本文選題：前列腺癌　切入點：微小RNA　出處：《中華男科學雜志》2017年07期 　論文類型：期刊論文

【摘要】：目的:探討循環(huán)微小RNA 152(miR-152)對前列腺癌生化復發(fā)早期預測的價值。方法:收集將要進行前列腺癌手術的患者66例(生化復發(fā)組:35例,術后2年內(nèi)生化復發(fā);未生化復發(fā)組:31例,術后2年內(nèi)未生化復發(fā)),另擇年齡相匹配的31例健康男性作為健康對照組。用qRT-PCR法檢測前列腺癌患者和健康對照組的循環(huán)miR-152表達水平。應用ROC曲線分析循環(huán)miR-152對前列腺癌早期生化復發(fā)的診斷價值。分析復發(fā)組循環(huán)miR-152表達水平與臨床病理參數(shù)(年齡、術前PSA水平、Gleason積分、臨床分期、骨轉移)之間的相關性。結果:循環(huán)miR-152在前列腺癌術后患者(未生化復發(fā)及生化復發(fā))與健康對照組的表達差異有統(tǒng)計學意義(t=-5.212,P=0.001)。前列腺癌術后生化復發(fā)組患者循環(huán)miR-152的表達量較未生化復發(fā)組患者低,差異有統(tǒng)計學意義(t=-5.727,P=0.001)。循環(huán)miR-152對前列腺癌術后生化復發(fā)早期預測的ROC曲線下面積(AUC)為0.906(95%CI,0.809~0.964),靈敏度和特異度分別為91.4%、80.6%。循環(huán)miR-152表達水平與Gleason積分、臨床分期、有無生化復發(fā)和骨轉移相關(P均0.05);隨著Gleason積分、臨床分期的增加,循環(huán)miR-152的表達水平下降。但miR-152表達量與患者年齡、術前PSA無關(P均0.05)。結論:循環(huán)miR-152表達水平在前列腺癌術后生化復發(fā)患者中顯著降低,可能成為前列腺癌生化復發(fā)早期預測的分子標志物。
[Abstract]:Objective: to investigate the value of circulating minimal RNA 152miR-152 in early prediction of biochemical recurrence of prostate cancer. Methods: 66 patients (35 cases of biochemical recurrence group, 35 cases of biochemical recurrence group and 31 cases of non-biochemical recurrence group) were collected. The circulating miR-152 expression in prostate cancer patients and healthy control group was detected by qRT-PCR method. The circulating miR-152 expression was analyzed by ROC curve. Diagnostic value of early biochemical recurrence of prostate cancer: analysis of circulating miR-152 expression and clinicopathologic parameters (age, age). Preoperative PSA level, Gleason score, clinical stage, Results: the expression of circulating miR-152 in postoperative patients with prostate cancer was significantly different from that in healthy control group. The expression of cyclic miR-152 was lower than that of non-biochemically relapsed patients. The area under the ROC curve of circulating miR-152 for the early prediction of postoperative biochemical recurrence of prostate cancer was 0.906 ~ 95CII 0.809 ~ 0.964U, the sensitivity and specificity were 91.4 ~ 80.6.The circulating miR-152 expression level, Gleason integral, clinical stage, and so on. The expression of circulating miR-152 decreased with the increase of Gleason score and clinical stage, but the expression of miR-152 was correlated with the age of the patients. Conclusion: the expression of circulating miR-152 was significantly decreased in patients with postoperative recurrence of prostate cancer and might be a molecular marker for early prediction of biochemical recurrence of prostate cancer.
【作者單位】： 溫州醫(yī)科大學檢驗醫(yī)學院;溫州醫(yī)科大學生命科學院;寧波市第二醫(yī)院檢驗科;寧波市第二醫(yī)院泌尿外科;
【基金】：浙江省醫(yī)藥衛(wèi)生科技計劃(2014KYB233) 寧波市自然科學基金(2012A610194) 寧波市第二醫(yī)院華美研究基金(2016HMKY08)~~
【分類號】：R737.25

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