本文選題：鐵調(diào)素 + 蛛網(wǎng)膜下腔出血　； 參考：《重慶醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的：觀察蛛網(wǎng)膜下腔出血（subarachnoid hemorrhage，SAH）后早期大鼠海馬鐵含量及鐵調(diào)素（Hepcidin）蛋白的表達(dá)變化，探討鐵調(diào)素參與SAH后早期腦鐵代謝紊亂的可能機(jī)制。 方法：成年雄性SD大鼠90只，隨機(jī)分為假手術(shù)對(duì)照組，實(shí)驗(yàn)組(蛛網(wǎng)膜下腔出血組)。采用視交叉前池注血法構(gòu)造大鼠SAH模型。在SAH后早期12、24、48、72h采用免疫組化、Western-blot法檢測(cè)大鼠SAH后海馬組織內(nèi)Hepc的蛋白表達(dá)情況，使用原子吸收光譜儀對(duì)海馬組織總鐵含量進(jìn)行檢測(cè)。 結(jié)果：免疫組化實(shí)驗(yàn)結(jié)果發(fā)現(xiàn)，，相對(duì)于假手術(shù)對(duì)照組（0.190.05），大鼠SAH后12h見(jiàn)Hepcidin表達(dá)水平明顯增高（0.300.06）,至72h呈逐漸增高趨勢(shì)（0.560.07），差異有統(tǒng)計(jì)學(xué)意義（F=31.911，P㩳0.05）；Western blot實(shí)驗(yàn)同樣發(fā)現(xiàn)，與假手術(shù)（對(duì)照）比較（0.238 0.047），大鼠SAH后12h見(jiàn)Hepcidin蛋白表達(dá)水平明顯增高（0.481 0.065）,至72h呈逐漸增高趨勢(shì)（1.6270.143），差異有統(tǒng)計(jì)學(xué)意義（F=147.314，P㩳0.05）；原子吸收光譜儀在蛛網(wǎng)膜下腔出血后12h檢測(cè)到大鼠海馬總鐵含量開(kāi)始增加（58.509.19），至72h呈逐漸增高趨勢(shì)（99.347.68），與假手術(shù)對(duì)照組比較（43.514.59），差異有統(tǒng)計(jì)學(xué)意義（F=28.799,P㩳0.05）；大鼠海馬組織Hepcidin蛋白的表達(dá)量與海馬鐵含量成正相關(guān)（r=0.914，P0.05）。結(jié)論：大鼠SAH后腦鐵含量的增加可能與Hepcidin表達(dá)增高有關(guān)。
[Abstract]:Aim: to observe the changes of iron content in hippocampus and the expression of iron modulin (hepcidin) protein in hippocampus of rats with subarachnoid hemorrhage (SAH) after subarachnoid hemorrhage (SAH), and to explore the possible mechanism of iron modulin involved in early brain iron metabolism disorder after SAH. Methods: 90 adult male SD rats were randomly divided into sham operation control group and experimental group (subarachnoid hemorrhage group). The rat SAH model was constructed by injecting blood into the anterior cistern of optic chiasma. The expression of Hepc protein in hippocampus of rats with SAH was detected by immunohistochemistry at 12: 24, 48 and 72 hours after SAH, and the total iron content of hippocampus was detected by atomic absorption spectrometer (AAS). Results: compared with the sham-operated control group (0.190.05), the expression of Hepcidin increased significantly 12 hours after SAH, and gradually increased to 0.560.07 at 72 h after SAH. The difference was statistically significant. Compared with sham-operated (control) 0.238 The expression of Hepcidin protein was significantly increased in rats 12 hours after SAH. The content of total iron in hippocampus of rats was detected by atomic absorption spectrometer at 12 hours after subarachnoid hemorrhage, and the total iron content in hippocampus of rats began to increase by 58.509.19 and 99.347.68% at 72 hours, which was significantly higher than that of sham operation control group (P < 0.05). The content of total iron in hippocampus of rats was increased gradually at 72 h after SAH by atomic absorption spectrometer (AAS), and the content of total iron in hippocampus of rats was increased by atomic absorption spectrometer (AAS) at 12 hours after subarachnoid hemorrhage. Compared with 43.514.59, the difference was statistically significant, and the expression of Hepcidin protein in rat hippocampus was positively correlated with the content of iron in hippocampus. Conclusion: the increase of brain iron content after SAH may be related to the increase of Hepcidin expression.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R743.35

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 宋捷;宋寧;姜宏;;6-羥基多巴胺對(duì)星形膠質(zhì)細(xì)胞hepcidin表達(dá)的影響[J];青島大學(xué)醫(yī)學(xué)院學(xué)報(bào);2012年05期

2 李艷偉;趙晉英;周澤江;黃澤智;李琳;;海馬注射β淀粉樣蛋白1-40對(duì)大鼠腦鐵含量及膜鐵轉(zhuǎn)運(yùn)蛋白表達(dá)的影響[J];廣東醫(yī)學(xué);2013年11期

3 劉樹(shù)欣;劉玉倩;王海濤;秦春;梁國(guó)彬;趙斌;;鐵調(diào)素(Hepcidin)對(duì)細(xì)胞鐵代謝影響的研究進(jìn)展[J];河北師范大學(xué)學(xué)報(bào)(自然科學(xué)版);2011年01期

4 閆芬;李穎;劉樹(shù)欣;劉玉倩;;運(yùn)動(dòng)引起的白細(xì)胞介素6的改變與鐵調(diào)素的調(diào)控[J];河北師范大學(xué)學(xué)報(bào)(自然科學(xué)版);2011年03期

5 蔣士生;韓育明;賀雙騰;;腦出血后腦內(nèi)游離血紅素對(duì)神經(jīng)元的損傷作用及機(jī)理研究概況[J];湖南中醫(yī)雜志;2011年04期

6 劉超;閔蘇;;慢性心理應(yīng)激導(dǎo)致的鐵代謝障礙與記憶損害[J];臨床麻醉學(xué)雜志;2012年02期

7 李艷偉;周澤江;趙晉英;李琳;;膜鐵轉(zhuǎn)運(yùn)蛋白1與腦鐵代謝研究進(jìn)展[J];神經(jīng)解剖學(xué)雜志;2011年04期

8 ;Sodium glutamate and gamma-aminobutyric acid affect iron metabolism in the rat caudate putamen[J];Neural Regeneration Research;2010年21期

9 劉樹(shù)欣;王海濤;劉玉倩;問(wèn)亞飛;程康康;李海洲;蔡琳琳;;運(yùn)動(dòng)性低血色素形成過(guò)程中大鼠肝臟HJV mRNA表達(dá)及血清Hepcidin含量的變化[J];體育科學(xué);2011年03期

10 王福O

本文編號(hào)：1906505