本文選題：實驗性自身免疫性腦脊髓炎 + 多發(fā)性硬化　； 參考：《遼寧中醫(yī)雜志》2017年09期

【摘要】：目的:探索實驗性自身免疫性腦脊髓炎(EAE)小鼠中樞神經系統(tǒng)不同部位間的病理變化,為疾病機制探索及治療研究提供實驗取材依據。方法:采用PLP139-151和百日咳毒素誘導SJL小鼠建立EAE模型,至發(fā)病高峰期分別取小鼠的大腦、小腦、腦干、頸髓、胸髓、腰髓進行HE染色及LFB髓鞘染色觀察,比較各部位組織病理變化和髓鞘脫失情況。結果:腦干和大腦的病理變化顯著,具有明顯的血管袖套樣炎性細胞浸潤,小腦病理變化較小。三段脊髓均呈現出明顯的病理變化,并以頸段和腰段最顯著,出現明顯的炎性細胞浸潤和神經元細胞空泡狀壞死。結論:在評判SJL小鼠EAE模型的病理特征時,腦干、大腦、頸髓和腰髓是最具特征的病變部位。
[Abstract]:Objective: to explore the pathological changes of central nervous system (CNS) in experimental autoimmune encephalomyelitis (EAE) mice.Methods: EAE model was established in SJL mice induced by PLP139-151 and pertussis toxin. The brain, cerebellum, brain stem, cervical spinal cord, thoracic spinal cord and lumbar spinal cord were stained by HE and LFB myelin sheath staining respectively.Histopathological changes and demyelination of myelin were compared.Results: the pathological changes of brain stem and cerebrum were significant, with obvious vascular cuff inflammatory cell infiltration, and the cerebellar pathological change was small.All the three segments of spinal cord showed obvious pathological changes, and the cervical and lumbar segments were the most prominent, with obvious inflammatory cell infiltration and vacuolated necrosis of neuron cells.Conclusion: brain stem, brain, cervical spinal cord and lumbar spinal cord are the most characteristic pathological features of EAE model in SJL mice.
【作者單位】： 西南交通大學生命科學與工程學院;中國中醫(yī)科學院中醫(yī)臨床基礎醫(yī)學研究所;香港浸會大學中醫(yī)藥學院;
【基金】：國家自然科學基金(81470072)
【分類號】：R-332;R744.5
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本文編號：1745248