本文選題：依達(dá)拉奉注射液 + 單唾液酸神經(jīng)節(jié)苷脂注射液�。� 參考：《中國(guó)臨床藥理學(xué)雜志》2017年11期

【摘要】：目的研究單唾液酸神經(jīng)節(jié)苷脂聯(lián)合依達(dá)拉奉對(duì)抗腦缺血性神經(jīng)細(xì)胞損傷的作用。方法選取健康SD大鼠,按照體重隨機(jī)分為模型組和實(shí)驗(yàn)組,每組21只。實(shí)驗(yàn)組:腹腔注射單唾液酸神經(jīng)節(jié)苷脂20 mg·kg~(-1),每天1次;腹腔注射依達(dá)拉奉3 mg·kg~(-1),每天2次。模型組,腹腔注射等量1%的0.9%Na Cl。均給藥14 d。用大腦中動(dòng)脈阻塞線栓法制作局灶性腦缺血再灌注動(dòng)物模型。用圖像分析系統(tǒng),依據(jù)大鼠腦缺血半暗袋定位,在相同的視野下,將大腦中動(dòng)脈阻塞的同側(cè)額頂葉皮質(zhì)上部界定為半暗帶的等值觀察區(qū),用免疫組化法對(duì)大鼠絲氨酸-蘇氨酸蛋白激酶(Akt)、糖原合酶激酶-3(GSK-3β)、3-磷酸肌醇依賴性蛋白激酶(PDK1)及磷脂酰肌醇3激酶(PI3K)的蛋白免疫反應(yīng)陽(yáng)性細(xì)胞平均光密度與陽(yáng)性面積單位進(jìn)行對(duì)比分析。結(jié)果模型組和實(shí)驗(yàn)組PI3K蛋白、Akt蛋白及PDK1蛋白的表達(dá)均加強(qiáng)、而GSK3β蛋白表達(dá)均減弱。7 d后,PI3K蛋白、Akt蛋白、PDK1蛋白及GSK3β蛋白的陽(yáng)性單位面積,模型組與實(shí)驗(yàn)組分別為(19.58±1.13),(26.91±0.90)mm~2;(15.98±0.48),(22.87±1.20)mm~2;(17.97±0.58),(26.02±0.54)mm~2;(22.03±0.92),(14.02±0.45)mm~2,組間對(duì)比差異均有統(tǒng)計(jì)學(xué)意義(均P0.05)。7 d后這4種蛋白的平均光密度值,模型組與實(shí)驗(yàn)組分別為0.19±0.01,0.28±0.02;0.16±0.01,0.25±0.01;0.16±0.01,0.25±0.01;0.22±0.01,0.14±0.01,組間對(duì)比差異均有統(tǒng)計(jì)學(xué)意義(均P0.05)。結(jié)論單唾液酸神經(jīng)節(jié)苷脂結(jié)合依達(dá)拉奉可抵抗腦缺血區(qū)域神經(jīng)細(xì)胞的損傷。
[Abstract]:Objective to study the effect of monoglycoside combined with Edaravone on cerebral ischemic nerve cell injury. Methods healthy Sprague-Dawley rats were randomly divided into model group and experimental group according to body weight with 21 rats in each group. Experimental group: single sialic acid ganglioside was injected intraperitoneally, once a day, and Edaravone 3 mg / kg, twice a day. In the model group, 1% 0.9%Na Cl was injected intraperitoneally. The drug was given for 14 days. The animal model of focal cerebral ischemia reperfusion was established by middle cerebral artery occlusion. Using an image analysis system, the middle cerebral artery occlusion of the upper frontal parietal cortex of the middle cerebral artery was defined as the equivalent observation area of the penumbra under the same field of vision according to the localization of the cerebral ischemic semi-dark bag in rats. The average optical density and positive surface of protein immunoreactive cells of rat serine threonine protein kinase, glycogen synthase kinase GSK-3 尾 -phosphoinositol dependent protein kinase PDK1 and phosphatidylinositol 3 kinase PI3K were determined by immunohistochemical method. The unit of product is compared and analyzed. Results the expression of PI3K protein and PDK1 protein were enhanced in model group and experimental group, while the expression of GSK3 尾 protein was decreased at 7. 7 d after the expression of GSK3 尾 protein, the positive unit area of PDK1 protein and GSK3 尾 protein of PI3K protein was decreased. 妯″瀷緇勪笌瀹為獙緇勫垎鍒負(fù)(19.58鹵1.13),(26.91鹵0.90)mm~2;(15.98鹵0.48),(22.87鹵1.20)mm~2;(17.97鹵0.58),(26.02鹵0.54)mm~2;(22.03鹵0.92),(14.02鹵0.45)mm~2,緇勯棿瀵規(guī)瘮宸紓鍧囨湁緇熻瀛︽剰涔，

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