【摘要】：目的:觀察茶多酚(green tea polyphenols,GTP)對果糖誘導(dǎo)的高尿酸血癥(hyperuricemia,HUA)大鼠血尿酸水平的影響,并從調(diào)節(jié)尿酸產(chǎn)生和排泄途徑探討其藥理機(jī)制。方法:連續(xù)給予大鼠10 g/100 mL果糖溶液8周,其中5~8周分別連續(xù)灌胃別嘌呤醇(4 mg/(kg·d))或GTP(75、150、300 mg/(kg·d)),每天1次。磷鎢酸法檢測血尿酸水平,比色法和Western blot法分別檢測肝臟黃嘌呤氧化酶(xanthine oxidase,XOD)活力及其表達(dá)水平,免疫組織化學(xué)染色法檢測腎臟中尿酸鹽轉(zhuǎn)運(yùn)子(urate transporter,UAT)、尿酸鹽陰離子轉(zhuǎn)運(yùn)子(urate-anion transporter,URAT)1和葡萄糖轉(zhuǎn)運(yùn)子(glucose transporter,GLUT)9的表達(dá)水平。結(jié)果:150、300 mg/(kg·d)GTP顯著降低了果糖誘導(dǎo)的HUA大鼠血尿酸水平(P0.05,P0.01)。同時(shí),150、300 mg/(kg·d)GTP顯著降低了HUA大鼠肝臟XOD活力和表達(dá)水平(P0.05,P0.01),顯著增強(qiáng)了腎臟UAT表達(dá)水平和降低了腎臟URAT1和GLUT9的表達(dá)水平(P0.05,P0.01)。結(jié)論:150、300 mg/(kg·d)GTP可有效降低果糖誘導(dǎo)的HUA大鼠血尿酸水平,其機(jī)制與減少尿酸產(chǎn)生和促進(jìn)尿酸排泄密切相關(guān)。
[Abstract]:Aim: to observe the effect of tea polyphenols (green tea polyphenols,GTP on serum uric acid level in rats with fructose-induced hyperuricemia (hyperuricemia,HUA) and to explore its pharmacological mechanism by regulating uric acid production and excretion pathway. Methods: rats were given 10 g 鈮，

本文編號：2485766