發(fā)布時(shí)間：2019-05-19 08:53

【摘要】：目的探討系統(tǒng)性紅斑狼瘡(SLE)患者維生素D水平與T淋巴細(xì)胞亞群及α干擾素(IFN-α)水平的相關(guān)性。方法選取SLE 80例(SLE組)分為緩解期40例(緩解期組)、活動(dòng)期40例(活動(dòng)期組),健康體檢者30例作為健康對(duì)照組。酶聯(lián)免疫吸附試驗(yàn)(ELISA)檢測(cè)外周血漿中25(OH)D、IFN-α水平;流式細(xì)胞術(shù)(FCM)測(cè)定外周血中T淋巴細(xì)胞亞群水平。結(jié)果 (1)SLE組外周血25(OH)D水平低于健康對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(P0.05);活動(dòng)期組外周血25(OH)D水平低于緩解期組,差異有統(tǒng)計(jì)學(xué)意義(P0.05);(2)SLE組外周血T淋巴細(xì)胞、Th淋巴細(xì)胞及Th/Ts與健康對(duì)照組相比顯著降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05),其中活動(dòng)期組患者低于緩解期組,差異有統(tǒng)計(jì)學(xué)意義(P0.05);SLE組與健康對(duì)照組兩組間Ts細(xì)胞計(jì)數(shù)相比差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);(3)SLE組外周血IFN-α水平高于健康對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(P0.05),活動(dòng)期組高于緩解期組,差異有統(tǒng)計(jì)學(xué)意義(P0.05);(4)SLE組外周血25(OH)D、T細(xì)胞、Th細(xì)胞計(jì)數(shù)及Th/Ts及IFN-α水平均與病情活動(dòng)度具有相關(guān)性(P0.05);(5)SLE組外周血25(OH)D與T細(xì)胞、Th細(xì)胞計(jì)數(shù)、Th/Ts計(jì)數(shù)比值呈正相關(guān),與IFN-α水平呈負(fù)相關(guān)(P0.05)。結(jié)論在SLE患者體內(nèi),維生素D水平與T淋巴細(xì)胞亞群及IFN-α水平具有顯著的相關(guān)性,推測(cè)維生素D可能通過(guò)調(diào)控T淋巴細(xì)胞亞群動(dòng)態(tài)平衡以及IFN-α水平引發(fā)機(jī)體的免疫異常。
[Abstract]:Objective to investigate the relationship between vitamin D level and T lymphocyte subsets and interferon 偽 (IFN- 偽) levels in patients with systemic lupus erythematosus (SLE). Methods 80 cases of SLE (SLE group) were divided into remission stage (n = 40), active stage (n = 40) and healthy physical examination (n = 30) as healthy control group. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of 25 (OH) D and IFN-偽 in peripheral plasma, and flow cytometry (FCM) was used to detect the level of T lymphocytes subsets in peripheral blood. Results (1) the level of 25 (OH) D in peripheral blood of SLE group was significantly lower than that of healthy control group (P 0.05), and the level of 25 (OH) D of peripheral blood of active group was lower than that of remission group (P 0.05). (2) compared with the healthy control group, the peripheral blood T lymphocytes, Th lymphocytes and Th/Ts in the SLE group were significantly lower than those in the healthy control group (P 0.05), and the patients in the active group were lower than those in the remission group. The difference was statistically significant (P 0.05). There was no significant difference in Ts cell count between SLE group and healthy control group (P 0.05). (3) the level of IFN- 偽 in peripheral blood of SLE group was significantly higher than that of healthy control group (P 0.05), and that of active group was higher than that of remission group (P 0.05). (4) in SLE group, the count of T cells, Th cells and the levels of Th/Ts and IFN- 偽 in peripheral blood 25 (OH) D were correlated with the activity of the disease (P 0.05). (5) in SLE group, 25 (OH) D of peripheral blood was positively correlated with T cell, Th cell count and Th/Ts count ratio, but negatively correlated with IFN- 偽 level (P 0.05). Conclusion Vitamin D level is significantly correlated with T lymphocyte subsets and IFN- 偽 levels in patients with SLE. It is speculated that vitamin D may induce immune abnormalities by regulating the dynamic balance of T lymphocytes subsets and the level of IFN- 偽.
【作者單位】： 新疆醫(yī)科大學(xué)第二附屬醫(yī)院風(fēng)濕免疫科;
【基金】：新疆維吾爾自治區(qū)自然科學(xué)基金資助項(xiàng)目(編號(hào):2015211C109)
【分類(lèi)號(hào)】：R593.241

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