本文選題：鐵缺乏 + 生長(zhǎng)激素��； 參考：《石河子大學(xué)》2015年碩士論文

【摘要】：目的:本實(shí)驗(yàn)通過建立鐵缺乏Wistar大鼠模型,檢測(cè)大鼠鐵缺乏時(shí)血液學(xué)參數(shù)、EPO、生長(zhǎng)激素(GH)、胰島素樣生長(zhǎng)因子-1(IGF-1)水平,檢測(cè)大鼠下丘腦、垂體、腎上腺EPOR表達(dá)情況,測(cè)體重和體長(zhǎng);研究大鼠鐵缺乏時(shí)大鼠下丘腦、垂體、腎上腺組織的EPOR表達(dá)情況;鐵缺乏時(shí)大鼠血清GH、IGF-1的表達(dá)水平;初步探討大鼠鐵缺乏時(shí)對(duì)下丘腦、垂體、腎上腺EPOR和GH-IGF-1軸的影響。方法:選擇4周齡健康Wistar大鼠40只,適應(yīng)性喂養(yǎng)1周后,隨機(jī)分為兩組,每組20只,實(shí)驗(yàn)組飼以低鐵飼料,對(duì)照組飼以普通飼料。每周定期內(nèi)眥靜脈采血檢測(cè)大鼠的血液學(xué)指標(biāo),稱體重,測(cè)體長(zhǎng)。低鐵干預(yù)四周后內(nèi)眥靜脈取血檢測(cè)血清中EPO、GH和IGF-1,并取下丘腦、垂體、腎上腺組織做免疫組織化學(xué),檢測(cè)EPOR的表達(dá)情況。結(jié)果:(1)4周后實(shí)驗(yàn)組大鼠血紅蛋白(122.64±9.55 g/L)和鐵蛋白(79.77±24.99umol/L)濃度下降,兩組差異具有統(tǒng)計(jì)學(xué)意義(P0.05),造模成功。(2)兩組大鼠體重均有增加,缺鐵組增幅較小;缺鐵組大鼠血清GH、IGF-1明顯低于正常對(duì)照組,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。(3)免疫組織化學(xué)結(jié)果顯示,EPOR在對(duì)照組下丘腦中的表達(dá)較少,以0分和1分為主,高表達(dá)占12.5%;EPOR在實(shí)驗(yàn)組中表達(dá)亦較低,但1分和2分表達(dá)率均高于對(duì)照組;高表達(dá)率占37.5%,差異具有統(tǒng)計(jì)學(xué)意義。EPOR在垂體中的表達(dá)較為明顯,且實(shí)驗(yàn)組高表達(dá)率高于對(duì)照組,差異具有統(tǒng)計(jì)學(xué)意義。在觀察過程中,課題組亦發(fā)現(xiàn)EPOR在神經(jīng)垂體的表達(dá)要高于在腺垂體的表達(dá)。EPOR在腎上腺皮質(zhì)和髓質(zhì)亦均有表達(dá),且實(shí)驗(yàn)組高表達(dá)率明顯高于對(duì)照組,差異具有統(tǒng)計(jì)學(xué)意義。結(jié)論:(1)大鼠鐵缺乏時(shí)血清GH、IGF-1水平降低,抑制GH-IGF-1軸,影響其生長(zhǎng)發(fā)育。(2)大鼠鐵缺乏時(shí)下丘腦、垂體、腎上腺EPOR表達(dá)增加,以適應(yīng)鐵缺乏導(dǎo)致組織器官缺血缺氧狀態(tài),對(duì)組織器官具有保護(hù)作用。(3)大鼠鐵缺乏時(shí)EPOR對(duì)下丘腦-垂體-腎上腺軸可能起到保護(hù)作用。
[Abstract]:Objective: to establish a rat model of iron deficiency (Wistar) and to detect the levels of EPOR in hypothalamus, pituitary, adrenal gland, body weight and body length by detecting the levels of EPOR, growth hormone (GH), growth hormone (GH) and insulin-like growth factor (IGF-1) during iron deficiency in rats. To study the expression of EPOR in hypothalamus, pituitary and adrenal gland of rats with iron deficiency, and the expression level of GH IGF-1 in serum of rats with iron deficiency, and to explore the effect of iron deficiency on the axis of EPOR and GH-IGF-1 in hypothalamus, pituitary, adrenal gland and adrenal gland in rats. Methods: forty 4-week-old healthy Wistar rats were randomly divided into two groups: the experimental group was fed with low iron diet and the control group was fed with common diet. The blood samples were collected from the medial canthus vein every week to detect the hematological indexes of the rats, to weigh and to measure the body length. Serum EPO-GH and IGF-1 were collected from the inner canthus vein and the hypothalamus, pituitary and adrenal tissues were taken for immunohistochemistry to detect the expression of EPO-GH and IGF-1 after four weeks of low iron intervention. Results after 4 weeks, the concentrations of hemoglobin and ferritin (79.77 鹵24.99umoll / L) decreased in the experimental group (122.64 鹵9.55 g / L). The difference between the two groups was statistically significant (P 0.05). The results of immunohistochemistry showed that the expression of EPOR in the hypothalamus of the control group was less than that of the control group. The expression of EPOR in the experimental group was lower than that in the control group, but the expression rate of 1 and 2 points was higher than that of the control group, and the high expression rate of EPOR was significantly higher in the pituitary gland than that in the control group, and the high expression rate of EPOR in the experimental group was higher than that in the control group. The difference is statistically significant. In the course of observation, we also found that the expression of EPOR in neurohypophysis was higher than that in adenohypophysis. EPOR was also expressed in adrenal cortex and medulla, and the high expression rate of EPOR in experimental group was significantly higher than that in control group (P < 0.05). Conclusion the serum GH IGF-1 level in rats with iron deficiency decreased, the GH-IGF-1 axis was inhibited, and the growth and development of GH IGF-1 were inhibited. The expression of EPOR in hypothalamus, pituitary and adrenal gland was increased during iron deficiency in rats, in order to adapt to the ischemia and hypoxia of tissues and organs caused by iron deficiency. EPOR may protect hypothalamus-pituitary-adrenal axis during iron deficiency in rats.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R591.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 劉曉溪,李潮,李昕權(quán);缺鐵性貧血大鼠模型建立中行為體態(tài)觀察[J];中國(guó)行為醫(yī)學(xué)科學(xué);2004年03期

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本文編號(hào)：1812774