叉神經(jīng)分支神經(jīng)胞體在三叉神經(jīng)節(jié)內(nèi)的區(qū)域分布
發(fā)布時間:2018-06-18 21:13
本文選題:三叉神經(jīng)節(jié) + 三叉神經(jīng)痛。 參考:《安徽醫(yī)科大學(xué)》2014年碩士論文
【摘要】:目的觀察正常及三叉神經(jīng)疼痛模型大鼠支配牙髓的三叉神經(jīng)分支的神經(jīng)胞體在三叉神經(jīng)節(jié)內(nèi)的區(qū)域分布情況,了解正常及三叉神經(jīng)痛模型大鼠牙髓神經(jīng)胞體在三叉神經(jīng)節(jié)內(nèi)與被支配牙髓的定位關(guān)系,為臨床通過三叉神經(jīng)節(jié)處理治療三叉神經(jīng)痛提供解剖學(xué)證據(jù)。 方法通過在正常大鼠(假手術(shù)組)以及眶下神經(jīng)慢性縮窄性損傷(三叉神經(jīng)痛模型)大鼠(模型組)的不同牙髓腔內(nèi)(左側(cè)上中切牙牙髓、右側(cè)下中切牙牙髓、左側(cè)上中切牙牙髓和左側(cè)下頜第一磨牙牙髓)注入逆行熒光探針(Fast DiO和/或Fast DiI),72h后用熒光顯微鏡觀察各組大鼠三叉神經(jīng)節(jié)中的被熒光標(biāo)記的神經(jīng)胞體分布情況。 結(jié)果正常大鼠的右下側(cè)中切牙注入熒光探針Fast DiO72h后,,在其同側(cè)三叉神經(jīng)節(jié)的后外側(cè)區(qū)域可以觀察到有少量綠色熒光標(biāo)記的神經(jīng)元,約3-5個,成簇分布。正常大鼠的左上側(cè)中切牙注入熒光探針Fast DiO72h后,在其同側(cè)三叉神經(jīng)節(jié)的中間區(qū)域段可以觀察到綠色熒光標(biāo)記的神經(jīng)元,約數(shù)個,也成簇分布。正常大鼠右側(cè)下中切牙及左側(cè)上中切牙髓腔分別注射熒光探針Fast DiO和Fast DiI,72h后發(fā)現(xiàn)在其右側(cè)三叉神經(jīng)節(jié)的后外側(cè)區(qū)域及左側(cè)三叉神經(jīng)節(jié)的中間區(qū)域可分別觀察到標(biāo)記的神經(jīng)元。正常大鼠左側(cè)上中切牙及左側(cè)下頜第一磨牙髓腔分別注射熒光探針Fast DiI和Fast DiO,72h后發(fā)現(xiàn)在其左側(cè)三叉神經(jīng)節(jié)的中后段區(qū)域及后外側(cè)區(qū)域可觀察到少量成簇分布的紅色和綠色熒光標(biāo)記的神經(jīng)元。以上所有實驗觀察在其對側(cè)三叉神經(jīng)節(jié)相應(yīng)位置以及其它部位均未見有熒光標(biāo)記的神經(jīng)元。在三叉神經(jīng)疼痛模型大鼠的相應(yīng)的牙髓腔內(nèi)注入相應(yīng)的熒光標(biāo)記物后,在三叉神經(jīng)節(jié)內(nèi)檢測到帶有熒光的神經(jīng)元分布及熒光數(shù)量和強度沒有明顯的變化。 結(jié)論支配牙髓的三叉神經(jīng)的各分支胞體在三叉神經(jīng)節(jié)內(nèi)的分布具有一定的區(qū)域性,且為單側(cè)性投射;三叉神經(jīng)痛發(fā)生后并不影響三叉神經(jīng)節(jié)內(nèi)神經(jīng)元的數(shù)量以及投射聯(lián)系,三叉神經(jīng)痛的發(fā)生可能與三叉神經(jīng)元的功能活動改變有關(guān)。
[Abstract]:Objective to observe the distribution of nerve cell bodies in trigeminal ganglion of normal and trigeminal pain rats. Objective to investigate the relationship between the localization of dental pulp neuronal bodies in the trigeminal ganglion and the innervated pulp in normal and trigeminal neuralgia rats, and to provide anatomic evidence for the treatment of trigeminal neuralgia through the treatment of trigeminal ganglion. Methods the pulp of left superior incisor and right inferior incisor of rats with chronic constrictive injury of suborbital nerve (trigeminal neuralgia model) were studied in normal rats (sham-operated group) and rats (model group). The retrograde fluorescent probe Fast Dio and / or Fast DiI were injected into the pulp of left central incisor and left mandibular first molar for 72 hours. The distribution of fluorescent labeled neuronal bodies in the trigeminal ganglion of rats in each group was observed by fluorescence microscope. Results in the ipsilateral trigeminal ganglion, a small number of green fluorescent labeled neurons were observed in the ipsilateral trigeminal ganglion, about 3-5 neurons in clusters, after the injection of fluorescent probe Fast Dio into the right lower central incisor of normal rats. Green fluorescent labeled neurons were observed in the middle region of the ipsilateral trigeminal ganglion after fluorescent probe Fast Dio was injected into the left upper incisor of normal rats. The labeled neurons were observed in the posterolateral region of the right trigeminal ganglion and the middle region of the left trigeminal ganglion in the right inferior incisor and the left superior incisor respectively 72 h after the injection of the fluorescent probes Fast Dio and Fast DiI respectively into the pulp cavity of the right inferior incisor and the left superior incisor of the normal rat. Fluorescence probes Fast Dii and Fast Dio were injected into the pulp cavity of left upper incisor and left mandibular first molar in normal rats for 72 hours, respectively. A few clusters of red were observed in the middle and posterior segment of left trigeminal ganglion and posterolateral region of left trigeminal ganglia. Colored and green fluorescent labeled neurons. No fluorescent labeled neurons were found in the contralateral trigeminal ganglion. The distribution of fluorescent neurons, the quantity and intensity of fluorescence in trigeminal ganglion were not significantly changed after the corresponding fluorescent markers were injected into the dental pulp cavity of the rats with trigeminal nerve pain. Conclusion the distribution of the branches of trigeminal nerve in the trigeminal ganglion is regional and unilateral, and the number of neurons in trigeminal ganglion is not affected after the occurrence of trigeminal neuralgia. The occurrence of trigeminal neuralgia may be related to the functional changes of trigeminal neurons.
【學(xué)位授予單位】:安徽醫(yī)科大學(xué)
【學(xué)位級別】:碩士
【學(xué)位授予年份】:2014
【分類號】:R745.11
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