本文關鍵詞： 多發(fā)性硬化 臍帶間充質(zhì)干細胞 鞘氨醇激酶 實驗性自身免疫性腦脊髓炎 小鼠　出處：《鄭州大學學報(醫(yī)學版)》2017年02期 　論文類型：期刊論文

【摘要】：目的:觀察鞘氨醇激酶1(SPK1)基因轉(zhuǎn)染的臍帶間充質(zhì)干細胞(UCMSC)移植對實驗性自身免疫性腦脊髓炎(EAE)小鼠的療效。方法:將C57BL/6小鼠分為對照組(12只)、EAE組、UCMSC組和UCMSC-SPK1組;后3組進行免疫誘導以制備EAE模型,每組11只。以第一次注射抗原當日為第0天,UCMSC組和UCMSC-SPK1組小鼠分別在免疫后第14、21、28天通過尾靜脈注射UCMSC和UCMSC-SPK1,對照組和EAE組注射同等體積的生理鹽水。每日采用雙盲法進行小鼠神經(jīng)功能缺損評分;第33天,ELISA法檢測外周血IFN-γ、TNF-α、IL-17和L-4水平,分別采用HE和Fast-blue(LFB)染色觀察并進行脊髓炎性細胞浸潤評分、脫髓鞘評分,免疫組化法檢測脊髓GFAP和MBP的表達,流式細胞術檢測脾臟中調(diào)節(jié)性T細胞和NK細胞比例。結(jié)果:與EAE組比較,UCMSC-SPK1組和UCMSC組小鼠神經(jīng)功能缺損評分、脊髓炎性細胞浸潤評分和脫髓鞘評分降低,脾臟調(diào)節(jié)性T細胞比例升高、NK細胞比例降低;UCMSC-SPK1組變化更為顯著(P0.05);UCMSC-SPK1組脊髓GFAP表達較EAE組下降,MBP表達增強(P0.05),外周血IFN-γ、TNF-α、IL-17水平降低,IL-4水平升高(P0.05)。結(jié)論:UCMSC-SPK1移植能較UCMSC更為顯著地修復EAE小鼠神經(jīng)功能損傷。
[Abstract]:Objective: to observe the effect of UCM SCC transplantation on experimental autoimmune encephalomyelitis (EAE) with sphingosine kinase 1 (SPK1) gene transfected umbilical cord mesenchymal stem cells (UCM SCC). Methods: C57BL / 6 mice were divided into control group (n = 12). EAE group, UCMSC group and UCMSC-SPK1 group; The last three groups were immunized to make EAE model with 11 mice in each group. The mice in UCMSC group and UCMSC-SPK1 group were immunized at the 14th day after immunization. UCMSC and UCMSC-SPK1 were injected through tail vein for 28 days, and the same volume of normal saline was injected into control group and EAE group. The levels of IL-17 and L-4 in peripheral blood of IFN- 緯 -TNF- 偽 were detected by Elisa on the 33rd day. The expression of GFAP and MBP in spinal cord were detected by HE and Fast-blueblueLFB staining respectively. The spinal cord inflammatory cell infiltration score demyelination score and immunohistochemical method were used to detect the expression of GFAP and MBP in spinal cord. Results: compared with EAE group, UCMSC-SPK1 group and UCMSC group were compared with UCMSC-SPK1 group and UCMSC group. The scores of inflammatory cell infiltration and demyelination of the spinal cord decreased, while the proportion of regulatory T cells in the spleen increased and the proportion of NK cells decreased. The changes in UCMSC-SPK1 group were more significant than those in P0.05 group. The expression of GFAP in spinal cord in UCMSC-SPK1 group was higher than that in EAE group, and the level of IL-17 in peripheral blood was lower than that in EAE group. Conclusion the transplantation of IL-4 can repair the nerve function injury of EAE mice more significantly than that of UCMSC. Conclusion\% UCMSC-SPK1 transplantation is more effective than UCMSC in repairing nerve function injury in EAE mice.
【作者單位】： 鄭州大學第二附屬醫(yī)院神經(jīng)內(nèi)科;中國人民解放軍第148醫(yī)院神經(jīng)內(nèi)科;首都醫(yī)科大學三博腦科醫(yī)院神經(jīng)內(nèi)科;海軍總醫(yī)院海戰(zhàn)傷研究所;軍事醫(yī)學科學院放射與輻射醫(yī)學研究所;
【基金】：國家自然科學基金青年基金項目81201760
【分類號】：R744.51
【正文快照】： 1)鄭州大學第二附屬醫(yī)院神經(jīng)內(nèi)科鄭州450014 2)中國人民解放軍第148醫(yī)院神經(jīng)內(nèi)科山東淄博255300 3)首都醫(yī)科大學三博腦科醫(yī)院神經(jīng)內(nèi)科北京100093 4)海軍總醫(yī)院海戰(zhàn)傷研究所北京100037 5)軍事醫(yī)學科學院放射與輻射醫(yī)學研究所北京100850#通信作者:王運良,男,1964年11月生,博士,

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