本文選題：去勢抵抗性前列腺癌　切入點：LNCaP-AI細胞　出處：《中國病理生理雜志》2016年07期

【摘要】：目的:探討SHARPIN對去勢抵抗性前列腺癌細胞LNCaP-AI壞死性凋亡關(guān)鍵因子Rip1的調(diào)控作用,以及對細胞壞死性凋亡的影響。方法:將LNCaP-AI細胞分為TNF-α+Z-VAD(caspase抑制劑)處理組與TNF-α+Z-VAD+Nec-1(Rip1抑制劑)處理組,應(yīng)用MTS檢測各組細胞的活力,研究壞死性凋亡機制在誘導(dǎo)LNCaP-AI細胞死亡中的作用。將LNCaP-AI細胞分為陰性對照組和SHARPIN干擾(si-SHARPIN)組,RT-qPCR驗證抑制效率,通過免疫熒光等技術(shù)進一步探討SHARPIN調(diào)控壞死性凋亡的具體分子機制。結(jié)果:與對照組相比,TNF-α+Z-VAD處理組的LNCaP-AI細胞活力下降28%(P0.05),而TNF-α+Z-VAD+Nec-1處理組的細胞在Rip1被抑制后,細胞活力無明顯改變。在LNCaP-AI細胞中,通過siRNA抑制SHARPIN表達后,Rip1表達水平上調(diào),同時,LNCaP-AI細胞壞死性凋亡比例升高。結(jié)論:LNCaP-AI細胞可通過壞死性凋亡機制誘導(dǎo)死亡,下調(diào)SHARPIN可能通過激活Rip1增強LNCaP-AI細胞壞死性凋亡。
[Abstract]:Aim: to investigate the regulatory effect of SHARPIN on necrotizing apoptosis of LNCaP-AI cells and the effect of Rip1 on necrotic apoptosis in Ovariectomized prostate cancer cells.Methods: LNCaP-AI cells were divided into two groups: TNF- 偽 Z-VAD(caspase inhibitor (TNF- 偽 Z-VAD Nec-1(Rip1 inhibitor) and TNF- 偽 Z-VAD Nec-1(Rip1 inhibitor (TNF- 偽 Z-VAD Nec-1(Rip1 inhibitor). MTS was used to detect the cell viability and to study the role of necrotizing apoptosis in inducing the death of LNCaP-AI cells.LNCaP-AI cells were divided into negative control group and SHARPIN interference si-SHARPIN group to verify the inhibition efficiency of RT-qPCR. The specific molecular mechanism of SHARPIN regulating necrotizing apoptosis was further investigated by immunofluorescence technique.In LNCaP-AI cells, siRNA inhibited the expression of SHARPIN and up-regulated the expression of rip1, while the percentage of necrotic apoptosis in LNCaP-AI cells increased.Conclusion death can be induced by necrotic apoptosis in the cell line of 1: LNCaP-AI, and down-regulation of SHARPIN may enhance necrotizing apoptosis of LNCaP-AI cells by activating Rip1.
【作者單位】： 中山大學(xué)孫逸仙紀念醫(yī)院泌尿外科;中山大學(xué)孫逸仙紀念醫(yī)院小兒外科;中山大學(xué)孫逸仙紀念醫(yī)院急診外科;南方醫(yī)科大學(xué)珠江醫(yī)院泌尿外科;中山大學(xué)附屬博濟醫(yī)院泌尿外科;
【基金】：國家自然科學(xué)基金資助項目(No.81272807) 廣東省科技計劃資助項目(No.2014A020212018) 廣東省醫(yī)學(xué)研究資助項目(No.A2015113)
【分類號】：R737.25
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本文編號：1699851