本文選題：腦積水 + 高嶺土��； 參考：《上海交通大學(xué)學(xué)報(醫(yī)學(xué)版)》2017年10期

【摘要】：目的·觀察半胱氨酸組織蛋白酶S(CatS)基因敲除對高嶺土誘導(dǎo)小鼠交通性腦積水嚴重程度的影響。方法·針對8只CatS基因敲除(CatS~(-/-))小鼠和16只野生型(WT)C57BL/6小鼠,通過枕大池注射高嶺土混懸液的方法建立交通性腦積水模型,建模前及建模后1周利用頭顱磁共振(MRI)掃描測量小鼠側(cè)腦室體積并計算側(cè)腦室指數(shù),比較2組小鼠腦積水嚴重程度。結(jié)果·建模后1周CatS~(-/-)組死亡1只(12.5%),WT組死亡2只(12.5%),2組間死亡率的差異無統(tǒng)計學(xué)意義(P=1.000);頭顱MRI結(jié)果顯示存活CatS~(-/-)組和WT組小鼠均有不同程度腦室擴大,建模前CatS~(-/-)組(n=8)側(cè)腦室指數(shù)為0.05±0.01,WT組(n=16)側(cè)腦室指數(shù)為0.04±0.01(P=0.720)。建模后1周CatS~(-/-)組(n=7)側(cè)腦室指數(shù)為0.13±0.02,其中側(cè)腦室指數(shù)增加1倍以上者5只,占71.4%;WT組(n=14)側(cè)腦室指數(shù)為0.11±0.01,側(cè)腦室指數(shù)增加1倍以上者10只,占71.4%;2組比較,差異無統(tǒng)計學(xué)意義(P=0.950)。結(jié)論·枕大池注射高嶺土混懸液后1周,小鼠側(cè)腦室明顯擴大伴腦積水發(fā)生,模型成功率高;CatS基因敲除對高嶺土誘導(dǎo)小鼠腦交通性腦積水發(fā)展無明顯影響。
[Abstract]:Objective to observe the effect of cysteine cathepsin Sch CatS knockout on the severity of communicating hydrocephalus induced by kaolin in mice. Methods the model of communicating hydrocephalus was established by injecting kaolin suspension into occipital cistern in 8 CatS knockout mice and 16 wild type WTA C57BL / 6 mice. The volume of lateral ventricle was measured and the index of lateral ventricle was calculated before and 1 week after modeling. The severity of hydrocephalus was compared between the two groups. Results one week after modeling, there was no significant difference in the mortality rate between the two groups in the WT group and the WT group, but there was no significant difference in the mortality rate between the two groups, and the cranial MRI results showed that both the surviving CatS group and WT group had different degrees of ventricular dilatation, and the results showed that there was no significant difference between the two groups in the mortality of the two groups, and the results of cranial MRI showed that both the survival group and the WT group had different degrees of ventricular dilatation. Before modeling, the ventricular index (VI) was 0.05 鹵0.01kW / L) in the Catson-r-R group (0.04 鹵0.01P0. 720) in WT group (P = 0. 720) and 0. 05 鹵0. 01 (P = 0. 720) in the control group (n = 0. 05 鹵0. 01). One week after modeling, the index of lateral ventricle was 0.13 鹵0.02in the CatSti-r-R group (0.13 鹵0.02), in which the index of lateral ventricle increased more than twice in 5 rats, accounting for 71.4% of WT group (0.11 鹵0.01), while the index of lateral ventricle increased more than 1 fold in 10 rats (71.4%). There was no significant difference between the two groups (P = 0.950). Conclusion 1 week after injection of kaolinite suspension into cistern, the lateral ventricle was obviously enlarged with hydrocephalus in mice. The high success rate of CatS gene knockout had no significant effect on the development of cerebral communicating hydrocephalus induced by kaolin in mice.
【作者單位】： 上海交通大學(xué)醫(yī)學(xué)院附屬第九人民醫(yī)院神經(jīng)內(nèi)科;華東師范大學(xué)物理系上海磁共振重點實驗室;浙江中醫(yī)藥大學(xué)附屬杭州市西溪醫(yī)院神經(jīng)內(nèi)科;
【基金】：上海市科學(xué)技術(shù)委員會創(chuàng)新行動計劃基礎(chǔ)研究重點項目(14JC1404300);上海市科學(xué)技術(shù)委員會生物醫(yī)藥支撐項目(16411953100) 上海交通大學(xué)醫(yī)學(xué)院多中心臨床研究項目(DLY201614) 上海交通大學(xué)醫(yī)學(xué)院—中國科學(xué)院神經(jīng)科學(xué)研究所腦疾病臨床研究中心2015年率先啟動項目(2015NKX006) 上海申康醫(yī)院發(fā)展中心臨床科技創(chuàng)新項目(SHDC12015310)~~
【分類號】：R742.7

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