本文選題：明膠水解物 + 酪蛋白水解物��； 參考：《中國食品學(xué)報》2017年04期

【摘要】：采用木瓜蛋白酶水解鮭魚皮明膠和酪蛋白,得到3種水解程度的明膠水解物和酪蛋白水解物,然后通過細(xì)胞活力和流式細(xì)胞術(shù)分析,分別評價水解物對成骨細(xì)胞(h FOB.19)增殖和凋亡的影響。研究結(jié)果表明,明膠水解物和酪蛋白水解物對成骨細(xì)胞均具有明確的增殖促進(jìn)作用,細(xì)胞活性分別增加103%~134%和100%~114%,水解物的水解度高,增殖促進(jìn)作用大。明膠水解物和酪蛋白水解物對依托泊苷和氟化鈉誘導(dǎo)的成骨細(xì)胞凋亡具有拮抗作用,凋亡細(xì)胞比例由31.7%下降至13.8%和22.6%(依托泊苷組),或者由19.5%下降至11.3%和17.5%(氟化鈉組)。總體看,明膠水解物對成骨細(xì)胞的增殖促進(jìn)和抗凋亡作用優(yōu)于酪蛋白水解物。
[Abstract]:Using papain to hydrolyze salmon skin gelatin and casein, three kinds of hydrolysis degree of gelatin hydrolysate and casein hydrolysate were obtained, and then analyzed by cell viability and flow cytometry. To evaluate the effects of hydrolysates on proliferation and apoptosis of osteoblasts. The results showed that both gelatin hydrolysates and casein hydrolysates had obvious effects on the proliferation of osteoblasts. The cell activity was increased by 103% and 100% respectively. The hydrolysis degree of the hydrolysates was high and the proliferation promoting effect was great. Gelatin hydrolysates and casein hydrolysates antagonized the apoptosis of osteoblasts induced by etoposide and sodium fluoride. The percentage of apoptotic cells decreased from 31.7% to 13.8% and 22.6% (etoposide group) or from 19.5% to 11.3% and 17.5% (sodium fluoride group). In general, gelatin hydrolysates have better effects on proliferation and apoptosis of osteoblasts than casein hydrolysates.
【作者單位】： 乳品科學(xué)教育部重點(diǎn)實(shí)驗室東北農(nóng)業(yè)大學(xué);東北農(nóng)業(yè)大學(xué)食品學(xué)院;
【基金】：國家自然科學(xué)基金項目(31371738) 黑龍江省對外科技合作基金項目(WB13C102)
【分類號】：R580
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本文編號：1889444