本文選題：黃芪　切入點(diǎn)：烏梅　出處：《甘肅中醫(yī)藥大學(xué)(原名：甘肅中醫(yī)學(xué)院)》2015年碩士論文

【摘要】：目的:觀察黃芪(Astragalus)烏梅(Dark Plum)配方顆粒對(duì)高脂飼料(High fat diet,Hyperlipid diet,HFD)聯(lián)合地塞米松(Dexamethasone,DEX)誘導(dǎo)的胰島素抵抗大鼠血糖水平、血脂代謝水平的影響,以及肝臟胰島素受體m RNA和骨骼肌GLUT4 m RNA表達(dá)情況,探討黃芪烏梅配方顆粒治療胰島素抵抗的作用及其機(jī)制。方法:選取雄性Wistar大鼠40只,4-6周齡,體重240±20g,隨機(jī)數(shù)表法選取35只后,高脂飼料喂養(yǎng)的同時(shí)隔日皮下注射地塞米松(1mg·kg-1)。注射結(jié)束后測(cè)定空腹血糖,規(guī)定空腹血糖升高且糖耐量異常的大鼠為造模成功大鼠。將造模后大鼠隨機(jī)分為三組:模型組、黃芪烏梅顆粒給藥組、陽(yáng)性藥物對(duì)照組(參芪降糖顆粒),另設(shè)正常對(duì)照組(n=5)。血糖試紙法檢測(cè)血糖,IPGTT法檢測(cè)葡萄糖耐量,試劑盒檢測(cè)血脂(TC、TG)、血清游離脂肪酸(FFA)、血清胰島素(FINS)水平,HE染色法觀察大鼠肝臟病理改變,RT-PCR檢測(cè)胰島素受體m RNA和GLUT4m RNA表達(dá)。結(jié)果:1、模型組空腹血糖高于正常飲食組(p0.05);葡萄糖耐量試驗(yàn)?zāi)Ｐ徒M負(fù)荷后血糖曲線(xiàn)下面積高于正常對(duì)照組(p0.05);黃芪烏梅配方顆粒治療組大鼠空腹血糖出現(xiàn)明顯下降(p0.05),胰島素抵抗指數(shù)(Homa-IR)顯著下降(p0.05)。2、模型組TC和FFA水平均明顯高于正常對(duì)照組(p0.05),黃芪烏梅配方顆粒治療后大鼠TC和FFA出現(xiàn)顯著下降(p0.05)。3、肝臟切片染色后顯示模型組的肝組織呈肝索排列紊亂及水腫樣病理改變;黃芪烏梅配方顆粒給藥組肝細(xì)胞病理改變較模型組病理改變較輕微。4、模型組大鼠肝臟組織的胰島素受體m RNA和骨骼肌組織的GLUT4 m RNA表達(dá)量低于正常組(p0.05),黃芪烏梅配方顆粒干預(yù)后大鼠胰島素受體m RNA和GLUT4m RNA表達(dá)量有所上調(diào)。結(jié)論:黃芪烏梅配方顆粒能有效地降低胰島素抵抗模型大鼠的血糖,糾正脂代謝紊亂,改善高脂血癥,其機(jī)制可能與上調(diào)肝臟組織中胰島素受體m RNA以及骨骼肌中GLUT4m RNA表達(dá)有關(guān)。
[Abstract]:Objective: to observe the effects of Astragalus Astragalus Dark Plumum (Astragalus) on blood glucose and lipid metabolism in rats with insulin resistance induced by high fat dietate (HFDs) and dexamethasone (DEX).The expression of insulin receptor m RNA in liver and GLUT4 m RNA in skeletal muscle were studied to explore the effect and mechanism of Huangqi Wumei recipe granule in the treatment of insulin resistance.Methods: 40 male Wistar rats aged 4 to 6 weeks, weight 240 鹵20g, were randomly selected and fed with high fat diet. Dexamethasone 1mg kg-1g was injected subcutaneously every other day.Fasting blood glucose (FBG) was measured at the end of the injection, and the rats with elevated fasting blood glucose and abnormal glucose tolerance were designated as successful rats.The rats were randomly divided into three groups: model group, Huangqi Wumei granule group, positive drug control group (Shenqi Jiangtang granule, normal control group).Blood glucose test paper method was used to detect glucose tolerance, serum free fatty acid (FFAA) and serum insulin (FINS) levels were measured. The expression of insulin receptor m RNA and GLUT4m RNA in rat liver was detected by reverse transcription-polymerase chain reaction (RT-PCR).Results: 1, the fasting blood glucose in the model group was higher than that in the normal diet group, the area under the blood glucose curve in the glucose tolerance test group was higher than that in the normal control group after loading, and the fasting blood glucose of the rats in the Huangqi Wumei prescription treatment group was significantly decreased (P 0.05).The insulin resistance index (Homa-IRI) decreased significantly (p0.05). The levels of TC and FFA in the model group were significantly higher than those in the normal control group (P 0.05). The TC and FFA of the model group were significantly decreased after the treatment of Huangqi Wumei recipe granule. The liver sections stained showed the liver group of the model group.Disordered arrangement of hepatic cord and edematous pathological changes were observed.Compared with the model group, the pathological changes of hepatocytes in the Huangqi Wumei prescription granule group were lighter than those in the model group. The expression of insulin receptor m RNA and GLUT4 m RNA in the liver tissue and skeletal muscle tissue of the model group were lower than that in the normal group (P 0.05), and the expression of GLUT4 m RNA in the liver tissue of the model group was lower than that in the normal group.The expression of insulin receptor m RNA and GLUT4m RNA were up-regulated after the intervention of formula granule.Conclusion: Huangqi Wumei prescription granule can effectively reduce blood sugar, correct lipid metabolism disorder and improve hyperlipidemia in rats with insulin resistance.The mechanism may be related to the up-regulation of insulin receptor m RNA in liver and GLUT4m RNA expression in skeletal muscle.
【學(xué)位授予單位】：甘肅中醫(yī)藥大學(xué)(原名：甘肅中醫(yī)學(xué)院)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：R587.1

【參考文獻(xiàn)】

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本文編號(hào)：1721290