本文選題：核電磁脈沖　切入點：累積輻照　出處：《華東師范大學》2016年碩士論文　論文類型：學位論文

【摘要】：電磁暴露的人體安全性是一個日益受到關注的問題,并由此展開了大量的研究工作。隨著研究的發(fā)展,人們發(fā)現(xiàn)電磁脈沖(EMP)暴露引起的非熱效應不容忽視。核電磁脈沖(NEMP)是電磁脈沖的一種。在前期研究中發(fā)現(xiàn),以細胞增殖作為考查指標時,NEMP單次暴露量與即時生物效應之間存在著一定的劑量學關系。那么,生物活體是否具有類似的量效關系?NEMP單次輻照所產(chǎn)生的即時效應是否會恢復?在單次輻照不產(chǎn)生即時效應的情況下,周期性多次輻照是否會產(chǎn)生累積生物效應?為了回答這些問題,本文以大鼠肝臟和心臟組織作為研究對象,圍繞著上述問題展開了動物水平上的研究,以期為NEMP暴露的安全標準的制定提供更全面的實驗依據(jù)。本文首先研究了不同場強(E分別為110kV/m、140kV/m、297 kV/m和873kV/m)和不同脈沖個數(shù)(N分別為2、5和100個)的NEMP單次輻照對雄性大鼠肝臟及心臟組織細胞凋亡的影響,以及隨照后時間延長的自我修復情況。其中,采用全自動血液分析儀和生化分析儀檢測了大鼠血常規(guī)指標和血液中與肝臟和心臟功能相關的血清生化指標(谷丙轉氨酶ALT、谷草轉氨酶AST)的含量；通過末端轉移酶標記(Tunel)法標記組織凋亡細胞,從組織形態(tài)上觀察了肝臟與心臟細胞的凋亡情況；通過酶聯(lián)免疫吸附試驗(Elisa)法檢測了肝臟和心臟組織細胞凋亡相關蛋白(促凋亡蛋白Bax、抑凋亡蛋白Bcl-2)在總蛋白TP中的含量水平。實驗檢測了上述各指標在單次輻照后6h、1d、3d、7d、10d、15d的變化情況,以獲得細胞損傷與恢復的相關信息。實驗結果表明：隨著輻照劑量的增加,大鼠肝臟和心臟的受損程度增加,在低場強、脈沖個數(shù)很少(E=110 kV/m,N=2)時,無明顯的即時效應；當場強較低(E=110 kV/m、140 kV/m, N=5、100)時,有即時效應且在1 d后恢復或基本恢復；當場強較高(E=297kV/m、873 kV/m,N=5、100)時,有明顯的即時效應且修復困難。本文隨后選取了無明顯即時效應(E=110 kV/m, N=2)、有即時效應但已恢復(E=110 kV/m、N=5、100)、有即時效應但未恢復(E=297 kV/m, N=5、100)條件下多次累積輻照產(chǎn)生的組織損傷情況。周期性累積輻照時間分別為7d、14d、21 d和28d,輻照時間間隔為24h。檢測指標與方法不變。實驗結果表明：NEMP周期性暴露對大鼠肝臟和心臟組織細胞有累積損傷作用,其累積效應的總效果與單次輻照的即時損傷程度以及累積輻照天數(shù)均有關聯(lián),在本實驗條件下可以略粗地用線性關系來表示。
[Abstract]:The human safety of electromagnetic exposure is an issue of increasing concern, and a lot of research work has been carried out. With the development of research, It has been found that the non-thermal effect caused by EMP) exposure can not be ignored. Nuclear electromagnetic pulse NEMPis a kind of electromagnetic pulse. There is a dosimetric relationship between the single exposure of NEMP and the immediate biological effect when cell proliferation is used as an index. So is there a similar dose-effect relationship in living organisms? Will the immediate effect of single NEMP irradiation recover? If a single irradiation does not produce an immediate effect, will periodic multiple exposures produce cumulative biological effects? In order to answer these questions, we studied the liver and heart tissues of rats at the animal level. In order to provide a more comprehensive experimental basis for the formulation of safety standards for NEMP exposure, this paper first studied the effects of single irradiation of NEMP with different field intensities (E = 110 kV / m ~ (-1) = 140 kV / m ~ (97) and 873 kV / m ~ (-1) and N = 2 ~ 5 and N ~ (100) respectively) on male size. The effect of apoptosis in liver and heart of mice, And self-healing over time. The blood routine indexes and serum biochemical indexes related to liver and heart function in rats were measured by automatic blood analyzer and biochemical analyzer. The apoptotic cells of liver and heart were observed by terminal transferase labeling with Tunel method. Enzyme linked immunosorbent assay (Elisa) was used to detect the content of apoptosis-related protein (apoptosis-promoting protein Bax-2) in liver and heart tissues in total protein TP. After 6 hours, the change of 1d ~ 3d ~ 7d ~ 7d ~ (10) d ~ (10) ~ (th) ~ (15) d, The results showed that the damage degree of liver and heart increased with the increase of irradiation dose, and there was no obvious immediate effect at low field intensity and few pulses. There was an immediate effect on the spot and recovered or basically recovered after 1 day, while the higher one was 297kV / mr-1 (873 KV / mNV / MN / N5 / 100) on the spot. There is obvious immediate effect and repair difficulty. Subsequently, we select the tissue damage caused by multiple cumulative irradiation under the condition of no obvious immediate effect (110 KV / mHN = 2), which has immediate effect but has recovered 110kV / mHN 5100, and which has immediate effect but does not recover EO297kV / mHN ~ (5100) (n = 10). The results of this paper are as follows: (1) in this paper, we select the tissue damage caused by multiple accumulative irradiation under the condition of no obvious immediate effect and no obvious real time effect. The results showed that the accumulative irradiation time was 7 days, 14 days and 28 days, respectively, and the interval of irradiation time was 24 hours. The detection indexes and methods were not changed. The experimental results showed that the periodic exposure of 1: NEMP had cumulative damage effect on rat liver and heart tissue cells. The total effect of cumulative effect is correlated with the degree of immediate damage and the number of days of cumulative irradiation, which can be expressed by linear relation in this experiment.
【學位授予單位】：華東師范大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R594.8

【相似文獻】

相關會議論文 前2條

1 張繼坤;李傳應;;濾波技術在核電磁脈沖防護中的應用研究[A];第十三屆全國核電子學與核探測技術學術年會論文集（上冊）[C];2006年

2 朱偉玲;胡皓全;趙家升;楊顯清;;核電磁脈沖對傳輸線耦合的研究[A];第17屆全國電磁兼容學術會議論文集[C];2007年

相關碩士學位論文 前6條

1 林錦文;核電磁脈沖輻照對大鼠肝臟和心臟組織細胞凋亡的影響[D];華東師范大學;2016年

2 余紹斌;核電磁脈沖及其工程防護[D];西安電子科技大學;2008年

3 丁昱;核電磁脈沖線纜耦合效應研究[D];北京交通大學;2009年

4 呂永亮;屏蔽設施的核電磁脈沖綜合防護研究[D];西安電子科技大學;2013年

5 歷園園;高空核電磁脈沖輻射下的艦船電磁特性研究[D];哈爾濱工程大學;2012年

6 舒志強;核電磁脈沖孔耦合及防護研究[D];西安電子科技大學;2009年

，

本文編號：1592173