本文選題：心鈉素　切入點：哮喘　出處：《重慶醫(yī)學》2017年22期 　論文類型：期刊論文

【摘要】：目的探討外源性心鈉素對過敏性哮喘小鼠模型氣道炎癥的影響作用及其作用機制。方法選取SPF級BALBc小鼠48只,采用隨機數字表法分為對照組(等量生理鹽水干預)、模型組(等量生理鹽水干預)、實驗組A(外源性心鈉素0.5μg/g)、實驗組B(外源性心鈉素0.5μg/g+A71915)。模型組、實驗組A、實驗組B建模成功后給予對應處理措施,在最后一次激發(fā)實驗后24h,采集各組血清、支氣管肺泡灌洗液檢查炎癥因子,采用HE染色觀察肺組織病理變化,采用Western blot技術檢測肺組織中鋅指轉錄因子(GATA3)蛋白的表達水平。結果模型組小鼠的血液中EOS、淋巴細胞、中性粒細胞所占比例均高于對照組(P0.05),實驗組A的血液中EOS、淋巴細胞、中性粒細胞所占比例均高于模型組、實驗組B(P0.05);模型組小鼠的肺泡灌洗液中EOS、淋巴細胞、中性粒細胞計數、IL-6、TNF-α水平均高于對照組(P0.05),實驗組A的肺泡灌洗液中EOS、淋巴細胞、中性粒細胞計數、IL-6、TNF-α水平均高于模型組、實驗組B(P0.05);模型組小鼠的肺組織中GATA3蛋白表達水平均高于對照組(P0.05),實驗組A肺組織中GATA3蛋白表達水平高于模型組、實驗組B(P0.05)。結論外源性心鈉素會進一步加劇過敏性哮喘小鼠模型的氣道炎癥反應,與激活炎性細胞、炎癥因子釋放及增強肺組織中GATA3蛋白表達有關。
[Abstract]:Objective to investigate the effect and mechanism of exogenous atrial natriuretic peptide (ANP) on airway inflammation in allergic asthmatic mice. Methods 48 SPF grade BALBc mice were selected. The rats were randomly divided into control group, model group, experimental group A (external atrial natriuretic peptide 0.5 渭 g / g) and experimental group B (external atrial natriuretic peptide 0.5 渭 g / g A71915). Experimental group A and group B were given corresponding treatment after successful modeling. 24 hours after the last exciting experiment, the serum and bronchoalveolar lavage fluid of each group were collected to examine the inflammatory factors, and the pathological changes of lung tissue were observed by HE staining. Western blot technique was used to detect the expression of zinc finger transcription factor (GATA3) protein in lung tissue. Results the percentage of EOS, lymphocytes and neutrophils in the blood of the model group was higher than that in the control group (P 0.05), and that in the blood of the experimental group A was higher than that of the control group. The percentage of neutrophils in the model group was higher than that in the model group, and the levels of EOS, lymphocytes and neutrophils in the alveolar lavage fluid of the model group were higher than those in the control group, and the levels of EOS and lymphocytes in the alveolar lavage fluid of the experimental group were higher than those in the control group. The level of IL-6 TNF- 偽 in the model group was higher than that in the model group, and the expression level of GATA3 protein in the lung tissue of the model group was higher than that of the control group (P 0.05), and the expression level of GATA3 protein in the lung tissue of the experimental group was higher than that of the model group. Conclusion exogenous atrial natriuretic peptide can further aggravate the airway inflammation in allergic asthma mice, which is related to the activation of inflammatory cells, the release of inflammatory factors and the enhancement of GATA3 protein expression in lung tissue.
【作者單位】： 天津市第五中心醫(yī)院呼吸內科;
【基金】：天津市衛(wèi)生局資助基金項目(tj20150823)
【分類號】：R562.25

【相似文獻】

相關期刊論文 前10條

1 孟泳;齊景憲;;心鈉素與肺部疾病研究進展[J];中國誤診學雜志;2007年24期

2 歐陽偉;;心鈉素在哮喘中的擴支氣管作用[J];國外醫(yī)學(內科學分冊);1990年11期

3 王業(yè)亞,胡克,趙俊;阻塞性睡眠呼吸暫停綜合征患者夜間多尿與腦鈉素和心鈉素的關系[J];中國臨床康復;2005年11期

4 王旭兒,陸航;肺炎患兒血漿內皮素和心鈉素及動脈血氧分壓間關系初探[J];中國實用兒科雜志;1998年01期

5 林松娟,趙學蘭,趙瑞蓮;阻塞性睡眠呼吸暫停綜合征患者腎功能和心鈉素的變化分析[J];濰坊醫(yī)學院學報;2003年05期

6 肖永龍,殷凱生,鄭培德,戴令娟,杭美琴,朱世均,侯杰;睡眠呼吸暫停綜合征患者尿尿酸、心鈉素、腎素醛固酮的變化及意義[J];中華結核和呼吸雜志;2000年01期

7 趙慶蓉;慢性阻塞性肺疾病急性期血漿內皮素和心鈉素測定及臨床意義[J];川北醫(yī)學院學報;1997年03期

8 馮長順;劉增軍;楊建軍;曾強;黃念秋;;老年慢性喘息型支氣管炎血漿內皮素-1和心鈉素水平動態(tài)觀察[J];解放軍保健醫(yī)學雜志;1999年03期

9 張秋美,張心中,王曉欣;心鈉素、癌胚抗原及鐵蛋白聯合檢測的臨床意義[J];醫(yī)學綜述;2003年01期

10 譚玉萍;黃美杏;;心鈉素含量在胸腔積液性質鑒別中的應用綜述[J];廣西中醫(yī)學院學報;2006年02期

相關會議論文 前1條

1 張心中;張英凱;馬金衛(wèi);李師彬;朱春黎;;心鈉素、癌胚抗原及鐵蛋白聯合測定對良、惡性胸腔積液的鑒別價值[A];科技、工程與經濟社會協調發(fā)展——中國科協第五屆青年學術年會論文集[C];2004年

相關碩士學位論文 前1條

1 喬珊;睡眠呼吸暫停綜合征患者心鈉素變化及意義[D];昆明醫(yī)學院;2003年

，

本文編號：1604958