發(fā)布時間：2018-03-16 12:47

  本文選題：骨髓　切入點：間充質(zhì)干細胞　出處：《中國職業(yè)醫(yī)學(xué)》2017年05期 　論文類型：期刊論文

【摘要】：目的探討骨髓間充質(zhì)干細胞(BMSCs)對苯中毒小鼠造血損傷的修復(fù)作用。方法取無特定病原體級健康雄性昆明小鼠5只,以全骨髓貼壁法分離培養(yǎng)BMSCs。取同類小鼠,以苯油混合物進行多點皮下注射染毒(連續(xù)10周,每周3次),建立苯中毒動物模型后,隨機分為苯中毒組和BMSCs移植組,每組18只,分別經(jīng)尾靜脈輸注250.0μL的0.9%氯化鈉溶液或細胞密度為2×10~9/L的BMSCs,每周1次,連續(xù)治療4周。另設(shè)對照組小鼠(10只),不予任何處理。治療結(jié)束2周后處死各組小鼠,檢測血常規(guī),以瑞氏-吉姆薩法染色觀察小鼠骨髓有核細胞,計數(shù)骨髓造血祖細胞集落數(shù)量,以酶聯(lián)免疫吸附實驗檢測小鼠血清中γ-干擾素(IFN-γ)水平。結(jié)果成功制備苯中毒動物模型。BMSCs移植療程結(jié)束后,苯中毒組小鼠周圍血中白細胞計數(shù)、血小板計數(shù)、紅細胞計數(shù)、血紅蛋白水平和骨髓中有核細胞總數(shù)、粒細胞-巨噬細胞集落形成單位(CFU-GM)均低于對照組(P0.01),BMSCs治療組小鼠上述指標均高于苯中毒組(P0.05);BMSCs治療組小鼠血小板計數(shù)、紅細胞計數(shù)和骨髓中有核細胞總數(shù)、CFU-GM均低于對照組(P0.05)。苯中毒組小鼠血清中IFN-γ水平高于對照組(P0.01);BMSCs治療組小鼠該指標低于苯中毒組(P0.01),但與對照組比較,差異無統(tǒng)計學(xué)意義(P0.05)。結(jié)論 BMSCs對苯所致的造血系統(tǒng)損傷具有修復(fù)作用。
[Abstract]:Objective to investigate the repair effect of bone marrow mesenchymal stem cells (BMSCs) on hematopoietic injury in benzene intoxicated mice. Methods five healthy male Kunming mice without specific pathogens were isolated and cultured by whole bone marrow adherent method. The animal model of benzene poisoning was established by subcutaneous injection of benzene oil mixture (10 consecutive weeks, 3 times a week). The animal model was randomly divided into benzene poisoning group and BMSCs transplantation group, 18 rats in each group. 0.9% sodium chloride solution of 250.0 渭 L or BMSCs with cell density of 2 脳 10 ~ (9) / L were injected via tail vein once a week for 4 weeks. 10 mice in the control group were treated without any treatment. After 2 weeks of treatment, the mice in each group were sacrificed and their blood routine was detected. The nucleated bone marrow cells of mice were observed by Rish-Jim 's method and the number of hematopoietic progenitor cells in bone marrow was counted. The level of IFN- 緯 in serum of mice was detected by enzyme-linked immunosorbent assay (Elisa). Results the animal model of benzene poisoning was established successfully. After the course of transplantation of BMSCs, the peripheral white blood cell count, platelet count and erythrocyte count in benzene poisoning group were measured. The levels of hemoglobin, the total number of nucleated cells in bone marrow, and the CFU-GM) of granulocyte-macrophage colony forming unit (CFU-GM) were all lower than those of the control group (P 0.01) and the above indexes were higher than those of the group treated with benzene poisoning (P 0.05) and the platelet count of the group treated with BMSCs were higher than those of the group treated with benzene poisoning. The count of erythrocyte and the total number of nucleated cells in bone marrow were lower than that of control group (P 0.05). The level of IFN- 緯 in serum of mice in benzene poisoning group was higher than that in control group (P 0.01), but the level of IFN- 緯 in BMSCs group was lower than that in control group (P 0.01), but it was higher than that in control group. Conclusion BMSCs can repair the damage of hematopoietic system induced by benzene.
【作者單位】： 廣東省職業(yè)病防治院廣東省職業(yè)病防治重點實驗室;成都軍區(qū)昆明總醫(yī)院干細胞與免疫細胞生物技術(shù)國家地方聯(lián)合工程實驗室;
【基金】：國家科技支撐計劃項目(2014BAI12B01) 國家臨床重點�？平ㄔO(shè)項目(2011-09) 廣東省職業(yè)病防治重點實驗室(2012A061400007) 廣東省科技計劃項目(2014A020212551,2014A020212500,2016A020215131) 廣東省醫(yī)學(xué)科研基金(A2015624,A2016155,A2016458) 云南省衛(wèi)生科技計劃項目(2014NS132)
【分類號】：R135.1

【相似文獻】

相關(guān)期刊論文 前5條

1 ;臍帶,送給寶寶的一份健康大禮(五)——“臍帶間充質(zhì)干細胞”產(chǎn)業(yè)化獲得成功[J];健康人生;2007年05期

2 ;臍帶,送給寶寶的一份健康大禮(六)——“臍帶間充質(zhì)干細胞”產(chǎn)業(yè)化獲得成功[J];健康人生;2007年06期

3 楊銀書;羅芳;趙紅斌;王劍鋒;王先華;周煥發(fā);李強;;多氯聯(lián)苯對大鼠間充質(zhì)干細胞c-myc和-βcatenin表達的影響[J];解放軍預(yù)防醫(yī)學(xué)雜志;2012年02期

4 付岳;王彥;杜利清;徐暢;曹嘉;陳鳳華;郭艷婷;劉建香;劉強;樊飛躍;蘇旭;;Sirt1在輻射誘導(dǎo)的IL-1β表達中的作用[J];輻射研究與輻射工藝學(xué)報;2013年05期

5 李偉;;臍帶,送給寶寶的一份健康大禮(三)[J];健康人生;2007年03期

相關(guān)重要報紙文章 前1條

1 陳超;青春期減肥過度會致骨質(zhì)疏松[N];中國中醫(yī)藥報;2006年

，

本文編號：1619979