目的探討內(nèi)皮型一氧化氮合酶（endothelial nitric oxide synthase,e NOS）和一氧化氮（nitric oxide,NO）在單側(cè)輸尿管梗阻（unilateral ureteral obstruction,UUO）腎間質(zhì)纖維化小鼠微血管病變中的作用及機(jī)制。方法64只KM小鼠隨機(jī)分為兩組:假手術(shù)組n=32只;單側(cè)輸尿管梗阻UUO組n=32只。觀察4周,每周檢測各組小鼠血BUN、Scr及一氧化氮,流式細(xì)胞計(jì)數(shù)外周血CD133+/VEGFR+內(nèi)皮祖細(xì)胞（endothelial progenitor cells,EPCs）、Masson染色觀察腎組織形態(tài)學(xué)變化,免疫組化法檢測腎間質(zhì)CD34+表達(dá)計(jì)數(shù)微血管密度,實(shí)時(shí)定量PCR檢測腎皮質(zhì)e NOS、VEGF mRNA表達(dá)。結(jié)果 UUO組血一氧化氮、內(nèi)皮祖細(xì)胞計(jì)數(shù)、腎間質(zhì)微血管密度、e NOS、VEGF mRNA表達(dá)水平持續(xù)下降,在第2、3、4周與對照組差異有統(tǒng)計(jì)學(xué)意義。一氧化氮水平與腎間質(zhì)微血管密度呈正相關(guān)（r=0.715,P<0.05）;e NOS mRNA表達(dá)水平與腎間質(zhì)微血管密度（r=0.624,P<... 

【文章來源】：中國實(shí)驗(yàn)動(dòng)物學(xué)報(bào). 2015,23(05)北大核心CSCD

【文章頁數(shù)】：6 頁

【部分圖文】：

UUO組小鼠各時(shí)間點(diǎn)流式細(xì)胞圖

中國實(shí)驗(yàn)動(dòng)物學(xué)報(bào)2015年10月第23卷第5期ActaLabAnimSciSin，October2015，Vol．23No．5注:1A:UUO組(1周);1B:UUO組(2周);1C:UUO組(3周);1D:UUO組(4周)。圖1UUO組小鼠各時(shí)間點(diǎn)流式細(xì)胞圖Note．1A:UUOgroup(1week);1B:UUOgroup(2weeks);1C:UUOgroup(3weeks);1D:UUOgroup(4weeks)．Fig．1CD133+/VEGFＲ+endothelialprogenitorcellsinperipheralbloodmononuclearcellsoftheUUOgroupatdifferenttimes注:A:Sham組(1周);B:Sham組(2周);C:Sham組(3周);D:Sham組(4周)。圖2Sham組小鼠各時(shí)間點(diǎn)流式細(xì)胞圖Note．A:Shamgroup，1week;B:Shamgroup，2weeks;C:Shamgroup，3weeks;D:Shamgroup，4weeks．Fig．2CD133+/VEGFＲ+endothelialprogenitorcellsinperipheralbloodmononuclearcellsoftheshamgroupatdifferenttimes注:A:Sham組;B:UUO組(1周);C:UUO組(2周);D:UUO組(3周);E:UUO組(4周)。圖3兩組小鼠腎臟病理(Masson，×200HPF)Note．A:Shamgroup;B:UUOgroup(1week);C:UUOgroup(2weeks);D:UUOgroup(3weeks);E:UUOgroup(4weeks)．Fig．3Morphologicalchangesintherenaltissueunderopticalmicroscope(Massonstaining，×200HPF)487

中國實(shí)驗(yàn)動(dòng)物學(xué)報(bào)2015年10月第23卷第5期ActaLabAnimSciSin，October2015，Vol．23No．5注:1A:UUO組(1周);1B:UUO組(2周);1C:UUO組(3周);1D:UUO組(4周)。圖1UUO組小鼠各時(shí)間點(diǎn)流式細(xì)胞圖Note．1A:UUOgroup(1week);1B:UUOgroup(2weeks);1C:UUOgroup(3weeks);1D:UUOgroup(4weeks)．Fig．1CD133+/VEGFＲ+endothelialprogenitorcellsinperipheralbloodmononuclearcellsoftheUUOgroupatdifferenttimes注:A:Sham組(1周);B:Sham組(2周);C:Sham組(3周);D:Sham組(4周)。圖2Sham組小鼠各時(shí)間點(diǎn)流式細(xì)胞圖Note．A:Shamgroup，1week;B:Shamgroup，2weeks;C:Shamgroup，3weeks;D:Shamgroup，4weeks．Fig．2CD133+/VEGFＲ+endothelialprogenitorcellsinperipheralbloodmononuclearcellsoftheshamgroupatdifferenttimes注:A:Sham組;B:UUO組(1周);C:UUO組(2周);D:UUO組(3周);E:UUO組(4周)。圖3兩組小鼠腎臟病理(Masson，×200HPF)Note．A:Shamgroup;B:UUOgroup(1week);C:UUOgroup(2weeks);D:UUOgroup(3weeks);E:UUOgroup(4weeks)．Fig．3Morphologicalchangesintherenaltissueunderopticalmicroscope(Massonstaining，×200HPF)487

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期刊論文
[1]阿霉素腎病小鼠的腎臟病理轉(zhuǎn)變過程[J]. 劉練,張高福,李秋,王墨.  中國實(shí)驗(yàn)動(dòng)物學(xué)報(bào). 2014(02)



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