本文選題：糖尿病　切入點：脂肪酸　出處：《中國藥理學通報》2017年02期 　論文類型：期刊論文

【摘要】：目的糖尿病上調(diào)肝臟CYP1A2的功能與表達,但機制尚未明確。本研究擬從脂肪酸角度,通過體外研究初步探索糖尿病上調(diào)肝臟CYP1A2功能與表達的作用機制。方法通過測定CYP1A2底物非那西丁代謝水平考察肝細胞HepG2和Fa2N-4細胞上CYP1A2的酶活性,通過熒光實時定量PCR方法檢測細胞上CYP1A2 mRNA表達水平。分別用糖尿病大鼠(Ⅰ型和Ⅱ型)和正常大鼠血清培養(yǎng)HepG2細胞48 h,考察細胞上CYP1A2的功能。在培養(yǎng)基中加入不同濃度的飽和脂肪酸(軟脂酸和硬脂酸)和不飽和脂肪酸(油酸和亞油酸)培養(yǎng)HepG2和Fa2N-4細胞48 h,測定細胞上CYP1A2的功能與表達。結(jié)果Ⅰ型和Ⅱ型糖尿病大鼠血清培養(yǎng)的HepG2細胞上CYP1A2酶活性明顯增高。高濃度的脂肪酸均可增加2種細胞上CYP1A2功能與表達。結(jié)論糖尿病狀態(tài)下脂肪酸濃度的異常變化可能是影響肝臟CYP1A2功能與表達上調(diào)的原因之一,本研究為進一步探索糖尿病狀態(tài)下肝臟CYP1A2改變機制提供了一定的基礎(chǔ)。
[Abstract]:Objective Diabetes mellitus can up-regulate the function and expression of CYP1A2 in liver, but the mechanism is not clear. Methods the enzyme activity of CYP1A2 on HepG2 and Fa2N-4 cells was determined by measuring the metabolic level of CYP1A2 substrate phenacetin. The expression of CYP1A2 mRNA was detected by real-time quantitative PCR. The HepG2 cells were cultured in the serum of diabetic rats (type 鈪，

本文編號：1566598