【摘要】：目的:觀察缺血后處理對大鼠肢體缺血再灌注(LIR)后腎組織細胞凋亡的影響,探討其可能機制。方法:30只SD大鼠隨機分為對照組、缺血再灌注組(IR組)和缺血后處理加再灌注組(I-postC組),每組10只。建立大鼠肢體缺血再灌注(LIR)模型,即橡皮帶環(huán)繞大鼠雙后肢根部阻斷血流4h再恢復血流灌注4h。對照組大鼠僅松弛環(huán)繞橡皮帶不阻斷血流,I-postC組大鼠則在再灌注前附加反復3次缺血5min-再灌注5min操作,即缺血后處理。全自動生化分析儀測各組大鼠血漿肌酐(Cr)、尿素氮(BUN)和C反應蛋白(CRP)水平;免疫組織化學法檢測大鼠腎組織凋亡相關蛋白Bcl-2和Bax的表達,采用自動圖像分析系統(tǒng)統(tǒng)計其定量結(jié)果并計算Bcl-2/Bax比值;TUNEL染色后在激光共聚焦顯微鏡下觀察腎組織細胞凋亡情況,電鏡下觀察腎組織超微結(jié)構。結(jié)果:與對照組比較,IR組和I-postC組大鼠血漿Cr、BUN和CRP水平均明顯增高(P0.01);與IR組比較,I-postC組大鼠血漿Cr、BUN和CRP水平均明顯降低(P0.01)。與對照組比較,IR組和I-postC組大鼠腎組織中Bax和Bcl-2表達水平明顯升高(P0.05或P0.01),Bcl-2/Bax比值降低(P0.05);與IR組比較,I-postC組大鼠腎組織中Bax表達水平降低(P0.05),Bcl-2表達水平升高(P0.01),Bcl-2/Bax比值升高(P0.05)。激光共聚焦顯微鏡下觀察,與對照組比較,IR組大鼠腎組織中凋亡細胞明顯增多;與IR組比較,I-postC組大鼠腎組織中凋亡細胞明顯減少。透射電鏡下觀察,對照組大鼠腎組織細胞結(jié)構清晰完整;IR組大鼠腎組織中腎近曲小管上皮細胞核固縮,溶酶體和致密顆粒沉積增多,線粒體數(shù)目減少,部分線粒體嵴斷裂或模糊,腎小球足突細胞突起不規(guī)則、融合,有空泡現(xiàn)象,粗面內(nèi)質(zhì)網(wǎng)擴張;與IR組比較,I-postC組大鼠腎組織中腎小管上皮細胞及腎小球損傷有一定程度改善。結(jié)論:LIR可誘發(fā)大鼠腎組織細胞凋亡,缺血后處理可抑制肢體缺血再灌注后的腎細胞凋亡,對改善大鼠腎功能有一定作用。
[Abstract]:Aim: to observe the effect of post-ischemic treatment on apoptosis of renal tissue after limb ischemia reperfusion (LIR) in rats and to explore its possible mechanism. Methods Thirty Sprague-Dawley rats were randomly divided into control group, ischemia reperfusion group (IR group) and post-ischemic plus reperfusion group (I-postC group) with 10 rats in each group. The (LIR) model of limb ischemia-reperfusion was established in rats, that is, the rubber band around the roots of the hind limbs blocked the blood flow for 4 h and then recovered the blood flow for 4 h. In the control group, the rats in the I-postC group were treated with repeated 5 min-reperfusion 5min for 3 times before reperfusion, that is, after ischemia. The levels of plasma creatinine (Cr), urea nitrogen (BUN) and C-reactive protein (CRP) were measured by automatic biochemical analyzer and the expression of apoptosis-related proteins Bcl-2 and Bax in renal tissue of rats were detected by immunohistochemical method. The quantitative results were analyzed by automatic image analysis system and the Bcl-2/Bax ratio was calculated by Tunel staining. The apoptosis of renal tissue was observed under laser confocal microscope and the ultrastructure of renal tissue was observed under electron microscope. Results: compared with the control group, the plasma levels of Cr bun and CRP in IR group and I-postC group were significantly increased (P0.01), and those in I-postC group were significantly lower than those in IR group (P0.01). Compared with the control group, the expression of Bax and Bcl-2 in renal tissue of IR group and I-postC group were significantly increased (P0.05 or P0.01) and the ratio of Bcl-2 / Bax was decreased (P0.05), and the expression of Bax in renal tissue of I-postC group was lower than that of IR group (P0.05). The expression level of Bcl-2 was increased (P0.01) and the ratio of Bcl-2 / Bax was increased (P0.05). Compared with the control group, the apoptotic cells in renal tissue of IR group were significantly increased, and those in I-postC group were significantly decreased compared with IR group under confocal laser microscope. Under transmission electron microscope, the renal cell structure of the control group was clear and intact. In IR group, the renal proximal convoluted tubule epithelium was pyknosis, lysosome and dense granules were increased, and the number of mitochondria was decreased. Some mitochondria cristae were broken or blurred, glomerular podocytes were irregular, fused, empty vesicles, rough endoplasmic reticulum dilated, and renal tubular epithelial cells and glomerular damage in I-postC group were improved to some extent compared with IR group. Conclusion: WLIR can induce renal cell apoptosis in rats, and post ischemic treatment can inhibit the apoptosis of renal cells after limb ischemia reperfusion, which can improve the renal function of rats to some extent.
【作者單位】： 華北理工大學基礎醫(yī)學院病理生理學系;
【基金】：河北省衛(wèi)計委醫(yī)學研究重點項目資助課題(20130060)
【分類號】：R692

【參考文獻】

相關期刊論文 前3條

1 趙利軍;李開濟;盧秋玲;門秀麗;;缺血后處理對大鼠肢體缺血再灌注后肺損傷的保護作用及其機制[J];吉林大學學報(醫(yī)學版);2016年02期

2 李開濟;賀寶玲;盧秋玲;門秀麗;趙利軍;;缺血后處理減輕大鼠肢體缺血再灌注后肺損傷的實驗研究[J];天津醫(yī)藥;2016年04期

3 ;Effects of penehyclidine hydrochloride in small intestinal damage caused by limb ischemia-reperfusion[J];World Journal of Gastroenterology;2011年02期

【共引文獻】

相關期刊論文 前10條

1 趙利軍;李開濟;吳靜;門秀麗;;缺血后處理對大鼠肢體缺血再灌注后腎細胞凋亡的抑制作用[J];吉林大學學報(醫(yī)學版);2017年04期

2 劉瑤;趙雅寧;李建民;陳長香;劉宇;;P38MAPK通路對腦缺血后處理大鼠海馬自噬的影響[J];西安交通大學學報(醫(yī)學版);2017年04期

3 趙利軍;李開濟;吳靜;門秀麗;;缺血后處理對止血帶休克致腎損傷的作用[J];山西醫(yī)科大學學報;2017年04期

4 李培磊;劉芳;傅志仁;;NLRP3炎性小體在器官缺血再灌注損傷中的作用[J];解放軍醫(yī)學雜志;2017年02期

5 熊響清;王良榮;陳祥建;楊建平;;抑制鞘氨醇激酶-1/1-磷酸鞘氨醇信號通路減少大鼠肢體缺血再灌注肺損傷[J];中華實驗外科雜志;2016年11期

6 康于慶;黃再青;劉慧;何志強;朱俊峰;古妙寧;;PI3K/Akt信號通路在鹽酸戊乙奎醚抑制肢體缺血再灌注大鼠腸組織自噬中的作用[J];中華麻醉學雜志;2016年08期

7 趙利軍;李開濟;盧秋玲;門秀麗;;缺血后處理對大鼠肢體缺血再灌注后肺損傷的保護作用及其機制[J];吉林大學學報(醫(yī)學版);2016年02期

8 梁鵬沖;鄧芳;王瑜清;張瓊;石亞軍;樊冬梅;王大軍;王厚清;趙寧軍;燕憲亮;許鐵;;鹽酸戊乙奎醚對創(chuàng)傷性休克兔腸組織誘導型一氧化氮合酶活性和一氧化氮含量的影響[J];國際麻醉學與復蘇雜志;2016年01期

9 李開濟;賀寶玲;盧秋玲;門秀麗;趙利軍;;缺血后處理減輕大鼠肢體缺血再灌注后肺損傷的實驗研究[J];天津醫(yī)藥;2016年04期

10 康于慶;李躍兵;黃再青;古妙寧;林春水;;鹽酸戊乙奎醚對大鼠小腸組織自噬的影響[J];中國現(xiàn)代醫(yī)學雜志;2015年09期

【二級參考文獻】

相關期刊論文 前9條

1 蘇建華;陳玉芳;丁新生;唐金榮;肖杭;林偉;;血必凈對大鼠腦缺血再灌注損傷的保護作用及其機制[J];吉林大學學報(醫(yī)學版);2015年05期

2 于健;李睿;王琦;;右美托咪啶預處理對下肢缺血再灌注性肺損傷及一氧化氮/內(nèi)皮素-1失衡的影響[J];天津醫(yī)藥;2015年05期

3 趙利軍;門秀麗;李宏杰;孔小燕;吳靜;劉麗華;;缺血后適應減輕大鼠肢體缺血再灌注后的心肌損傷[J];中國病理生理雜志;2012年10期

4 趙利軍;孔小燕;門秀麗;李宏杰;吳靜;劉麗華;張連元;;丹參對大鼠肢體缺血再灌注后多器官水腫的預防作用[J];天津醫(yī)藥;2012年08期

5 王煜霞;宋曉榮;姬明麗;王建剛;郭勇;劉自國;;左心缺血再灌注大鼠急性肺損傷組織中ICAM-1與TNF-α的表達[J];鄭州大學學報(醫(yī)學版);2012年04期

6 ;Effects of penehyclidine hydrochloride in small intestinal damage caused by limb ischemia-reperfusion[J];World Journal of Gastroenterology;2011年02期

7 薛榮亮;何家璇;王寧;姚鳳珍;呂建瑞;吳剛;;細胞信號通路與DNA損傷修復的關系及在大鼠全腦缺血再灌注中的作用(英文)[J];Neuroscience Bulletin;2009年03期

8 王曉剛;闞世廉;張寶貴;王學謙;于順祿;田亞力;王世忠;孫銳;;運動終板在骨骼肌缺血再灌注損傷中的病理變化[J];中國修復重建外科雜志;2006年11期

9 陳群,曾因明;Anisodamine protects against neuronal death following cerebral ischemia in gerbils[J];Chinese Medical Journal;2000年07期

【相似文獻】

相關期刊論文 前10條

1 王洪震,沈施仁,董啟榕;肢體缺血再灌注時炎性介質(zhì)的表達和臨床意義[J];中國血液流變學雜志;2005年01期

2 朱娜;張連元;董淑云;李穎;鄂麗;孫穎;;肢體缺血再灌注大鼠血漿腎上腺髓質(zhì)素變化的實驗研究[J];華北煤炭醫(yī)學院學報;2009年02期

3 張江鋒;覃曉;李U，

本文編號：2196614