本文選題：高遷移率族蛋白 + 膀胱癌細(xì)胞��； 參考：《中國(guó)腫瘤生物治療雜志》2017年03期

【摘要】：目的:探討高遷移率族蛋白1(high-mobility group box 1,HMGB1)對(duì)膀胱癌T24株細(xì)胞的增殖、凋亡及惡性生物學(xué)行為的影響及其潛在作用機(jī)制。方法:收集2014年12月至2016年1月期間重慶醫(yī)科大學(xué)附屬第一醫(yī)院泌尿外科病區(qū)手術(shù)切除的20例膀胱癌以及相應(yīng)癌旁組織,免疫組化方法檢測(cè)膀胱癌和癌旁組織HMGB1表達(dá)差異;應(yīng)用RNAi處理T24細(xì)胞并分為空白對(duì)照組(Blank)、陰性對(duì)照組(NC)和干擾組(si HMGB1);CCK-8、流式細(xì)胞術(shù)、劃痕實(shí)驗(yàn)和Transwell侵襲實(shí)驗(yàn)分別檢測(cè)HMGB1敲低后對(duì)T24細(xì)胞增殖、凋亡、周期、遷移以及侵襲能力的影響;Western blotting檢測(cè)不同膀胱癌細(xì)胞株BIU-87和T24細(xì)胞的HMGB1表達(dá)水平以及敲低HMGB1對(duì)T24細(xì)胞惡性生物學(xué)行為相關(guān)蛋白的影響。結(jié)果:與癌旁組織相比,HMGB1在膀胱癌組織處于高表達(dá)狀態(tài)[(67.33±4.91)vs(12.00±3.79),P0.05]。與NC組和Blank組相比,si HMGB1組細(xì)胞增殖受抑制(P0.05);流式細(xì)胞術(shù)提示敲低HMGB1后細(xì)胞凋亡率增加,細(xì)胞周期阻滯在G0/G1期;劃痕實(shí)驗(yàn)及Transwell侵襲實(shí)驗(yàn)顯示,敲低HMGB1后細(xì)胞遷移(P0.05)以及侵襲[穿膜細(xì)胞數(shù):(16.33±1.45)vs(35.00±1.53)、(34.00±2.08)個(gè),均P0.05]能力減弱;Western blotting結(jié)果顯示敲低后E-鈣黏著蛋白表達(dá)上調(diào)(P0.05),N-鈣黏著蛋白、波形蛋白、基質(zhì)金屬蛋白酶(metrix metalloproteinase,MMP)-2、MMP-9、細(xì)胞周期蛋白D1、c-Myc、β-聯(lián)蛋白表達(dá)下調(diào)(均P0.05)。結(jié)論:HMGB1可通過(guò)促進(jìn)膀胱癌細(xì)胞EMT進(jìn)而增強(qiáng)其惡性生物學(xué)行為,其機(jī)制可能是通過(guò)β-聯(lián)蛋白信號(hào)通路介導(dǎo)。
[Abstract]:Objective: to investigate the effects of high mobility group protein 1(high-mobility group box 1 HMGB1 on the proliferation, apoptosis and malignant biological behavior of bladder cancer cell line T24. Methods: from December 2014 to January 2016, 20 cases of bladder cancer and its adjacent tissues were collected from the urology ward of the first affiliated Hospital of Chongqing Medical University. The expression of HMGB1 in bladder cancer and adjacent tissues was detected by immunohistochemical method. T24 cells were treated with RNAi and divided into blank control group, negative control group and interference group. Flow cytometry, scratch test and Transwell invasion assay were used to detect the proliferation, apoptosis and cycle of T24 cells after HMGB1 knockout. The expression of HMGB1 in different bladder cancer cell lines BIU-87 and T24 cells was detected by Western blotting and the effect of knock down HMGB1 on the expression of proteins associated with malignant biological behavior of T24 cells was evaluated by Western blotting. Results: the expression of HMGB1 in bladder cancer tissues was higher than that in paracancerous tissues [67.33 鹵4.91)vs(12.00 鹵3.79 P 0.05]. Compared with NC group and Blank group, cell proliferation was inhibited in HMGB1 group (P 0.05), apoptosis rate was increased and cell cycle was blocked in G0/G1 phase after knockdown with low HMGB1 by flow cytometry. The ability of cell migration (P0.05) and invasion (16.33 鹵1.45)vs(35.00 鹵1.53 鹵34.00 鹵2.08) of cell migration after knockdown (P0.05) was decreased. The results of Western blotting showed that the expression of E-cadherin up-regulated the expression of P0.05 N-cadherin and vimentin after knockout. The expression of matrix metalloproteinase (MMP), MMP- 2, MMP-9, and cyclin D1, c-Myc, 尾-binding protein were down-regulated (all P 0.05). ConclusionHMGB1 can enhance the malignant biological behavior of bladder cancer cells by promoting EMT, and its mechanism may be mediated by 尾 -integrin signaling pathway.
【作者單位】： 重慶醫(yī)科大學(xué)附屬第一醫(yī)院泌尿外科;重慶醫(yī)科大學(xué)附屬第一醫(yī)院重慶市分子腫瘤及表觀遺傳學(xué)重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(No.81372758)~~
【分類(lèi)號(hào)】：R737.14

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 青云;英國(guó)科學(xué)家辨別出與膀胱癌有關(guān)的基因[J];中國(guó)處方藥;2004年08期

2 ;特殊激素可導(dǎo)致男性患膀胱癌幾率升高[J];中國(guó)社區(qū)醫(yī)師(綜合版);2007年11期

3 丁虹彬，，余雪軒;培養(yǎng)膀胱癌細(xì)胞的新方法[J];南京醫(yī)學(xué)院學(xué)報(bào);1994年01期

4 張曉峰;劉訓(xùn)勤;;一種新型膀胱癌快速診斷裝置[J];醫(yī)療衛(wèi)生裝備;2013年10期

5 孫宏志,凡杰,唐孝達(dá);自穩(wěn)定反義IGF1R基因片段對(duì)膀胱癌細(xì)胞的影響[J];中華泌尿外科雜志;2000年05期

6 章小平,楊紅枚,魯功成;膀胱癌穩(wěn)定表達(dá)多藥耐受相關(guān)蛋白亞克隆的生物學(xué)特征[J];中華實(shí)驗(yàn)外科雜志;2000年03期

7 漆少廷,徐錫坤,王心如;膀胱癌的分子生物學(xué)研究進(jìn)展[J];國(guó)外醫(yī)學(xué).泌尿系統(tǒng)分冊(cè);2000年04期

8 章小平,楊紅枚,魯功成;流式細(xì)胞儀在膀胱癌多藥耐受研究中的應(yīng)用[J];臨床泌尿外科雜志;2000年09期

9 楊莉,汪少娟,楊業(yè)金,陳忠,吳燕麗;抗腫瘤藥對(duì)膀胱癌細(xì)胞產(chǎn)生的免疫抑制因子作用的影響[J];藥物流行病學(xué)雜志;2000年03期

10 趙陽(yáng),陳旭峰,曹浩明;人膀胱癌體外藥物敏感性預(yù)測(cè)與臨床應(yīng)用[J];中國(guó)腫瘤臨床;2001年11期

相關(guān)會(huì)議論文 前10條

1 張永春;谷江;孫發(fā);石家齊;;傳統(tǒng)醫(yī)學(xué)在膀胱癌治療中的意義[A];2010年貴州省泌尿外科學(xué)術(shù)會(huì)議論文集[C];2010年

2 潘春武;沈周俊;唐小瑩;王e

本文編號(hào)：1860319