本文選題：特應(yīng)性皮炎　切入點：microRNA　出處：《瀘州醫(yī)學院》2012年碩士論文

【摘要】：目的：檢測特應(yīng)性皮炎(Atopic dermatitis, AD)患者外周血單個核細胞（Peripheral Blood Mononuclear Cells, PBMCs）中miR-155和miR-142-5p的表達情況，，為進一步研究miR-155、miR-142-5p在特應(yīng)性皮炎發(fā)生、發(fā)展中的作用提供理論依據(jù)。材料與方法：收集8例AD患者及6例正常人外周靜脈血各5ml，人淋巴細胞分離液分離PBMCs，miRNA提取分離試劑盒提取分離PBMCs中的miRNA,采用實時熒光定量PCR法，檢測AD患者和正常人PBMCs中miR-155、miR-142-5p的表達水平。以U6snRNA為內(nèi)參，采用2-ΔΔCT法計算miR-155、miR-142-5p在AD患者PBMCs中的相對表達量，分析AD的嚴重程度（SCORAD）評分與miR-155、miR-142-5p的表達水平之間的關(guān)系。用SPSS17.0統(tǒng)計軟件對實驗數(shù)據(jù)進行統(tǒng)計學分析，以α=0.05作為檢驗標準。結(jié)果：1. miR-155、miR-142-5p在AD患者PBMCs中均有表達。2.與正常對照組相比，miR-155、miR-142-5p在AD患者PBMCs中的表達水平上調(diào)（p＜0.05）。3. miR-155的相對表達量與AD嚴重程度積分有相關(guān)傾向(r=0.695, p=0.056),而miR-142-p的相對表達量與其無相關(guān)性(r=0.283, p=0.497)。結(jié)論：1.特應(yīng)性皮炎患者PBMCs中存在miR-155、miR-142-5P，并可以用實時熒光定量PCR進行檢測。2. miRNA-155、miRNA-142-5p在AD患者PBMCs中表達上調(diào)，可能在AD的發(fā)生、發(fā)展過程中發(fā)揮一定作用。3. miR-155的相對表達量與AD嚴重程度積分有相關(guān)傾向,而miR-142-p的相對表達量與其無相關(guān)性。
[Abstract]:Objective: to detect the expression of miR-155 and miR-142-5p in peripheral Blood Mononuclear cells (PBMCs) in patients with atopic dermatitis (AD), in order to further study the occurrence of miR-155 miR-142-5p in atopic dermatitis. Materials and methods: 5 ml of peripheral venous blood were collected from 8 patients with AD and 6 cases of normal controls. The isolation kit was used to extract miRNAs from peripheral blood of 8 patients with AD and 6 normal controls. Real time fluorescence quantitative PCR method was used to extract miRNAs from PBMCs. The expression of miR-155 miR-142-5p was detected in AD patients and normal subjects, and the relative expression of miR-155 miR-142-5p in PBMCs of AD patients was calculated by 2- 螖 CT using U6snRNA as internal control. The relationship between the severity of AD and the expression level of miR-155 miR-142-5p was analyzed. The experimental data were statistically analyzed by SPSS17.0 software. Results: 1. MiR-155 miR-142-5p was expressed in PBMCs of AD patients. The expression level of miR-155 miR-142-5p was up-regulated in PBMCs of AD patients compared with normal controls. The relative expression of miR-155 was significantly correlated with the score of AD severity. Conclusion: 1. MiR-155 miR-142-5P exists in PBMCs of patients with atopic dermatitis, and can be detected by real-time fluorescence quantitative PCR to detect upregulation of .2miRNA-155 miRNA-142-5p in PBMCs of AD patients. It may play a certain role in the occurrence and development of AD. The relative expression of miR-155 has a tendency to correlate with the score of AD severity, but the relative expression of miR-142-p has no correlation with AD.
【學位授予單位】：瀘州醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R758.2

【參考文獻】

相關(guān)期刊論文 前7條

1 唐勇;吳吉芳;;特應(yīng)性皮炎患者外周血細胞亞群DC1/DC2、Th1/Th2檢測的臨床意義[J];標記免疫分析與臨床;2009年05期

2 楊軍;李成榮;王國兵;黃惠君;祖穎;李永柏;;Th17細胞與CD4~+CD25~+調(diào)節(jié)性T細胞在兒童過敏性紫癜發(fā)病機制中的作用[J];臨床兒科雜志;2009年07期

3 黨鑫堂;伍昌林;張愛根;;特應(yīng)性皮炎患兒外周血Th17、Th1/Th2細胞亞群的研究[J];中國熱帶醫(yī)學;2010年02期

4 趙遐;唐元家;曲波;崔慧娟;王樹軍;王立珈;黃新芳;陳順樂;沈南;;miR-125a調(diào)節(jié)系統(tǒng)性紅斑狼瘡患者T細胞炎癥性趨化因子RANTES的表達[J];現(xiàn)代免疫學;2010年03期

5 李莉;林曉丹;溫旺榮;;莖環(huán)引物的SYBR GreenⅠ實時熒光定量PCR檢測miR-155表達及其臨床初步應(yīng)用[J];檢驗醫(yī)學;2010年04期

6 金培志;楊瑛;趙躍鳳;郭岱炯;;特應(yīng)性皮炎患者Th1/Th2細胞因子譜的檢測及意義[J];中國當代醫(yī)藥;2010年24期

7 劉伏香;譚志琴;龍丹;戈立東;;miR-155在子宮內(nèi)膜癌組織中的表達及其臨床意義[J];中國癌癥雜志;2011年09期

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