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  本文選題：膀胱移行細胞癌　切入點：熒光原位雜交　出處：《吉林大學》2014年碩士論文　論文類型：學位論文

【摘要】：目的：利用熒光原位雜交技術(shù)，測出膀胱移行細胞癌患者尿脫落細胞中3號染色體、7號染色體、17號染色體及9號染色體p16基因突變值，探討突變值與膀胱移行細胞癌病理分級之間是否存在相關(guān)性，為膀胱移行細胞癌的早期診斷及分級診斷提供一種新的方法。 方法： 1、選取實驗對象100例（2012年10月至2013年5月期間吉林大學中日聯(lián)誼醫(yī)院確診膀胱占位性病變，且脫落細胞檢測發(fā)現(xiàn)尿中存在脫落細胞）。2、進行熒光原位雜交檢查，并記錄尿脫落細胞中3號染色體、7號染色體、17號染色體及9號染色體p16基因突變值。3、術(shù)中留取組織標本（已獲得醫(yī)學倫理委員會認可），HE染色后，行組織病理學檢查，確定腫瘤分級。分級標準采用WHO2004分級法。 結(jié)果：FISH檢測所有患者尿脫落細胞均可見有熒光標記，且3號染色體、17號染色體異常細胞個數(shù)及3號染色體、7號染色體、17號染色體異常細胞內(nèi)非整倍染色體的個數(shù)（即突變值）與腫瘤病理分級呈正相關(guān)性(Sig＜0.005)； 結(jié)論：3號、17號染色體異常細胞個數(shù)及3號、7號、17號染色體異常細胞內(nèi)非整倍染色體的個數(shù)（即突變值）與膀胱移行細胞癌病理分級呈正相關(guān)性，9號染色體p16基因的丟失與病理分級之間無明顯相關(guān)性�？烧J為熒光原位雜交技術(shù)檢測能夠區(qū)分不同病理級別的膀胱移行細胞癌，，可用于臨床早期病理分級診斷。
[Abstract]:Objective: to detect the mutation value of p16 gene on chromosome 3, chromosome 7, chromosome 17 and chromosome 9 in urine exfoliated cells of patients with transitional cell carcinoma of bladder by fluorescence in situ hybridization. To explore the correlation between mutation value and pathological grade of bladder transitional cell carcinoma (TCC), and to provide a new method for early diagnosis and grading diagnosis of TCC. Methods:. 1. From October 2012 to May 2013, 100 patients (from October 2012 to May 2013) were selected to confirm the bladder occupying lesions, and the exfoliated cells were detected by fluorescence in situ hybridization, and the exfoliated cells were found to exist in the urine. The mutation value of p16 gene on chromosome 3, chromosome 7, chromosome 17 and chromosome 9 in urine exfoliated cells was recorded. The grade of tumor was determined. The standard of classification was WHO2004. Results the urine exfoliated cells of all the patients were detected with fluorescent markers by the urine sample. The number of abnormal cells on chromosome 3 and 17 and the number of aneuploidy chromosomes in abnormal cells of chromosome 3, 7 and 17 were positively correlated with pathological grade of tumor. Conclusion: the number of abnormal cells on chromosome 3, 17 and the number of aneuploidy chromosomes in abnormal cells of chromosome 3, 7 and 17 are positively correlated with the pathological grade of bladder transitional cell carcinoma. There is no significant correlation between loss and pathological grade. Fluorescence in situ hybridization can be used to distinguish transitional cell carcinoma of bladder with different pathological grades. It can be used for clinical early pathological grading diagnosis.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R737.14

【參考文獻】

相關(guān)期刊論文 前4條

1 趙揚;楊曉峰;郝小強;;多肽CSNRDARRC與膀胱癌細胞結(jié)合親和性及其位點的研究[J];現(xiàn)代泌尿生殖腫瘤雜志;2011年02期

2 薛軍,林茂芳;Survivin基因在血液腫瘤細胞株細胞的表達[J];實用腫瘤雜志;2003年06期

3 李莉;丁奕星;徐苓;馬妍慧;張良;;染色體3、7、17號和9p21位點在膀胱尿路上皮癌中的畸變情況及其臨床意義[J];診斷學理論與實踐;2012年01期

4 肖澤均,張建軍,鄭閃,李長嶺,何祖根,程書鈞,高燕寧;膀胱移行細胞癌3號染色體短臂上抑癌基因雜合性缺失的研究[J];中華醫(yī)學雜志;2002年20期

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